帕金森病大鼠STN和M1放电变化及相关性研究
发布时间：2018-09-08 11:32
【摘要】：本研究采用6-羟基多巴胺(6-hydroxydopamine,6-OHDA)建立帕金森病(Parkinson’s disease,PD)大鼠模型。通过多通道在体记录技术,记录在清醒静止和特定抓食运动状态下大鼠底丘脑核(subthalamic nucleus,STN)和初级运动皮质(primary motor cortex,M1)神经元的放电变化,包括锋电位(spikes)和局部场电位(local field potential,LFPs)两部分。用Offline Sorter和Neuro Explorer软件还有Matlab软件分析实验组大鼠和对照组大鼠STN和M1脑区内神经元的电生理变化以及二者的相互关系。实验目的:为深入探索PD的发病机理并找到更快更有效的治疗方法提供理论基础。实验结果:根据神经元放电模式、放电频率等指标,将采集到的STN核团的电信号分为两种类型。在清醒静止状态下,实验组大鼠的第II类神经元的放电频率(Hz)(4.29±0.87)比对照组大鼠(2.97±0.20)显著升高,实验组大鼠的第II类神经元CV(coefficient of variation)值(1.06±0.04)比对照组大鼠(0.95±0.01)显著增加;在特定抓食运动状态下,实验组大鼠的第II类神经元放电频率(Hz)(18.64±0.35)比对照组大鼠的第II类神经元放电频率(14.07±1.16)显著升高,而第I类神经元无显著变化。在清醒静止状态下,局部场电位信号各频段能量所占百分比发生显著变化,其中实验组大鼠在0.7~12Hz频段的能量占0.7~200Hz频段的百分比(70.66±0.73)较对照组大鼠(80.75±2.35)显著降低,其他频段所占百分比显著升高。在特定抓食运动状态下,实验组大鼠局部场电位在0.7~12Hz频段的能量占0.7~200Hz频段的百分比(65.27±1.48)较对照组大鼠(73.85±1.73)显著降低,实验组大鼠在12~35Hz频段能量所占百分比(21.92±0.80)较对照组大鼠(13.95±1.56)显著升高,其他频段的能量所占的百分比无明显变化。对记录到的M1核团进行神经元分类,得到两类神经元,分别为中间神经元和锥体神经元。在清醒静止状态下,实验组大鼠锥体神经元的放电频率(Hz)(3.11±0.47)比对照组大鼠(4.23±0.70)显著降低,实验组大鼠锥体神经元的CV值(0.92±0.03)比对照组大鼠(0.97±0.03)显著减小,而中间神经元电生理活动无显著变化;在特定抓食运动状态下,实验组大鼠锥体神经元的放电频率(Hz)(11.55±0.39)比对照组大鼠(16.70±1.00)显著降低,实验组大鼠中间神经元的放电频率(Hz)(17.36±1.59)较对照组大鼠(28.49±1.18)显著降低。在清醒静止状态下局部场电位信号,实验组大鼠在0.7~12Hz频段能量占0.7~200Hz频段总能量的百分比(54.65±3.17)较对照组大鼠(73.29±1.32)显著降低,实验组大鼠在12~35Hz频段能量所占总能量的百分比(28.85±2.23)较对照组大鼠(17.66±0.51)显著升高,其他频段所占的百分比无明显变化;在特定抓食运动状态下,局部场电位信号实验组大鼠在0.7~12Hz频段能量占0.7~200Hz频段总能量的百分比(41.14±1.30)较对照组大鼠(49.15±2.40)显著降低,12~35Hz频段能量占总能量的百分比(24.44±1.63)较对照组大鼠(19.47±0.82)显著升高,其他频段能量所占的百分比无明显变化。对相同时间段内记录的STN和M1的局部场电位信号进行相关性分析,得出实验组大鼠的相关系数在0.7~12 Hz频段(0.52±0.02)比对照组大鼠(0.44±0.02)显著升高,在12~35 Hz频段实验组大鼠的相关系数(0.49±0.02)比对照组大鼠(0.40±0.01)显著升高。平均相位一致性与相关系数有相似的结果:0.7~12 Hz频段实验组大鼠的平均相位一致性(0.59±0.02)比对照组大鼠(0.52±0.02)显著升高,在12~35 Hz频段实验组大鼠的平均相位一致性(0.51±0.03)比对照组大鼠(0.41±0.02)显著升高。实验结论:PD大鼠STN和M1核团的神经元单细胞放电和LFPs都发生显著变化,而且两个核团的相关性也增加,推测这两个脑区之间存在重要的纤维投射,为治疗帕金森病提供理论依据。
[Abstract]:In this study, 6-hydroxydopamine (6-OHDA) was used to establish a rat model of Parkinson's disease (PD). Neurons in subthalamic nucleus (STN) and primary motor cortex (M1) were recorded by multi-channel in vivo recording technique during waking, resting and specific grazing exercise in rats. The electrophysiological changes of neurons in STN and M1 brain regions of experimental and control rats were analyzed by Offline Sorter, Neuro Explorer and MATLAB software. The results showed that the electrical signals of STN nuclei were divided into two types according to neuronal discharge patterns and discharge frequencies. The CV (coefficient of variation) of type II neurons in the experimental group was significantly higher than that in the control group (0.95+0.01), and the firing frequency (Hz) (18.64+0.35) of type II neurons in the experimental group was significantly higher than that in the control group (14.07+1.05). The percentage of energy in each frequency band of the local field potential signal changed significantly in the waking and resting state. The percentage of energy in the 0.7-12 Hz band of the experimental group in the 0.7-200 Hz band (70.66.73) was significantly lower than that in the control group (80.75 The percentage of local field potential energy in 0.7-12 Hz band (65.27+1.48) in the experimental group was significantly lower than that in the control group (73.85+1.73), and the percentage of energy in 12-35 Hz band (21.92+0.80) in the experimental group was significantly lower than that in the control group (13.95+1.56). Two types of neurons, intermediate neurons and pyramidal neurons, were obtained by classifying the recorded M1 nuclei. The firing frequency of pyramidal neurons in the experimental group (Hz) (3.11 + 0.47) was significantly lower than that in the control group (4.23 + 0.70). The CV value of pyramidal neurons in the experimental group (0.92.03) was significantly lower than that in the control group (0.97.03), but the electrophysiological activity of the intermediate neurons was not significantly changed. The firing frequency (Hz) (11.55.39) of pyramidal neurons in the experimental group was significantly lower than that in the control group (16.70.00), and the firing frequency (Hz) (11.55.39) of pyramidal neurons in the The firing frequency of the intermediate neurons (17.36 1.59) was significantly lower than that of the control group (28.49 1.18). The percentage of the energy in the 0.7 12 Hz frequency band (54.65 3.17) of the experimental group was significantly