Notch1通路与胶质瘤细胞管道形成能力相关性研究

发布时间：2018-11-05 18:36

【摘要】：目的探索胶质瘤细胞来源的管道(glioma clls derived vessels,GCDV)的形成机制。方法将胶质瘤细胞株U87、U251、U373、SF295、T98G、SKMG-4和C6进行体外三维培养,观察其管道形成能力。Western blot检测各个胶质瘤细胞株Notch1、Dll4蛋白的表达情况。结果三维培养C6细胞单个视野下(100×)的平均管道数(25.2±5.0)个,U373为(36.4±3.20)个,U87为(19.0±2.2)个,T98G为(12.6±2.4)个,SF295为(4.0±2.)个,U251为(0.2±0.4)个,SKMG-4为0。Notch1在U87、U251、T98G、SF295、SKMG-4、C6、U373表达相关密度分别为0.34、0.21、0.79、0.04、0.28、1.75、1.19,与管道形成能力显著相关(r=0.778,P=0.019);Dll4表达相关密度与管道形成能力不相关(r=0.635,P=0.062)。结论 Notch1蛋白表达与细胞株管道形成能力密切相关,而Dll4蛋白的表达与细胞株管道形成能力有待进一步探索。
[Abstract]:Objective to explore the formation mechanism of (glioma clls derived vessels,GCDV in glioma cells. Methods Glioma cell line U87U251U373F295T98GNSKMG-4 and C6 were cultured in vitro, and the expression of Notch1,Dll4 protein in each glioma cell line was detected by. Western blot. Results the average number of tubes (25.2 卤5.0), U373 (36.4 卤3.20), U87 (19.0 卤2.2), T98G (12.6 卤2.4) and SF295 (4.0 卤2. 2) in a single field of view were (25.2 卤5.0), (36.4 卤3.20), (19.0 卤2.2), (12.6 卤2.4) and (4.0 卤2. 2) respectively. The correlation densities of U251, U251 and 0.Notch1 in U87U U251U T98GU SF295N SKMG-4, C6U U373 were 0.34, 0.21, 0.79, 0.04, 0.280.28 and 1.751.19, respectively, which were significantly correlated with the ability of pipeline formation (r = 0.778, P < 0.05), and the correlation densities of the expression of U251 and 0.Notch1 were 0.34, 0.21, 0.79, 0.04, 0.28 and 1.751.19, respectively. P0. 019); There was no correlation between the density of Dll4 expression and the ability of pipeline formation (r = 0.635P0. 062). Conclusion the expression of Notch1 protein is closely related to the ability of the cell line to form the conduit, while the expression of Dll4 protein and the ability of the cell line to form the conduit need to be further explored.
【作者单位】：深圳市南山区人民医院神经外科;中山大学肿瘤防治中心神经外科;中山大学附属第八医院深圳市福田区人民医院神经外科;
【基金】：国家自然科学基金(编号:81372685) 国家重点基础研究发展计划(973计划)(编号:2015CB755505) 广东省自然科学基金(编号:S2013040012894) 广东省科技计划项目(编号:2013B021800067,2016A02013004) 深圳市科技创新委员会项目(编号:JCYJ20140416094330210) 广东省医学科研基金(编号:A2014247)
【分类号】：R739.41

【相似文献】