神经胶质瘤与博尔纳病病毒感染相关性研究

发布时间：2018-11-02 18:59

【摘要】：目的:研究贵州省遵义地区神经胶质瘤患者中是否存在博尔纳病病毒(BDV)感染;探讨BDV P40基因片段的特点,以及BDV阳性神经胶质瘤患者的临床表现特点。方法:收集遵义医学院附属医院神经外科2015年10月至2016年09月行神经胶质瘤切除术患者肿瘤组织、外周血样本各22例,同期重度颅脑外伤行颅内减压术患者脑组织级体检中心健康体检者外周血样本24例;采用实时荧光定量反转录聚合酶链式反应(RT-PCR)法分别检测22例神经胶质瘤患者术后肿瘤组织及其外周血淋巴细胞(PBL)、24例脑外伤颅内减压术后脑组织、24例健康体检者PBL中的BDV P40基因片段,阳性标本进行基因测序及分析,总结BDV P40阳性者临床表现特点。结果:神经胶质瘤组肿瘤组织中BDV P40的阳性率为18.2%,颅内减压术组脑组织中BDV P40的阳性率为0%,两组相比差异具有统计学意义(P0.05);神经胶质瘤组PBL中BDV P40的阳性率为9.1%,健康体检者组PBL中BDV P40的阳性率为0%,两组相比差异不具有统计学意义(P0.05);其中,有2例胶质瘤患者肿瘤组织及PBL均检测出BDV P40基因片段,提示脑组织及PBL中的BDV P40检测结果不完全对等,且肿瘤组织中该病毒基因片段检出率更高。本实验扩增的目的基因片段为BDV P40(nt242-320),与标准株Strain V和流行株He/80相比同源性分别为为97.2%、100%;较Strain V对比,3个碱基发生了点突变(nt262GC、nt303 CT及nt313 AG),突变率为3.8%;与He/80对比,2个碱基发生了点突变(262 nt262GC、nt313AG),突变率为2.5%。BDV P40阳性患者临床表现以头痛、头昏为主,符合颅内肿瘤患者临床表现,不具有差异性(P0.05)。结论:贵州遵义地区神经胶质瘤患者中存在BDV感染,BDV感染可能与神经胶质瘤的发生相关;该地区BDV P40基因片段保守、稳定,可作为检测BDV感染的指标之一;BDV P40阳性的神经胶质瘤患者临床表现与BDV P40阴性的神经胶质瘤患者临床表现无差异性。
[Abstract]:Objective: to study the presence of Borna disease virus (BDV) infection in glioma patients in Zunyi, Guizhou province, and to investigate the characteristics of BDV P40 gene fragment and the clinical manifestations of BDV positive glioma patients. Methods: tumor tissues were collected from neurosurgery department of affiliated hospital of Zunyi Medical College from October 2015 to September 2016. The peripheral blood samples of 24 patients with severe craniocerebral trauma undergoing craniocerebral decompression were studied. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect tumor tissues and peripheral blood lymphocytes (PBL),) in 22 patients with glioma after intracranial decompression. The BDV P40 gene fragment in PBL of 24 healthy persons was sequenced and analyzed, and the clinical features of BDV P40 positive patients were summarized. Results: the positive rate of BDV P40 was 18.2g in glioma group and 0 in intracranial decompression group (P0.05). The positive rate of BDV P40 in PBL was 9.1 in glioma group and 0 in PBL in healthy control group. There was no significant difference between the two groups (P0.05). BDV P40 gene fragments were detected in tumor tissues and PBL of two patients with glioma, indicating that the detection results of BDV P40 in brain tissues and PBL were not identical, and the detection rate of P40 gene fragments in tumor tissues was higher. The target gene fragment was BDV P40 (nt242-320). Compared with the standard strain Strain V and the epidemic strain He/80, the homology of the target gene fragment was 97.2and 100, respectively. Compared with Strain V, point mutation occurred in three bases (nt262GC,nt303 CT and nt313 AG), mutation rate was 3.8%). Compared with He/80, two bases had point mutation (262 nt262GC,nt313AG), and the mutation rate was headache and dizziness in patients with positive 2.5%.BDV P40, which was consistent with the clinical manifestations of intracranial tumor patients, but had no difference (P0.05). Conclusion: there is BDV infection in patients with glioma in Zunyi, Guizhou province, and BDV infection may be related to the occurrence of glioma, the BDV P40 gene fragment in this area is conservative and stable, and can be used as an index for detecting BDV infection. There was no difference between BDV P40 positive gliomas and BDV P40 negative gliomas.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.41

【相似文献】