LPL基因甲基化与脑梗死的关系
发布时间：2018-12-15 20:54
【摘要】：目的探讨血脂代谢相关基因甲基化程度与脑梗死及其相关危险因素的关系。 方法采用病例-对照研究方法随机抽取湖南汉族人群脑梗死患者及健康对照者各15名。应用亚硫酸氢盐测序技术检测LPL基因启动子区甲基化状态。 结果1.病例组和对照组相比,病例组舒张压、血浆LDL-C水平高于对照组,病例组血浆HDL-C水平低于对照组,差异有统计学意义(P0.05)。 2.病例组与对照组比较,病例组LPL基因启动子30个位点整体平均甲基化水平(CpGZ%)显著高于对照组,差异有统计学意义(P0.05)。两组各位点间的平均甲基化水平(CpG%)进行比较,位点CpG9～16,CpG20～21处病例组甲基化水平明显高于对照组,差异有统计学意义(P0.05)。 3.腔隙性脑梗死组与动脉粥样硬化性血栓性脑梗死组LPL基因启动子平均甲基化水平(CpGZ%)比较无明显差异(P0.05)。颈动脉粥样硬化组与无颈动脉粥样硬化组LPL基因启动子平均甲基化水平(CpGZ%)比较无明显差异(P0.05)。 4.所有研究对象血浆HDL-C水平与LPL基因启动子平均甲基化水平(CpGZ%)存在显著负的直线相关关系(P=0.001)。CpG9～16位点处直线相关性显著,差异有统计学意义(P0.05)。所有研究对象血浆LDL-C水平与LPL基因启动子平均甲基化水平(CpGZ%)存在显著正的直线相关关系(P=0.004),CpG10～12、CpG15、CpG20位点处两者直线相关性显著,差异有统计学意义(P0.05)。 5.年龄与LPL基因启动子平均甲基化水平(CpGZ%)存在显著正的直线相关关系(P=0.024),CpG9～16位点处直线相关性显著,差异有统计学意义(P0.05)。 6.病例组、对照组和合并组男性与女性LPL基因启动子平均甲基化水平(CpGZ%)比较无明显差异(P0.05)。男性病例组与对照组LPL基因启动子平均甲基化水平(CpGZ%)比较存在显著差异(P=0.001)。女性病例组与对照组LPL基因启动子平均甲基化水平(CpGZ%)比较存在显著差异(P=0.024)。 结论1.LPL基因启动子区高甲基化与脑梗死发病相关,与血浆低HDL-C水平及血浆高LDL-C水平相关。 2.LPL基因启动子区甲基化可能与梗死灶面积及颈动脉粥样硬化无关。 3.LPL基因启动子区甲基化水平无性别差异,随年龄增长有升高趋势。
[Abstract]:Objective to investigate the relationship between methylation of hyperlipidemia related genes and cerebral infarction and related risk factors. Methods A case-control study was conducted to randomly select 15 patients with cerebral infarction and 15 healthy controls in Hunan Han population. The methylation status of promoter region of LPL gene was detected by bisulfite sequencing. Result 1. Compared with the control group, the diastolic blood pressure and plasma LDL-C level in the case group were higher than those in the control group, and the plasma HDL-C level in the case group was lower than that in the control group (P0.05). 2. Compared with the control group, the global average methylation level (CpGZ%) of 30 sites of LPL gene promoter in the case group was significantly higher than that in the control group (P0.05). The average methylation level (CpG%) of the two groups was significantly higher than that of the control group (P0.05). 3. There was no significant difference in average methylation (CpGZ%) of LPL gene between lacunar infarction group and atherosclerotic thrombotic cerebral infarction group (P0.05). There was no significant difference in average methylation level (CpGZ%) of LPL gene promoter between carotid atherosclerosis group and non-carotid atherosclerosis group (P0.05). 4. There was a significantly negative linear correlation between plasma HDL-C level and the average methylation level (CpGZ%) of LPL gene promoter (P0. 001). The linear correlation at CpG9~16 locus was significant (P0.05). There was a significant positive linear correlation between plasma LDL-C level and average methylation level (CpGZ%) of LPL gene promoter (P0. 004), and a significant linear correlation between plasma LDL-C level and mean methylation level (CpGZ%) at CpG10~12,CpG15,CpG20 locus. The difference was statistically significant (P0.05). 5. There was a significant positive linear correlation between age and average methylation level (CpGZ%) of LPL gene promoter (P0. 024) and a significant linear correlation at CpG9~16 locus (P0.05). 6. There was no significant difference in mean methylation (CpGZ%) level of LPL gene promoter between male and female in case group, control group and combined group (P0.05). The mean methylation level (CpGZ%) of LPL gene promoter was significantly different between male and control group (P0. 001). The mean methylation level (CpGZ%) of LPL gene promoter was significantly different between female and control group (P0. 024). Conclusion hypermethylation in the promoter region of 1.LPL gene is associated with cerebral infarction, low HDL-C level and high LDL-C level. 2.LPL promoter methylation may not be associated with infarct size and carotid atherosclerosis. There was no gender difference in methylation level in promoter region of 3.LPL gene, but it tended to increase with age.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3
[Abstract]:Objective to investigate the relationship between methylation of hyperlipidemia related genes and cerebral infarction and related risk factors. Methods A case-control study was conducted to randomly select 15 patients with cerebral infarction and 15 healthy controls in Hunan Han population. The methylation status of promoter region of LPL gene was detected by bisulfite sequencing. Result 1. Compared with the control group, the diastolic blood pressure and plasma LDL-C level in the case group were higher than those in the control group, and the plasma HDL-C level in the case group was lower than that in the control group (P0.05). 2. Compared with the control group, the global average methylation level (CpGZ%) of 30 sites of LPL gene promoter in the case group was significantly higher than that in the control group (P0.05). The average methylation level (CpG%) of the two groups was significantly higher than that of the control group (P0.05). 3. There was no significant difference in average methylation (CpGZ%) of LPL gene between lacunar infarction group and atherosclerotic thrombotic cerebral infarction group (P0.05). There was no significant difference in average methylation level (CpGZ%) of LPL gene promoter between carotid atherosclerosis group and non-carotid atherosclerosis group (P0.05). 4. There was a significantly negative linear correlation between plasma HDL-C level and the average methylation level (CpGZ%) of LPL gene promoter (P0. 001). The linear correlation at CpG9~16 locus was significant (P0.05). There was a significant positive linear correlation between plasma LDL-C level and average methylation level (CpGZ%) of LPL gene promoter (P0. 004), and a significant linear correlation between plasma LDL-C level and mean methylation level (CpGZ%) at CpG10~12,CpG15,CpG20 locus. The difference was statistically significant (P0.05). 5. There was a significant positive linear correlation between age and average methylation level (CpGZ%) of LPL gene promoter (P0. 024) and a significant linear correlation at CpG9~16 locus (P0.05). 6. There was no significant difference in mean methylation (CpGZ%) level of LPL gene promoter between male and female in case group, control group and combined group (P0.05). The mean methylation level (CpGZ%) of LPL gene promoter was significantly different between male and control group (P0. 001). The mean methylation level (CpGZ%) of LPL gene promoter was significantly different between female and control group (P0. 024). Conclusion hypermethylation in the promoter region of 1.LPL gene is associated with cerebral infarction, low HDL-C level and high LDL-C level. 2.LPL promoter methylation may not be associated with infarct size and carotid atherosclerosis. There was no gender difference in methylation level in promoter region of 3.LPL gene, but it tended to increase with age.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3
【共引文献】
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2 徐程;丰蕾;杨帆;李彩霞;Q谜，
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