亚硒酸钠对谷氨酸所致神经细胞损伤的影响及机制

发布时间：2018-12-29 09:03

【摘要】：目的观察亚硒酸钠对谷氨酸(Glu)所致神经细胞损伤的影响,并探讨其机制。方法将HT22神经细胞分成3组,对照组加入细胞培养液培养,Glu组加入6 mmol/L Glu和细胞培养液共同培养,亚硒酸钠组加入100nmol/L亚硒酸钠、6 mmol/L Glu和细胞培养液共同培养。MTT法检测各组6、10、24 h的细胞增殖抑制率;倒置显微镜观察细胞大体形态,透射电镜观察细胞超微结构;采用氧自由基(ROS)特异性标记探针DHE对各组细胞进行标记,用荧光电子显微镜观察DHE表达。结果 Glu组培养6 h时的细胞增殖抑制率为8.71%±0.009%,10、24h时细胞增殖抑制率较6 h时升高,24 h时最高;亚硒酸钠组各时点细胞增殖抑制率均较Glu组降低(P均0.05)。倒置显微镜下可见Glu组细胞数量较对照组减少,细胞皱缩、形态不规则,亚硒酸钠组细胞数量较Glu组数量增多;透射电镜下可见Glu组细胞凋亡数目较对照组增加,线粒体、内质网损伤加重,亚硒酸钠组细胞凋亡数量较Glu组减少。荧光电子显微镜观察显示,Glu组较对照组DHE染色的平均光密度增加,亚硒酸钠组DHE染色较Glu减轻(P均0.01)。结论硒能够减轻Glu对神经细胞的损伤作用,其机制可能与减少ROS产生有关。
[Abstract]:Objective to investigate the effect of sodium selenite on neuronal injury induced by glutamate (Glu) and its mechanism. Methods HT22 neurons were divided into three groups: the control group was cultured in cell culture medium, the Glu group was cultured in 6 mmol/L Glu and cell culture medium, and the sodium selenite group was added with 100nmol/L sodium selenite. (6) mmol/L Glu and cell culture medium were co-cultured. MTT assay was used to detect the inhibition rate of cell proliferation in each group for 24 h. The cell morphology was observed by inverted microscope and ultrastructure was observed by transmission electron microscope (TEM), and the expression of DHE was observed by fluorescence electron microscope (FEM) and oxygen free radical (ROS) specific probe DHE was used to label the cells in each group. Results the inhibition rate of cell proliferation in Glu group was 8.71% 卤0.009% at 6 h and increased at 10h and the highest at 24 h. The inhibition rate of cell proliferation in sodium selenite group was lower than that in Glu group at all time points (P 0.05). Under inverted microscope, the number of cells in Glu group was less than that in control group, cell shrinkage and irregular morphology were observed, and the number of cells in sodium selenite group was higher than that in Glu group. Transmission electron microscope showed that the number of apoptosis in Glu group was higher than that in control group, the damage of mitochondria and endoplasmic reticulum was aggravated, and the number of apoptosis in sodium selenite group was lower than that in Glu group. Fluorescence electron microscopy showed that the average optical density of DHE staining in Glu group was higher than that in control group, while DHE staining in sodium selenite group was less than that in Glu group (P0.01). Conclusion selenium can attenuate the injury of Glu to nerve cells, and its mechanism may be related to the reduction of ROS production.
【作者单位】：宁夏医科大学;银川市妇幼保健院;陕西省中医院;
【基金】：国家自然科学基金地区项目(81560501) 宁夏高等学校科学研究项目(NGY2015068)
【分类号】：R741

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