持续高频刺激改变短刺激产生的神经网络效应
发布时间：2018-12-18 20:39
【摘要】：不同时长的电脉冲高频刺激(high frequency stimulation,HFS)对于脑神经系统具有不同的作用.其中,数秒时长的短促HFS可通过"点燃"效应制作动物癫痫模型,也可以产生长时间保持的突触可塑性变化,而数分钟以上的长时HFS却可以安全地用于临床的深部脑刺激,治疗多种脑疾病.因此推测,持续的HFS可以改变短促刺激产生的效应.为了验证此推测,在大鼠海马CA1区的输入轴突纤维Schaffer侧支上,分别施加5 s和2 min两种时长的100 Hz HFS,并监测刺激结束后下游神经元群体对于单脉冲测试的响应电位,即群峰电位(population spike,PS).结果显示,5 s短HFS结束时会紧跟后放电痫样活动,并且,从测试脉冲诱发的PS幅值和潜伏期可见,短HFS诱导的兴奋性增强可以维持数十分钟.反之,2 min的长HFS结束时紧随之后的是数十秒无发放活动的静息期,而且,PS在数分钟内即恢复到HFS前的基线水平.这些结果表明,长时HFS的后期刺激可以改变前期短促刺激对于下游神经网络的作用,即消除短刺激可能产生的长时程兴奋效应.此发现对于深入了解高频刺激的作用机制、促进深部脑刺激的临床应用具有重要意义.
[Abstract]:High frequency electrical pulse stimulation (high frequency stimulation,HFS) with different duration has different effects on the cerebral nervous system. Among them, several seconds of short HFS can be used to make animal model of epilepsy by "kindling" effect, and can also produce long-term synaptic plasticity changes, while long-term HFS for more than a few minutes can be safely used in clinical deep brain stimulation. Treat a variety of brain diseases. Therefore, it is speculated that sustained HFS can alter the effects of short-term stimuli. To verify this hypothesis, the lateral branches of the input axon fiber Schaffer in the CA1 region of the hippocampus of the rat were treated with 100 Hz HFS, for 5 s and 2 min, respectively, and the response potentials of the downstream neuronal population to monopulse test were monitored after the end of the stimulation. Group peak potential (population spike,PS). The results showed that the epileptiform activity was closely followed at the end of 5 s short HFS, and the enhancement of excitability induced by short HFS could be maintained for tens of minutes from the amplitude and latency of PS induced by the test pulse. On the other hand, the long HFS of 2 min was followed by a few seconds of inactive activity at the end of the HFS period, and the PS returned to its baseline level before HFS within a few minutes. These results indicate that the late stimulation of long term HFS can change the effect of short stimulus on the downstream neural network, that is, to eliminate the long term excitatory effect of short stimulus. The findings are of great significance in understanding the mechanism of high frequency stimulation and promoting the clinical application of deep brain stimulation.
【作者单位】： 浙江大学生物医学工程与仪器科学学院生物医学工程教育部重点实验室;
【基金】：国家自然科学基金资助项目(30970753)~~
【分类号】：R741
本文编号：2386469
[Abstract]:High frequency electrical pulse stimulation (high frequency stimulation,HFS) with different duration has different effects on the cerebral nervous system. Among them, several seconds of short HFS can be used to make animal model of epilepsy by "kindling" effect, and can also produce long-term synaptic plasticity changes, while long-term HFS for more than a few minutes can be safely used in clinical deep brain stimulation. Treat a variety of brain diseases. Therefore, it is speculated that sustained HFS can alter the effects of short-term stimuli. To verify this hypothesis, the lateral branches of the input axon fiber Schaffer in the CA1 region of the hippocampus of the rat were treated with 100 Hz HFS, for 5 s and 2 min, respectively, and the response potentials of the downstream neuronal population to monopulse test were monitored after the end of the stimulation. Group peak potential (population spike,PS). The results showed that the epileptiform activity was closely followed at the end of 5 s short HFS, and the enhancement of excitability induced by short HFS could be maintained for tens of minutes from the amplitude and latency of PS induced by the test pulse. On the other hand, the long HFS of 2 min was followed by a few seconds of inactive activity at the end of the HFS period, and the PS returned to its baseline level before HFS within a few minutes. These results indicate that the late stimulation of long term HFS can change the effect of short stimulus on the downstream neural network, that is, to eliminate the long term excitatory effect of short stimulus. The findings are of great significance in understanding the mechanism of high frequency stimulation and promoting the clinical application of deep brain stimulation.
【作者单位】： 浙江大学生物医学工程与仪器科学学院生物医学工程教育部重点实验室;
【基金】：国家自然科学基金资助项目(30970753)~~
【分类号】：R741
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