次乌头碱对脑梗死大鼠神经功能与血清中t-PA,PAI-I含量水平的影响

发布时间：2018-11-11 13:34

【摘要】：目的通过建立脑梗死大鼠模型,探讨次乌头碱对神经功能与脑梗死大鼠血清中t-PA,PAI-I含量水平的影响。方法大鼠随机分为假手术组(Sham),脑梗死组(CI),次乌头碱低剂量组(HA-L)、中剂量组(HA-M)和高剂量组(HA-H),采用线栓法建立大鼠脑动脉闭塞模型,建模成功后,次乌头碱低剂量组、中剂量组和高剂量组分别给予次乌头碱灌胃0.25 mg/kg,0.75 mg/kg与2.25 mg/kg,每天1次,共给药3 d。给药完成后,对各组大鼠进行神经功能缺损评分,ELISA检测血清中组织型纤溶酶原激活物(t-PA)和纤溶酶原激活抑制物(PAI-I)的含量水平,TTC染色检测脑梗死体积,免疫印迹法检测ICAM-1与VCAM-1蛋白表达水平。结果与脑梗死组相比,采用次乌头碱给药后,脑梗死大鼠的神经功能缺损评分显著降低,血清中t-PA与PAI-I的含量水平明显下降,脑梗死面积降低,脑组织中ICAM-1与VCAM-1蛋白表达明显下降。结论次乌头碱能够改善脑梗死大鼠的神经功能缺损状态,可能与其影响纤溶系统以及ICAM-1与VCAM-1因子表达有关。
[Abstract]:Objective to study the effect of subaconitine on nerve function and the level of t-PAP PAI-I in the serum of rats with cerebral infarction by establishing a rat model of cerebral infarction. Methods Rats were randomly divided into three groups: (CI), subaconitine low dose group (HA-L), middle dose group (HA-M) and high dose group (HA-H) in (Sham), cerebral infarction group. After the model was successfully established, the subaconitine low dose group, the middle dose group and the high dose group were given subaconitine orally for 0. 25 mg/kg,0.75 mg/kg and 2. 25 mg/kg, respectively, for 3 days. After administration, the neurological impairment scores, the levels of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-I) in serum were detected by ELISA, and the infarct volume was detected by TTC staining. The expression of ICAM-1 and VCAM-1 protein was detected by Western blot. Results compared with the cerebral infarction group, the neurological deficit score, the levels of t-PA and PAI-I in serum and the area of cerebral infarction were significantly decreased after subaconitine administration in rats with cerebral infarction. The expression of ICAM-1 and VCAM-1 protein decreased significantly in brain tissue. Conclusion subaconitine can improve the neurological deficit in rats with cerebral infarction, which may be related to its influence on fibrinolytic system and the expression of ICAM-1 and VCAM-1 factors.
【作者单位】：新疆石河子大学医学院第一附属医院神经内科;
【分类号】：R743.3

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