河北某地区ORP9基因多态性与脑梗死的相关性研究
发布时间：2019-01-01 13:35
【摘要】：目的将氧化固醇相关蛋白-9(Oxysterol·Binding Protein-related Protein-9,ORP-9)作为脑梗死的候选基因,检测其在河北汉族人群中的分布频率,分析ORP-9的基因多态性与脑梗死的关系,并分析其与脑梗死的关键性危险因素-动脉粥样硬化(Atheroselerosis,AS)、血脂、血压及血糖的关系。方法选华北理工大学附属医院收治的籍贯来自河北地区无血缘关系的脑梗死患者174人作为脑梗死组,另选年龄和性别相仿的河北籍贯的无血缘关系的来自同一医院无脑梗死患者或者同期到华北理工大学附属医院体检中心的体检健康者作为对照组,共117例。于清晨抽取研究对象的血液样本5ml,并追踪其病史及相关检验、检查,与对照组进行病例对照研究。把抽的血液样本使用全血提取试剂盒提取DNA,然后做PCR检测,把经过PCR扩增的产物进行限制性片段长度多态性分析(Restriction Fragment Length Polymorphism,RFLP),用来检测ORP-9的两个位点rs856600及rs768529的单核苷酸多态性,检测其基因型及等位基因的分布。使用t检验、卡方检验及Logistic回归模型处理统计数据,并且使用SPSS l 7.0统计软件对得到的统计数据进行分析。结果1在脑梗死组与对照组的基本临床资料的比较中,在很多方面,如年龄、性别、吸烟史及饮酒史上,两组间无统计学意义(P0.05);与对照组相比,脑梗死组在体重指数、糖尿病史、高血压病史、收缩压、舒张压、空腹血糖、TG、TC及LDL比例大(P0.05);而脑梗死的HDL比对照组的人数少(P0.05)。2rs856600与rs768529的基因型与等位基因频率在脑梗死与对照组比较中均无统计学意义(P0.05);它们的Logistic回归分析结果,所有基因型及等位基因频率和脑梗死的发病率无相关性(P0.05)。3有高血压病史的脑梗死组(HBP+CI)与无高血压病史的脑梗死组(非HBP+CI)与相比,ORP-9两个位点的基因型和等位基因频率分布无统计学意义(P0.05);有糖尿病史的脑梗死组(DM+CI组)与无糖尿病史的脑梗死组(非DM+CI组)相比,ORP-9两个位点的基因型和等位基因频率的分布也无统计学意义(P0.05)。4在rs856600的C与T(CT+TT)和rs768529的A(AA+AT)与T的人群中的比较,血压、空腹血糖、及TG、TC、HDL、LDL水平无统计学意义(P0.05)。5 rs856600和rs768529的基因型及等位基因频率在WAS合并脑梗死、WAS的对照组中、AS合并脑梗死和AS的对照组中差异无统计学意义(P0.05);在VP合并脑梗死和SP合并脑梗死中,rs856600的基因型及等位基因频率分布差异无统计学意义(P0.05),rs768529的基因型及等位基因频率分布在VP合并脑梗死和SP合并脑梗死中差异有统计学意义(P0.05)。结论ORP-9的两个位点的基因多态性跟河北地区人群脑梗死及影响脑梗死的危险因素如高血压、高血糖及TG、TC、HDL、LDL等血脂因素可能不相关。rs856600的基因型及等位基因频率跟AS及VP和SP不相关,rs768529的T等位基因可能是河北地区汉族人群动脉粥样硬化的易损斑块的一个危险的因素。
[Abstract]:Objective to study the relationship between ORP-9 gene polymorphism and cerebral infarction by using steroid associated protein 9 (Oxysterol Binding Protein-related Protein-9,ORP-9 as a candidate gene for cerebral infarction, and to detect its distribution frequency in Hebei Han population. The relationship between Atherosclerosis (Atheroselerosis,AS), blood lipid, blood pressure and blood glucose was analyzed. Methods 174 patients with cerebral infarction from Hebei province were selected as cerebral infarction group, who were treated in affiliated Hospital of North China University of Science and Technology. A total of 117 healthy subjects from the same hospital without cerebral infarction or from the physical examination center of affiliated hospital of North China University of Technology were selected as the control group. The blood samples of the subjects were collected in the morning, and their medical history and related tests were followed up. The case control study was carried out with the control group. The extracted blood samples were extracted by whole blood extraction kit (DNA,) and then detected by PCR. The products amplified by PCR were analyzed by restriction fragment length polymorphism (Restriction Fragment Length Polymorphism,RFLP). Single nucleotide polymorphisms (SNP) of rs856600 and rs768529 at the two loci of ORP-9 were detected and their genotypes and alleles were detected. T test, chi-square test and Logistic regression model were used to process the statistical data, and the statistical data were analyzed by SPSS l 7.0 software. Results 1 in the comparison of the basic clinical data between the cerebral infarction group and the control group, there was no statistical significance between the two groups in many aspects, such as age, sex, smoking history and drinking history (P0.05). Compared with the control group, the proportion of body mass index, history of diabetes, history of hypertension, systolic blood pressure, diastolic blood pressure, fasting blood glucose, TG,TC and LDL were higher in cerebral infarction group (P0.05). However, the number of HDL in cerebral infarction was lower than that in control group (P0.05). The genotype and allele frequency of 2rs856600 and rs768529 had no significant difference between cerebral infarction and control group (P0.05). Their Logistic regression results, There was no correlation between the frequency of all genotypes and alleles and the incidence of cerebral infarction (P0.05). 3 (HBP CI) in cerebral infarction group with history of hypertension and cerebral infarction without hypertension history (non-HBP CI) compared with non-hypertensive group), The frequency distribution of genotype and allele of two ORP-9 loci had no statistical significance (P0.05). The cerebral infarction group with diabetes history (DM CI group) was compared with the cerebral infarction group (non-diabetic DM CI group) with no diabetes history. The distribution of genotype and allele frequencies at ORP-9 loci was also not statistically significant (P0.05). 4 comparison of blood pressure, fasting blood glucose, and TG,TC, between C and T (CT TT) of rs856600 and A (AA AT) and T of rs768529 There was no significant difference in HDL,LDL level (P0.05). 5 the genotype and allele frequency of rs856600 and rs768529 were not significantly different in WAS with cerebral infarction, AS with cerebral infarction and AS (P0.05). There was no significant difference in the frequency distribution of rs856600 genotype and allele between VP with cerebral infarction and SP with cerebral infarction (P0.05). The genotypes and allelic frequencies of rs768529 were significantly different between VP with cerebral infarction and SP with cerebral infarction (P0.05). Conclusion the polymorphism of two loci of ORP-9 is associated with cerebral infarction and risk factors such as hypertension, hyperglycemia and TG,TC,HDL, in Hebei population. The genotype and allele frequency of rs856600 may not be associated with AS, VP and SP. The T allele of rs768529 may be a risk factor for atherosclerotic plaque in Hebei Han population.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.33
