结扎腰动脉建立大鼠脊髓缺血模型有效性的评估

发布时间：2018-12-09 12:43

【摘要】：目的结扎腰动脉建立大鼠脊髓缺血模型,为研究缺血性脊髓病、继发性脊髓损伤的发生机制提供良好的载体。方法将大鼠随机分为假手术组(仅分离出腰动脉)和脊髓缺血组(结扎所有腰动脉),于术后进行大鼠后肢运动功能BBB评分、脊髓苏木精-伊红(H-E)染色镜下观察及Caspase-3免疫组织化学检测。结果术后48h对缺血组大鼠后肢运动功能BBB评分为(9.22±0.97),与对照组比较,差别有统计学意义(P0.01);H-E染色镜下可见脊髓缺血组大鼠正常神经元数目明显减少;免疫组织化学显示Caspase-3阳性细胞呈棕黄色,脊髓缺血组阳性率远大于假手术组,差别有统计学意义(P0.01)。结论通过结扎大鼠腰动脉可以建立稳定的脊髓缺血模型,为脊髓缺血后损伤机制和预防措施的研究提供良好的载体。
[Abstract]:Objective to establish a spinal cord ischemia model by ligating lumbar artery in rats, and to provide a good carrier for studying the mechanism of ischemic myelopathy and secondary spinal cord injury. Methods Rats were randomly divided into two groups: sham operation group (only isolated lumbar artery) and spinal cord ischemia group (ligation of all lumbar arteries). The BBB score of motor function of hind limbs was evaluated after operation. Hematoxylin-eosin (H-E) staining of spinal cord was observed under microscope and Caspase-3 was detected by immunohistochemistry. Results the BBB score of hindlimb motor function in ischemic group was (9.22 卤0.97) at 48 hours after operation, which was significantly different from that in control group (P0.01). H-E staining showed that the number of normal neurons in spinal cord ischemia group was significantly decreased. Immunohistochemistry showed that Caspase-3 positive cells were brown and the positive rate of spinal cord ischemia group was much higher than that of sham operation group (P0.01). Conclusion A stable spinal cord ischemia model can be established by ligating the lumbar artery in rats, which provides a good carrier for the study of the mechanism and preventive measures of spinal cord ischemia injury.
【作者单位】：福建医科大学附属福州市第一医院神经外科;福建医科大学附属协和医院神经外科;
【分类号】：R744;R-332

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