炎性神经系统疾病患者脑脊液博尔纳病病毒CIC及抗体的检测

发布时间：2019-01-30 10:53

【摘要】：目的：探讨贵州省贵阳、遵义、都匀等地炎性神经系统疾病中博尔纳病病毒(Bornadisease virus，BDV)感染情况和主要感染方式，以及病毒性脑炎BDV CIC阳性患者的临床特征。方法：采用酶联免疫吸附试验（ELISA）检测84例炎性神经系统疾病患者，包括69例病毒性脑炎（VE）患者，9例格林巴利综合征（GBS）患者，6例多发性硬化（MS）患者，以及45例对照组(非炎症性神经系统疾病患者)脑脊液（CSF）中BDV循环免疫复合物（CIC）及抗体，对脑脊液BDV CIC阳性患者取其外周血同期行血液BDV CIC检测。结果：84例炎性神经系统疾病患者CSF中BDV CIC总阳性率为15.40%(13/84)，其中VE阳性率14.40％（10/69），GBS阳性率11.10％(1/9)，，MS阳性率33.30％(2/6)，对照组阳性率2.22％(1/45)，实验组总体阳性率明显高于对照组（P＜0.05），两组比较差异具有统计学意义。CSF抗体总体阳性率1.20％(1/84)，对照组抗体阳性率为0，两组比较差异无统计学意义（P＞0.05）。13例脑脊液BDV CIC阳性患者，外周血BDV CIC阳性者10例，阳性程度高于脑脊液。7例脑脊液BDV CIC阳性病毒性脑炎患者主要以精神行为异常为特征，患者有密切动物接触史。结论：贵州省贵阳、遵义、都匀等地在炎性神经系统疾病患者中存在BDV感染；BDV特异性CIC阳性VE患者主要以精神行为异常为临床特征；BDV感染方式以隐性感染为主，隐性感染的患者可能超出显性感染者的比例；BDV与人类的密切关系可能较高。
[Abstract]:Objective: to investigate the infection of Borna disease virus (Bornadisease virus,BDV) in inflammatory nervous system diseases in Guiyang, Zunyi and Duyun, Guizhou Province, and the clinical characteristics of BDV CIC positive patients with viral encephalitis. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to detect 84 patients with inflammatory nervous system diseases, including 69 patients with viral encephalitis (VE), 9 patients with Guillain-Barre syndrome (GBS) and 6 patients with multiple sclerosis (MS). BDV circulating immune complex (CIC) and antibody in cerebrospinal fluid (CSF) were collected from 45 patients with BDV CIC positive cerebrospinal fluid (BDV CIC) in cerebrospinal fluid (CSF). Results: the total positive rate of BDV CIC in CSF of 84 patients with inflammatory nervous system diseases was 15.40% (13 / 84), and the positive rate of VE was 14.40% (10 / 69), GBS) 11.10% (1 / 9). The positive rate of MS was 33.30% (2 / 6) and the positive rate of control group was 2.22% (1 / 45). The total positive rate of experimental group was significantly higher than that of control group (P < 0. 05). The total positive rate of CSF antibody was 1.20% (1 / 84), while the positive rate of control group was 0. There was no significant difference between the two groups (P > 0. 05). The positive degree of BDV CIC in peripheral blood was higher in 10 cases than that in cerebrospinal fluid (CSF). 7 cases of BDV CIC positive viral encephalitis were mainly characterized by abnormal mental behavior and had close animal contact history. Conclusion: in Guiyang, Zunyi and Duyun of Guizhou Province, there is BDV infection in patients with inflammatory nervous system diseases, and the main clinical features of VE patients with BDV specific CIC are abnormal mental behavior. Recessive infection is the main mode of BDV infection, and the proportion of patients with recessive infection may be higher than that of dominant infection, and the close relationship between BDV and human may be higher.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R741

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