本文编号：2397640
[Abstract]:Objective to study the relationship between ORP-9 gene polymorphism and cerebral infarction by using steroid associated protein 9 (Oxysterol Binding Protein-related Protein-9,ORP-9 as a candidate gene for cerebral infarction, and to detect its distribution frequency in Hebei Han population. The relationship between Atherosclerosis (Atheroselerosis,AS), blood lipid, blood pressure and blood glucose was analyzed. Methods 174 patients with cerebral infarction from Hebei province were selected as cerebral infarction group, who were treated in affiliated Hospital of North China University of Science and Technology. A total of 117 healthy subjects from the same hospital without cerebral infarction or from the physical examination center of affiliated hospital of North China University of Technology were selected as the control group. The blood samples of the subjects were collected in the morning, and their medical history and related tests were followed up. The case control study was carried out with the control group. The extracted blood samples were extracted by whole blood extraction kit (DNA,) and then detected by PCR. The products amplified by PCR were analyzed by restriction fragment length polymorphism (Restriction Fragment Length Polymorphism,RFLP). Single nucleotide polymorphisms (SNP) of rs856600 and rs768529 at the two loci of ORP-9 were detected and their genotypes and alleles were detected. T test, chi-square test and Logistic regression model were used to process the statistical data, and the statistical data were analyzed by SPSS l 7.0 software. Results 1 in the comparison of the basic clinical data between the cerebral infarction group and the control group, there was no statistical significance between the two groups in many aspects, such as age, sex, smoking history and drinking history (P0.05). Compared with the control group, the proportion of body mass index, history of diabetes, history of hypertension, systolic blood pressure, diastolic blood pressure, fasting blood glucose, TG,TC and LDL were higher in cerebral infarction group (P0.05). However, the number of HDL in cerebral infarction was lower than that in control group (P0.05). The genotype and allele frequency of 2rs856600 and rs768529 had no significant difference between cerebral infarction and control group (P0.05). Their Logistic regression results, There was no correlation between the frequency of all genotypes and alleles and the incidence of cerebral infarction (P0.05). 3 (HBP CI) in cerebral infarction group with history of hypertension and cerebral infarction without hypertension history (non-HBP CI) compared with non-hypertensive group), The frequency distribution of genotype and allele of two ORP-9 loci had no statistical significance (P0.05). The cerebral infarction group with diabetes history (DM CI group) was compared with the cerebral infarction group (non-diabetic DM CI group) with no diabetes history. The distribution of genotype and allele frequencies at ORP-9 loci was also not statistically significant (P0.05). 4 comparison of blood pressure, fasting blood glucose, and TG,TC, between C and T (CT TT) of rs856600 and A (AA AT) and T of rs768529 There was no significant difference in HDL,LDL level (P0.05). 5 the genotype and allele frequency of rs856600 and rs768529 were not significantly different in WAS with cerebral infarction, AS with cerebral infarction and AS (P0.05). There was no significant difference in the frequency distribution of rs856600 genotype and allele between VP with cerebral infarction and SP with cerebral infarction (P0.05). The genotypes and allelic frequencies of rs768529 were significantly different between VP with cerebral infarction and SP with cerebral infarction (P0.05). Conclusion the polymorphism of two loci of ORP-9 is associated with cerebral infarction and risk factors such as hypertension, hyperglycemia and TG,TC,HDL, in Hebei population. The genotype and allele frequency of rs856600 may not be associated with AS, VP and SP. The T allele of rs768529 may be a risk factor for atherosclerotic plaque in Hebei Han population.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.33
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