lower than that of the control group (73.29 1.32). The frequency of the experimental group was 12 35 Hz. The percentage of segment energy in total energy (28.85 65 (49.15 + 2.40) decreased significantly, and the percentage of energy to total energy in 12-35 Hz band (24.44 + 1.63) was significantly higher than that in control group (19.47 + 0.82). There was no significant change in the percentage of energy in other bands. The correlation coefficient of the experimental group was significantly higher than that of the control group in the frequency band of 0.7-12 Hz (0.52+0.02) and in the frequency band of 12-35 Hz (0.49+0.02) than that of the control group (0.40+0.01). The mean phase congruence (0.51 65 There is an important fiber projection between the brain regions, which provides a theoretical basis for the treatment of Parkinson's disease.
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R742.5
，
本文编号：2230391
[Abstract]:In this study, 6-hydroxydopamine (6-OHDA) was used to establish a rat model of Parkinson's disease (PD). Neurons in subthalamic nucleus (STN) and primary motor cortex (M1) were recorded by multi-channel in vivo recording technique during waking, resting and specific grazing exercise in rats. The electrophysiological changes of neurons in STN and M1 brain regions of experimental and control rats were analyzed by Offline Sorter, Neuro Explorer and MATLAB software. The results showed that the electrical signals of STN nuclei were divided into two types according to neuronal discharge patterns and discharge frequencies. The CV (coefficient of variation) of type II neurons in the experimental group was significantly higher than that in the control group (0.95+0.01), and the firing frequency (Hz) (18.64+0.35) of type II neurons in the experimental group was significantly higher than that in the control group (14.07+1.05). The percentage of energy in each frequency band of the local field potential signal changed significantly in the waking and resting state. The percentage of energy in the 0.7-12 Hz band of the experimental group in the 0.7-200 Hz band (70.66.73) was significantly lower than that in the control group (80.75 The percentage of local field potential energy in 0.7-12 Hz band (65.27+1.48) in the experimental group was significantly lower than that in the control group (73.85+1.73), and the percentage of energy in 12-35 Hz band (21.92+0.80) in the experimental group was significantly lower than that in the control group (13.95+1.56). Two types of neurons, intermediate neurons and pyramidal neurons, were obtained by classifying the recorded M1 nuclei. The firing frequency of pyramidal neurons in the experimental group (Hz) (3.11 + 0.47) was significantly lower than that in the control group (4.23 + 0.70). The CV value of pyramidal neurons in the experimental group (0.92.03) was significantly lower than that in the control group (0.97.03), but the electrophysiological activity of the intermediate neurons was not significantly changed. The firing frequency (Hz) (11.55.39) of pyramidal neurons in the experimental group was significantly lower than that in the control group (16.70.00), and the firing frequency (Hz) (11.55.39) of pyramidal neurons in the The firing frequency of the intermediate neurons (17.36 1.59) was significantly lower than that of the control group (28.49 1.18). The percentage of the energy in the 0.7 12 Hz frequency band (54.65 3.17) of the experimental group was significantly lower than that of the control group (73.29 1.32). The frequency of the experimental group was 12 35 Hz. The percentage of segment energy in total energy (28.85 65 (49.15 + 2.40) decreased significantly, and the percentage of energy to total energy in 12-35 Hz band (24.44 + 1.63) was significantly higher than that in control group (19.47 + 0.82). There was no significant change in the percentage of energy in other bands. The correlation coefficient of the experimental group was significantly higher than that of the control group in the frequency band of 0.7-12 Hz (0.52+0.02) and in the frequency band of 12-35 Hz (0.49+0.02) than that of the control group (0.40+0.01). The mean phase congruence (0.51 65 There is an important fiber projection between the brain regions, which provides a theoretical basis for the treatment of Parkinson's disease.
【学位授予单位】：山东师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R742.5
，
本文编号：2230391
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