血清miR-103a、miR-30b、miR-29a相对表达量对帕金森病的诊断效能

发布时间：2018-12-05 21:16

【摘要】：目的评价血清miR-103a、miR-30b、miR-29a相对表达量在帕金森病中的诊断效能。方法采用RTPCR法检测90例帕金森病患者(观察组)及24例健康人(对照组)外周血清中miR-103a、miR-30b、miR-29a。采用受试者工作曲线法观察血清miR-103a、miR-30b、miR-29a用于诊断帕金森病的效能。结果观察组外周血清miR-103a、miR-30b、miR-29a相对表达量分别为1.12±0.10、1.57±0.37、1.23±0.09,对照组分别为0.43±0.03、0.97±0.07、0.51±0.04,观察组外周血清miR-103a、miR-30b、miR-29a相对表达量均高于对照组,P均0.05。外周血清miR-103a诊断帕金森病的AUC为0.816,选择0.715为诊断阈值时约登指数最大,此时外周血清miR-103a诊断帕金森病的灵敏度为71.3%,特异度为83.9%。外周血清miR-30b诊断帕金森病的AUC为0.789,选择1.120为诊断阈值时约登指数最大,此时外周血清miR-30b诊断帕金森病的灵敏度为70.5%,特异度为84.6%。外周血清miR-29a诊断帕金森病的AUC为0.756,选择1.001为诊断阈值时约登指数最大,此时外周血清miR-29a诊断帕金森病的灵敏度为72.1%,特异度为80.8%。结论帕金森病患者外周血清miR-103a、miR-30b、miR-29a相对表达量升高。外周血清miR-103a、miR-30b、miR-29a相对表达量在帕金森疾病中的诊断效能好。
[Abstract]:Objective to evaluate the diagnostic efficacy of serum relative expression of miR-103a,miR-30b,miR-29a in Parkinson's disease. Methods the peripheral serum miR-103a,miR-30b,miR-29a. in 90 patients with Parkinson's disease (observation group) and 24 healthy people (control group) was detected by RTPCR method. The efficacy of serum miR-103a,miR-30b,miR-29a in the diagnosis of Parkinson's disease was observed by the method of operating curve. Results the relative expression of miR-103a,miR-30b,miR-29a in peripheral blood of observation group and control group were 1.12 卤0.10 1.57 卤0.37 卤1.23 卤0.09 and 0.43 卤0.03 卤0.97 卤0.07 卤0.51 卤0.04, respectively. The relative expression of miR-29a was higher than that of control group (P < 0.05). The AUC of peripheral blood miR-103a in diagnosis of Parkinson's disease was 0.816. When 0.715 was selected as the diagnostic threshold, the Yorden index was the largest. The sensitivity and specificity of miR-103a in the diagnosis of Parkinson's disease were 71.3 and 83.9 respectively. The AUC of peripheral blood serum miR-30b for diagnosis of Parkinson's disease was 0.789, and when 1.120 was selected as diagnostic threshold, Yorden index was the largest. The sensitivity and specificity of miR-30b in peripheral blood serum for diagnosis of Parkinson's disease were 70.5 and 84.6 respectively. The AUC of peripheral blood miR-29a in diagnosis of Parkinson's disease was 0.756. When 1.001 was selected as the diagnostic threshold, the Yorden index was the largest. The sensitivity and specificity of miR-29a in the diagnosis of Parkinson's disease were 72.1 and 80.8 respectively. Conclusion the relative expression of miR-103a,miR-30b,miR-29a in peripheral blood of patients with Parkinson's disease is increased. The relative expression of miR-103a,miR-30b,miR-29a in peripheral blood is effective in the diagnosis of Parkinson disease.
【作者单位】：焦作市人民医院;
【分类号】：R742.5

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