黄芪总苷和人参皂苷降低缺血性脑卒中患者脑脊液中谷氨酸、磷酸化Tau

发布时间：2018-10-14 16:49

【摘要】：目的:通过观察黄芪总苷和人参皂苷对缺血性脑卒中诱发的缺血缺氧强应激后老年受试者谷氨酸浓度和Tau超磷酸化的影响,探讨两者对Tau超磷酸化的调节,并比较其作用途径,以期为改善缺血性脑卒中诱发的认知障碍的机制提供研究思路。方法:采用2×3析因设计:即缺血性脑卒中(无卒中、缺血性脑卒中)和药物(静脉注射生理盐水、人参皂苷、黄芪总苷)的所有组合。干预后取脑脊液,HPLC测Glu含量,Western-blotting测p-AT8Ser202和GSK-3β1H8含量。结果:缺血性脑卒中可增加脑脊液内谷氨酸的浓度(P0.05);黄芪总苷可降低缺血性脑卒中诱发的过度升高的谷氨酸浓度,且两者有相减效果(P0.05);而人参皂苷对脑脊液中谷氨酸的浓度无明显影响(P0.05)。缺血性脑卒中可增加脑脊液内p-AT8Ser202以及促进Tau超磷酸化的调控蛋白GSK-3β1H8的表达(P0.05);黄芪总苷和人参皂苷可减缓脑脊液内p-AT8Ser202及促进其磷酸化的GSK-3β1H8表达上调(P0.05);两者效果相似且和缺血性脑卒中诱发的缺血缺氧强应激的影响呈相减效果(P0.05)。结论:黄芪总苷和人参皂苷可降低缺血性脑卒中患者脑脊液中谷氨酸及磷酸化Tau浓度,缓解缺血性脑卒中诱发的缺血缺氧强应激。
[Abstract]:Aim: to observe the effects of total astragalus glycosides and ginsenosides on the concentration of glutamate and Tau hyperphosphorylation in elderly subjects after ischemic and hypoxic stress induced by ischemic stroke, and to explore the regulation of Tau hyperphosphorylation by both of them and to compare their mechanisms. In order to improve the mechanism of cognitive impairment induced by ischemic stroke. Methods: 2 脳 3 factorial design was used: all combinations of ischemic stroke (no stroke, ischemic stroke) and drugs (intravenous injection of normal saline, ginsenoside, total astragaloside). After intervention, cerebrospinal fluid (CSF) was collected, Glu content was measured by HPLC, p-AT8Ser202 and GSK-3 尾 1H8 were measured by Western-blotting. Results: ischemic stroke increased the concentration of glutamate in cerebrospinal fluid (P0.05), total astragalus glycosides decreased the excessive increase of glutamate concentration induced by ischemic stroke. There was no significant effect of ginsenoside on the concentration of glutamate in cerebrospinal fluid (P0.05). Ischemic stroke increased the expression of p-AT8Ser202 and GSK-3 尾 1H8, a regulatory protein that promotes Tau hyperphosphorylation in cerebrospinal fluid (P0.05), and increased the expression of p-AT8Ser202 and phosphorylated GSK-3 尾 1H8 in cerebrospinal fluid by total astragaloside and ginsenoside (P0.05). And the effects of ischemic and hypoxic stress induced by ischemic stroke were subtracted (P0.05). Conclusion: total astragalus glycosides and ginsenosides can reduce the concentration of glutamate and phosphorylated Tau in cerebrospinal fluid of patients with ischemic stroke and relieve the ischemic and hypoxic stress induced by ischemic stroke.
【作者单位】：咸宁市中心医院湖北科技学院附属第一医院神经内科;
【分类号】：R743.3

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