Cyclin D1在肌萎缩侧索硬化症转基因小鼠大脑皮层和海马中的表达
发布时间：2018-11-23 12:59
【摘要】：目的:通过检测细胞周期相关蛋白D1(Cyclin D1)在肌萎缩侧索硬化症(ALS)转基因小鼠大脑皮层和海马中的表达变化,探讨Cyclin D1表达改变与ALS发病的关系。方法:选取成年ALS小鼠和同窝野生型小鼠,于发病早、中、晚期(95 d,108 d,122 d)取材,应用免疫荧光技术检测Cyclin D1在大脑皮层和海马的表达规律及与神经元和星形胶质细胞的共定位关系,应用RT-PCR检测Cyclin D1 mRNA表达情况,应用免疫印迹法检测蛋白表达量的改变。结果:ALS小鼠和野生型小鼠大脑皮层和海马中均可检测到Cyclin D1阳性细胞,且与神经元共表达。在发病早、中、晚期,ALS小鼠大脑皮层中Cyclin D1 mRNA和蛋白表达较野生型鼠增多(P0.05,P0.01,P0.001);与同窝野生型小鼠相比,ALS小鼠海马中Cyclin D1 mRNA和蛋白在发病的早、中、晚期表达均降低(P0.05,P0.01,P0.001)。Cyclin D1阳性细胞主要分布在海马CA区(包括CA1、CA2和CA3),DG区仅散在表达。结论:Cyclin D1在ALS转基因小鼠大脑皮层和海马中表达异常,表明Cyclin D1调节的细胞周期改变与ALS大脑皮层和海马区病变密切相关。
[Abstract]:Aim: to investigate the expression of cell cycle associated protein D1 (Cyclin D1) in cerebral cortex and hippocampus of (ALS) transgenic mice with amyotrophic lateral sclerosis and to explore the relationship between the expression of Cyclin D1 and the pathogenesis of ALS. Methods: adult ALS mice and wild-type mice of the same litter were selected and collected at the early, middle and late stage of onset (95 d ~ 108 d ~ 122d). The expression of Cyclin D1 in cerebral cortex and hippocampus and its co-localization with neurons and astrocytes were detected by immunofluorescence technique. The expression of Cyclin D1 mRNA was detected by RT-PCR and the change of protein expression by Western blot. Results: Cyclin D1 positive cells were detected in cortex and hippocampus of ALS mice and wild-type mice. In the early, middle and late stage of onset, the expression of Cyclin D1 mRNA and protein in cerebral cortex of ALS mice was higher than that of wild-type mice (P0.05, P0.01, P0.001). Compared with wild-type mice, the expression of Cyclin D1 mRNA and protein in hippocampus of ALS mice decreased in the early, middle and late stages (P0.05P0.01P0.01P0.001). Cyclin D1 positive cells mainly distributed in the CA region of hippocampus (including CA1,CA2 and CA3). The DG region was only scattered in expression. Conclusion: the abnormal expression of Cyclin D1 in cortex and hippocampus of ALS transgenic mice suggests that the changes of cell cycle regulated by Cyclin D1 are closely related to the pathological changes of ALS cortex and hippocampus.
【作者单位】： 潍坊医学院附属医院;潍坊医学院;潍坊医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金(81401066) 山东省自然科学基金(ZR2012HQ021,ZR2016HM60) 山东省教育厅课题(J11LF16) 山东省医药卫生科技发展计划项目(2016WS0691,2016WS0666) 潍坊医学院大学生科技创新基金(KX2016009,KX2016015)
【分类号】：R744.8
本文编号：2351709
[Abstract]:Aim: to investigate the expression of cell cycle associated protein D1 (Cyclin D1) in cerebral cortex and hippocampus of (ALS) transgenic mice with amyotrophic lateral sclerosis and to explore the relationship between the expression of Cyclin D1 and the pathogenesis of ALS. Methods: adult ALS mice and wild-type mice of the same litter were selected and collected at the early, middle and late stage of onset (95 d ~ 108 d ~ 122d). The expression of Cyclin D1 in cerebral cortex and hippocampus and its co-localization with neurons and astrocytes were detected by immunofluorescence technique. The expression of Cyclin D1 mRNA was detected by RT-PCR and the change of protein expression by Western blot. Results: Cyclin D1 positive cells were detected in cortex and hippocampus of ALS mice and wild-type mice. In the early, middle and late stage of onset, the expression of Cyclin D1 mRNA and protein in cerebral cortex of ALS mice was higher than that of wild-type mice (P0.05, P0.01, P0.001). Compared with wild-type mice, the expression of Cyclin D1 mRNA and protein in hippocampus of ALS mice decreased in the early, middle and late stages (P0.05P0.01P0.01P0.001). Cyclin D1 positive cells mainly distributed in the CA region of hippocampus (including CA1,CA2 and CA3). The DG region was only scattered in expression. Conclusion: the abnormal expression of Cyclin D1 in cortex and hippocampus of ALS transgenic mice suggests that the changes of cell cycle regulated by Cyclin D1 are closely related to the pathological changes of ALS cortex and hippocampus.
【作者单位】： 潍坊医学院附属医院;潍坊医学院;潍坊医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金(81401066) 山东省自然科学基金(ZR2012HQ021,ZR2016HM60) 山东省教育厅课题(J11LF16) 山东省医药卫生科技发展计划项目(2016WS0691,2016WS0666) 潍坊医学院大学生科技创新基金(KX2016009,KX2016015)
【分类号】：R744.8
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