脑血流监测对大鼠脑缺血模型制备的评价作用
发布时间：2019-01-05 06:18
【摘要】：目的 探讨脑血流监测对线栓法制备大鼠大脑中动脉阻塞(MCAO)局灶性脑缺血模型的评价作用。方法 分别将线栓插入30只SPF级Wistar Han大鼠颈内动脉颅内段(16.0±0.5)、(18.0±0.5)和(20.0±0.5)mm,制备3种局灶性脑缺血模型(各10只),然后将所有实验大鼠依据颅底有无血凝块及2,3,5氯化三苯基四氮(TTC)染色后大脑中动脉供血区有无梗死灶分为不全阻塞组、完全阻塞组及过深阻塞组,对阻塞颈内动脉颅内段前后及拔出线栓再灌注后每只大鼠大脑中动脉供血区脑皮质的血流量以激光多普勒法进行监测记录并进行统计学分析。大脑中动脉供血区脑皮质的血流量以相对流量单位PU值表示;阻塞后及再灌注后的脑皮质血流量变化以与阻塞前脑皮质血流量的百分比表示。结果 模型制作过程中,1只大鼠死亡;不全阻塞组9只,完全阻塞组15只,过深阻塞组5只。不全阻塞组8只大鼠线栓插入深度在(16.0±0.5)mm,不能完全阻止大脑前动脉向大脑中动脉的血流,缺血6 h后大鼠Longa评分0~1分;颅底动脉环周围无血凝块,经TTC染色后无梗死灶。完全阻塞组9只大鼠线栓插入深度在(18.0±0.5)mm,大脑前动脉的血流被完全阻断,缺血6 h后大鼠Longa评分2~3分;颅底动脉环周围无血凝块而TTC染色提示存在大脑中动脉供血区的梗死灶。过深阻塞组5只大鼠线栓插入深度在(20.0±0.5)mm,可完全阻断大脑前动脉血流,缺血6 h后大鼠Longa评分3~4分;解剖可见颅底血凝块,TTC染色后可见中动脉供血区梗死灶。插入线栓后,不全阻塞组、完全阻塞组和过深阻塞组大鼠脑皮质血流量均较阻塞前下降(分别为94±17比256±36、43±9比286±44、44±6比294±46,均P0.05),组间差异有统计学意义(F=56.57,P0.01),完全阻塞组和过深阻塞组血流量明显低于不全阻塞组(均P0.05),完全阻塞组与过深阻塞组间差异无统计学意义(P0.05);3组阻塞后与阻塞前脑皮质血流量的百分比分别为(36.93±0.06)%、(15.09±0.02)%、(15.52±0.04)%,组间差异有统计学意义(F=39.14,P0.01)。再灌注后,不全阻塞组、完全阻塞组和过深阻塞组脑皮质血流量(分别为213±31、147±17、96±14)均较阻塞后有明显回升(均P0.05),组间差异有统计学意义(F=50.05,P0.01),过深阻塞组脑皮质血流量明显低于完全阻塞组(P0.05);3组再灌注后与阻塞前脑皮质血流量水平百分比分别为(83.10±0.02)%、(51.83±0.05)%、(33.49±0.09)%,差异有统计学意义(F=93.23,P0.01)。结论 以激光多普勒对脑血流进行监测,可作为判断线栓法制备大鼠MCAO脑缺血模型成功与否的一种实时、便捷、微创、客观可靠的评价手段。
[Abstract]:Objective to evaluate the effect of cerebral blood flow monitoring on focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO) in rats. Methods three kinds of focal cerebral ischemia models were established in 30 SPF grade Wistar Han rats by inserting them into the intracranial segment of the internal carotid artery (16.0 卤0.5), (18.0 卤0.5) and (20.0 卤0.5) mm, respectively. Then all the experimental rats were divided into three groups: incomplete occlusion group, complete occlusion group and over-deep occlusion group, according to the presence of blood clots in the skull base and the presence or absence of infarction in the blood supply area of the middle cerebral artery (MCAA) after the staining of triphenyl tetrazolium chloride (TTC). The blood flow in the cerebral cortex of the middle cerebral artery in each rat was monitored and analyzed by laser Doppler method before and after the occlusion of the internal carotid artery and the reperfusion of the extracellular thrombus. The cortical blood flow in the middle cerebral artery (MCA) region was expressed as relative flow unit (PU), and the cortical blood flow after occlusion and reperfusion was expressed as the percentage of cerebral cortical blood flow (CBF) after occlusion and reperfusion. Results during the course of making the model, one rat died, 9 rats were in incomplete occlusion group, 15 rats in complete occlusion group and 5 rats in over-deep occlusion group. In the incomplete occlusion group, the insertion depth (16.0 卤0.5) mm, could not completely prevent the anterior cerebral artery from flowing to the middle cerebral artery, and the Longa score of the rats was 0-1 after 6 hours of ischemia. There was no blood clot around the basilar artery ring and no infarct after TTC staining. In the complete occlusion group, the blood flow of the anterior cerebral artery was completely blocked at the depth of (18.0 卤0.5) mm, and the Longa score of the rats was 2 ~ 3 points after 6 hours of ischemia. There was no blood clot around the basilar artery ring and TTC staining suggested that there was a infarct in the middle cerebral artery. At the depth of (20.0 卤0.5) mm, the anterior cerebral artery blood flow could be completely blocked in the overdeep occlusion group. The Longa score of the rats was 3 ~ 4 points after 6 hours of ischemia, and the blood clot in the skull base could be seen at the anatomic level, and the infarcted area of the blood supply area of the middle artery could be seen after TTC staining. The cerebral cortical blood flow decreased significantly in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (94 卤17 vs 256 卤36 卤36 卤43 卤9 vs 286 卤44 卤6 vs 294 卤46, respectively, P0.05), and the difference between the two groups was statistically significant (F 56.57 P0.01), and the cerebral cortical blood flow decreased significantly in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (94 卤17 vs 256 卤36, 43 卤9 vs 286 卤44 卤6 vs 294 卤46, respectively, P0.05). The blood flow in the complete occlusion group and the deep occlusion group was significantly lower than that in the incomplete occlusion group (P0.05), but there was no significant difference between the complete occlusion group and the over-deep occlusion group (P0.05). The percentage of cerebral cortical blood flow after occlusion was (36.93 卤0.06)%, (15.09 卤0.02)%, (15.52 卤0.04)%, respectively. After reperfusion, the cerebral cortical blood flow increased significantly (213 卤31147 卤1796 卤14) in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (P0.05), and the difference between the two groups was statistically significant (F0. 05 卤0. 01). The cerebral cortical blood flow in the deep occlusion group was significantly lower than that in the complete occlusion group (P0.05). The percentage of cortical blood flow after reperfusion was (83.10 卤0.02)%, (51.83 卤0.05)%, (33.49 卤0.09)%, respectively. The difference was statistically significant (F _ (93.23) P _ (0.01). Conclusion the monitoring of cerebral blood flow by laser Doppler can be used as a real-time, convenient, minimally invasive, objective and reliable method to evaluate the success of MCAO cerebral ischemia in rats.
