CD90在人脑胶质瘤中的表达及临床意义

发布时间：2018-11-05 18:45

【摘要】：目的：胶质瘤是最常见的颅内肿瘤，据统计资料，国内胶质瘤占颅内肿瘤的35.26%~60.69%（平均44.69%），居第一位。胶质瘤呈浸润性生长，其恶性程度高、生长快、易复发。一旦发现，往往预后不佳，生存率低，严重威胁人类生命健康、正常生活工作。至今依然是所有恶性肿瘤中接受综合治理预后最差的一类中枢神经系统肿瘤。因此，迫切需要新的策略来治疗恶性神经胶质瘤。 CD90是细胞粘附分子免疫球蛋白超家族中最小的成员。它是一个重糖基化的糖蛋白，人类CD90的功能是目前尚未明确。目前推测其功能与细胞-细胞、细胞-细胞质间的作用有关，与细胞凋亡和转移、炎症、纤维化等也可能相关。表面蛋白CD90被认为是人类干细胞多能性的一个主要指标。它已在许多干细胞中和肿瘤干细胞中（造血干细胞，骨髓源性间充质干细胞，肝干细胞，以及肝肿瘤干细胞）表达。肿瘤干细胞是少数具有干细胞性质的细胞群体存在于肿瘤组织中，这些细胞具有自我更新能力以及多向分化潜能。肿瘤干细胞在肿瘤组织中存在极少，可促使肿瘤的发生和发展，也是肿瘤耐药性、复发及转移的根源。肿瘤干细胞相比正常干细胞有以下独有特点：无限的自我更新能力、高致瘤性、异质性、耐药性和转移性。近年来有研究表明，脑胶质瘤内存在少部分的肿瘤干细胞，，具有神经干细胞特性，有进行自我更新、多向分化及体内致瘤的潜力，是肿瘤发生、发展的根源。本实验采用免疫组织化学染色方法和实时荧光定量PCR法检测正常脑组织和不同级别的胶质瘤中CD90的表达，从而了解CD90是否能成为判断人脑胶质瘤恶性程度及预后的指标，为胶质瘤治疗提供新的思路。方法：选取2013年2月至2013年10月河北医科大学第二医院神经外科脑胶质瘤患者手术中切除的癌组织56例。10例脑组织取自于因外伤或脑出血而进行颅内减压术者做对照。应用免疫组织化学（IHCSP法）和实时荧光定量PCR法（RT-PCR）分别检测标本中CD90的表达，用统计软件SPSS13.0对实验所得数据进行统计分析。CD90在不同临床病理类型之间的比较用Kruskal-WallisH检验。检验水准α0.05。结果： 1免疫组织化学结果显示：CD90在正常脑组织、低级别胶质瘤和高级别胶质瘤中的表达情况呈现递增趋势。在所有正常脑组织中，CD90均未见表达。在低级别胶质瘤组，多数未见CD90表达，少部分可见CD90弱表达。在高级别胶质瘤中，CD90的表达量明显多于低级别胶质瘤，所有高级别胶质瘤中均有CD90的表达，并且表达水平较高。统计学分析显示CD90在正常脑组织、低级别和高级别胶质瘤中的表达差异有统计学意义。随着肿瘤的恶性度增高，CD90的表达量呈升高趋势。组间比较得出CD90在正常脑组织与低级别胶质瘤的差异性没有统计学意义。在正常脑组织与高级别胶质瘤的差异性有统计学意义。在低级别胶质瘤与高级别胶质瘤的差异性有统计学意义。 2RT-PCR检测结果显示：CD90在正常脑组织、低级别和高级别胶质瘤中的表达差异有统计学意义。随着肿瘤的恶性度增高，CD90的表达量呈升高趋势。组间比较得出CD90在正常脑组织与低级别胶质瘤的差异性有统计学意义。在正常脑组织与高级别胶质瘤的差异性有统计学意义。在低级别胶质瘤与高级别胶质瘤的差异性有统计学意义。结论： 1在高级别胶质瘤（WHOIII和IV级）中CD90的表达水平显著地高于低级别胶质瘤（WHO分级I和II）和正常脑组织。 2CD90具有很高的灵敏度和特异性的标志物，在所有被测试的高级别胶质瘤组织中高或中的表达水平，但在低级别胶质瘤和正常的脑组织几乎检测不到。 3在免疫组织化学方法中，发现CD90阳性细胞在高级别胶质瘤形成血管样结构。 4对比高度分化的低级别胶质瘤，高级别胶质瘤多为低分化的，因此更可能含有未分化的肿瘤干细胞。该实验中CD90在高级别胶质瘤中的高表达水平，而在低级别胶质瘤中的低表达，提示CD90可能为神经系统肿瘤干细胞的新标记。
[Abstract]:Objective: Glioma is the most common intracranial tumor. According to statistical data, glioma accounts for 35. 26% ~ 60. 69% (average 44. 69%) of intracranial tumors. Glioma is invasive and has a high degree of malignancy, rapid growth and easy relapse. Once found, the prognosis is not good, the survival rate is low, serious threat to human life health, normal life work. So far, it is still a kind of central nervous system tumor with the worst prognosis in all malignant tumors. Therefore, there is an urgent need for new strategies to treat malignant glioma. CD90 is the smallest in the expression of immune globulin in cell adhesion molecules Member. It is a highly glycosylated glycoprotein, and human CD90 has the function of not yet Clearly, it is estimated that its function is related to cell-cell, cell-cytoplasmic interaction, apoptosis and metastasis, inflammation, fibrosis, etc. Related. Surface protein CD90 is considered to be one of multipotent human stem cells Indicators. It has been found in many stem cells and in tumor stem cells (hematopoietic stem cells, bone marrow-derived mesenchymal stem cells, liver stem cells, and liver tumor stem cells). Tumor stem cells are a few cell populations with stem cell properties in tumor tissues, and these cells have self-renewal capabilities and multiple The differentiation potential. Tumor stem cells are rare in tumor tissue, can promote the occurrence and development of tumor, also is the tumor resistance, relapse and The source of metastasis. Tumor stem cells have the following unique characteristics compared to normal stem cells: unlimited