人脐带间充质干细胞联合微创血肿抽吸术治疗大鼠脑出血的实验研究
发布时间：2018-11-03 15:42
【摘要】：研究背景 脑出血是指原发性非外伤性脑实质出血,也称自发性脑出血,是临床上常见的血管性疾病,发病率、死亡率和致残率均较高。除了血肿的占位效应及对周围组织的直接破坏之外,继发性脑损伤也是出血后脑损伤的主要原因,包括出血后血肿扩大压迫脑组织,引起脑组织移位和损伤,继发性炎性反应,产生的凝血素引起的凝血连锁反应,红细胞溶解产生的血红蛋白所诱导的毒性反应,补体级联反应以及神经元凋亡等方面。因此,脑出血的及时治疗十分关键。微创血肿抽吸术一直是脑出血治疗的研究热点,近年来有关干细胞移植治疗脑出血的实验研究表明,干细胞进入大鼠脑组织中,替代了神经元的功能,减轻脑出血后的炎性反应,并促进神经功能的恢复,此方法可能会成为临床治疗脑出血的新方法。目前,仍未见脐带间充质干细胞(HUC-MSCs)移植联合微创血肿抽吸术治疗脑出血的实验研究。本实验观察了HUC-MSCs移植联合微创血肿抽吸术对大鼠脑出血的疗效,旨在为临床进一步应用干细胞移植治疗脑出血提供临床实验基础。 研究目的 本实验在建立大鼠脑出血模型的基础上,探讨HUC-MSCs移植联合微创血肿抽吸术治疗大鼠脑出血后脑内P53的表达及神经功能的恢复。 研究方法 体外分离培养HUC-MSCs,移植前以BrdU标记,采用免疫细胞化学染色方法鉴定HUC-MSCs.将造模成功的120只SD大鼠随机分为脑出血组、血肿抽吸组、细胞移植组、细胞移植联合血肿抽吸组(联合治疗组),各组又分为1、3、7、14和30d5个时间点。向尾状核注入2μL Ⅳ型胶原酶建立脑出血模型,6h后将符合标准的模型,进行相应的处理后,各组分别于各时间点处死大鼠前,用改良的大鼠神经功能缺损评分(mNSS)评价大鼠的神经功能状况,后采用免疫组织化学方法检测大鼠脑出血周围P53基因的表达及HUC-MSCs在脑组织的分布,并进行统计学处理。 研究结果 1. HUC-MSCs细胞中CD29表达阳性,细胞浆为棕褐色；CD34表达阴性,细胞浆为蓝色；CD44表达阳性,细胞膜为棕褐色；CD90表达阳性,细胞浆为棕褐色；CD105表达阳性,细胞浆为棕褐色。脑出血组和血肿抽吸组未发现BrdU表达的阳性细胞,细胞移植组和联合治疗组则于出血脑区、海马及大脑皮质等部位发现较多的阳性细胞。P53在血肿侧脑组织的胞核内广泛表达,集中在血肿周围纹状体区、皮质、皮质下及海马区等部位。2.与脑出血组比较,血肿抽吸组、细胞移植组和联合治疗组的mNSS评分、P53表达量均下降(P＜0.05),并且7d时,联合治疗组的mNSS评分及P53表达量低于脑出血组和血肿抽吸组(P＜0.05),在14-30d时则低于其余3组(P＜0.05)。 研究结论 HUC-MSCs移植联合微创血肿抽吸术治疗可以显著减少大鼠脑出血后神经元的损伤,更利于神经功能的恢复。
[Abstract]:Background intracerebral hemorrhage refers to primary non-traumatic intracerebral hemorrhage, also known as spontaneous intracerebral hemorrhage, which is a common vascular disease in clinic. The morbidity, mortality and disability rate are high. In addition to the space-occupying effect of hematoma and the direct destruction of surrounding tissues, secondary brain injury is also the main cause of brain injury after hemorrhage, including the hematoma after hemorrhage expands and compresses brain tissue, causes brain tissue displacement and injury, and secondary inflammatory reaction. The coagulation chain reaction induced by hemagglutinin, the toxic reaction induced by hemoglobin induced by erythrocyte lysis, the complement cascade reaction and neuron apoptosis. Therefore, the timely treatment of cerebral hemorrhage is very important. Minimally invasive hematoma aspiration has been a hot topic in the treatment of intracerebral hemorrhage. In recent years, experimental studies on stem cell transplantation for intracerebral hemorrhage have shown that stem cells enter the brain tissue of rats and replace the function of neurons. It may be a new method for the treatment of intracerebral hemorrhage. At present, there is no experimental study of umbilical cord mesenchymal stem cell (HUC-MSCs) transplantation combined with minimally invasive hematoma aspiration in the treatment of intracerebral hemorrhage. The effect of HUC-MSCs transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats was observed in order to provide a clinical experimental basis for the further application of stem cell transplantation in the treatment of intracerebral hemorrhage. Objective to investigate the expression of p53 and the recovery of neural function after HUC-MSCs transplantation combined with minimally invasive hematoma aspiration in the treatment of intracerebral hemorrhage in rats. Methods HUC-MSCs, was isolated and cultured in vitro and labeled with BrdU before transplantation. HUC-MSCs. was identified by immunocytochemical staining. 120 SD rats were randomly divided into intracerebral hemorrhage group, hematoma aspiration group and cell transplantation combined with hematoma aspiration group (combined treatment group). Intracerebral hemorrhage model was established by injecting 2 渭 L type 鈪，
本文编号：2308223
[Abstract]:Background intracerebral hemorrhage refers to primary non-traumatic intracerebral hemorrhage, also known as spontaneous intracerebral hemorrhage, which is a common vascular disease in clinic. The morbidity, mortality and disability rate are high. In addition to the space-occupying effect of hematoma and the direct destruction of surrounding tissues, secondary brain injury is also the main cause of brain injury after hemorrhage, including the hematoma after hemorrhage expands and compresses brain tissue, causes brain tissue displacement and injury, and secondary inflammatory reaction. The coagulation chain reaction induced by hemagglutinin, the toxic reaction induced by hemoglobin induced by erythrocyte lysis, the complement cascade reaction and neuron apoptosis. Therefore, the timely treatment of cerebral hemorrhage is very important. Minimally invasive hematoma aspiration has been a hot topic in the treatment of intracerebral hemorrhage. In recent years, experimental studies on stem cell transplantation for intracerebral hemorrhage have shown that stem cells enter the brain tissue of rats and replace the function of neurons. It may be a new method for the treatment of intracerebral hemorrhage. At present, there is no experimental study of umbilical cord mesenchymal stem cell (HUC-MSCs) transplantation combined with minimally invasive hematoma aspiration in the treatment of intracerebral hemorrhage. The effect of HUC-MSCs transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats was observed in order to provide a clinical experimental basis for the further application of stem cell transplantation in the treatment of intracerebral hemorrhage. Objective to investigate the expression of p53 and the recovery of neural function after HUC-MSCs transplantation combined with minimally invasive hematoma aspiration in the treatment of intracerebral hemorrhage in rats. Methods HUC-MSCs, was isolated and cultured in vitro and labeled with BrdU before transplantation. HUC-MSCs. was identified by immunocytochemical staining. 120 SD rats were randomly divided into intracerebral hemorrhage group, hematoma aspiration group and cell transplantation combined with hematoma aspiration group (combined treatment group). Intracerebral hemorrhage model was established by injecting 2 渭 L type 鈪，
本文编号：2308223
本文链接：https://www.wllwen.com/yixuelunwen/shenjingyixue/2308223.html
最近更新
教材专著