【作者单位】： 新乡医学院第一附属医院神经外科;
【分类号】：R-332;R743.3
[Abstract]:Objective to evaluate the effect of cerebral blood flow monitoring on focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO) in rats. Methods three kinds of focal cerebral ischemia models were established in 30 SPF grade Wistar Han rats by inserting them into the intracranial segment of the internal carotid artery (16.0 卤0.5), (18.0 卤0.5) and (20.0 卤0.5) mm, respectively. Then all the experimental rats were divided into three groups: incomplete occlusion group, complete occlusion group and over-deep occlusion group, according to the presence of blood clots in the skull base and the presence or absence of infarction in the blood supply area of the middle cerebral artery (MCAA) after the staining of triphenyl tetrazolium chloride (TTC). The blood flow in the cerebral cortex of the middle cerebral artery in each rat was monitored and analyzed by laser Doppler method before and after the occlusion of the internal carotid artery and the reperfusion of the extracellular thrombus. The cortical blood flow in the middle cerebral artery (MCA) region was expressed as relative flow unit (PU), and the cortical blood flow after occlusion and reperfusion was expressed as the percentage of cerebral cortical blood flow (CBF) after occlusion and reperfusion. Results during the course of making the model, one rat died, 9 rats were in incomplete occlusion group, 15 rats in complete occlusion group and 5 rats in over-deep occlusion group. In the incomplete occlusion group, the insertion depth (16.0 卤0.5) mm, could not completely prevent the anterior cerebral artery from flowing to the middle cerebral artery, and the Longa score of the rats was 0-1 after 6 hours of ischemia. There was no blood clot around the basilar artery ring and no infarct after TTC staining. In the complete occlusion group, the blood flow of the anterior cerebral artery was completely blocked at the depth of (18.0 卤0.5) mm, and the Longa score of the rats was 2 ~ 3 points after 6 hours of ischemia. There was no blood clot around the basilar artery ring and TTC staining suggested that there was a infarct in the middle cerebral artery. At the depth of (20.0 卤0.5) mm, the anterior cerebral artery blood flow could be completely blocked in the overdeep occlusion group. The Longa score of the rats was 3 ~ 4 points after 6 hours of ischemia, and the blood clot in the skull base could be seen at the anatomic level, and the infarcted area of the blood supply area of the middle artery could be seen after TTC staining. The cerebral cortical blood flow decreased significantly in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (94 卤17 vs 256 卤36 卤36 卤43 卤9 vs 286 卤44 卤6 vs 294 卤46, respectively, P0.05), and the difference between the two groups was statistically significant (F 56.57 P0.01), and the cerebral cortical blood flow decreased significantly in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (94 卤17 vs 256 卤36, 43 卤9 vs 286 卤44 卤6 vs 294 卤46, respectively, P0.05). The blood flow in the complete occlusion group and the deep occlusion group was significantly lower than that in the incomplete occlusion group (P0.05), but there was no significant difference between the complete occlusion group and the over-deep occlusion group (P0.05). The percentage of cerebral cortical blood flow after occlusion was (36.93 卤0.06)%, (15.09 卤0.02)%, (15.52 卤0.04)%, respectively. After reperfusion, the cerebral cortical blood flow increased significantly (213 卤31147 卤1796 卤14) in the incomplete occlusion group, complete occlusion group and over-deep occlusion group (P0.05), and the difference between the two groups was statistically significant (F0. 05 卤0. 01). The cerebral cortical blood flow in the deep occlusion group was significantly lower than that in the complete occlusion group (P0.05). The percentage of cortical blood flow after reperfusion was (83.10 卤0.02)%, (51.83 卤0.05)%, (33.49 卤0.09)%, respectively. The difference was statistically significant (F _ (93.23) P _ (0.01). Conclusion the monitoring of cerebral blood flow by laser Doppler can be used as a real-time, convenient, minimally invasive, objective and reliable method to evaluate the success of MCAO cerebral ischemia in rats.
【作者单位】： 新乡医学院第一附属医院神经外科;
【分类号】：R-332;R743.3
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