self-renewal, high tumorigenicity, heterogeneity, drug resistance In recent years, it has been shown that there are a small number of tumor stem cells in gliomas, which have the characteristics of neural stem cells, have the potential of self-renewal, multi-directional differentiation and in vivo tumorigenesis, and are tumorigenesis. The expression of CD90 in normal brain tissues and different levels of gliomas was detected by immunohistochemical staining and real-time fluorescence quantitative PCR. Methods: 56 cases of cancer tissues were selected from February 2013 to October 2013 in the second hospital of Hebei Medical University. 10 cases of brain tissue were taken from traumatic or cerebral hemorrhage. The expression of CD90 in specimens was detected by immunohistochemistry (IHCSP) and real-time fluorescence quantitative PCR (RT-PCR). Statistical analysis of the obtained data. The comparison of CD90 between different clinical and pathological types is Kruska l-WallisH test Inspection and inspection Results: 1 Immunohistochemical results showed that CD90 was in normal brain tissues, low-grade gliomas and high-grade gliomas. There is an increasing trend in expression in gliomas. In normal brain tissues, no expression was found in CD90. In the low-grade glioma group, the majority were not seen in the CD The expression of CD90 in high-grade gliomas was significantly higher than that of low-grade gliomas, and in all high-grade gliomas The expression of CD90 was expressed and the level of expression was higher. Statistical analysis showed that CD90 was in normal brain tissue, low grade and high. There was a statistically significant difference in expression in gliomas. The expression level of CD90 increased and the expression level of CD90 increased. The results showed that CD90 was in normal brain tissue. There is no statistical significance in the difference of low-grade gliomas. There was a statistically significant difference in the difference from high-grade gliomas. In low-grade gliomas The results of RT-PCR showed that CD90 was in normal brain tissue and low level. There was a statistically significant difference in the differences in the expression of non-and high-level gliomas. The degree of malignancy of CD90 increased and the expression of CD90 increased. The difference between brain tissue and low-grade gliomas was statistically significant. The difference between brain tissue and high-grade gliomas is statistically significant. At low levels Glioma Conclusions: 1 The expression level of CD90 in high-grade gliomas (WHOIII and IV) was significantly higher than that in high-grade gliomas. higher sensitivity and specificity markers than low-grade gliomas (WHO grade I and II) and normal brain tissue, high in all high-grade glioma tissues tested or moderate level of expression, but almost undetectable in low-grade gliomas and normal brain tissues. In histochemical methods, CD90 positive cells were found to form a vascular-like structure in high-grade gliomas. Gliomas, high-grade gliomas, are low-differentiated and therefore more likely to contain undifferentiated tumor stem cells. The high level of expression of CD90 in high-grade gliomas,
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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