一锅法合成近红外量子点用于胶质瘤荧光显像与光动力治疗
发布时间：2019-03-19 22:09
【摘要】：目的:合成近红外量子点并验证其荧光成像和光动力效应。方法:应用一锅法水相合成近红外量子点CdTe,经表征检测后连接RGD多肽,再用激光共聚焦显微镜观察其荧光成像效果。随后,将3~24μg/mL的CdTe-RGD分别加入U251和3T3细胞,避光培养24 h间,观察量子点的细胞毒性。最后用强度为30 mW/cm~2,波长为632.8 nm的激光照射量子点处理后的细胞5~60 min,观察其光动力效应。结果:合成的CdTe具有良好的晶体结构和分散性,其直径为3.75 nm,发射波长700 nm,荧光产率20%。经量子点处理后,U251细胞可荧光显像而3T3细胞则不能。暗环境下,不同浓度CdTe-RGD处理过的U251和3T3细胞存活率都在85%以上。激光照射后,随光照时间延长和量子点浓度的增加,U251细胞存活逐渐下降至(37±1.6)%。而3T3细胞组的存活率则无明显变化。结论:一锅法水相合成的CdTe-RGD具有良好的理化性质且细胞毒性低,在生物成像和光动力治疗方面具有应用前景。
[Abstract]:Aim: to synthesize near infrared quantum dots and verify their fluorescence imaging and photodynamic effects. Methods: the near infrared quantum dots (CdTe,) were synthesized by one-pot method in aqueous phase, and then the RGD polypeptide was ligated. Then the fluorescence imaging effect was observed by confocal laser microscope (LSCM). Then, the CdTe-RGD of 3 ~ 24 渭 g / mL was added to U251 and 3T3 cells, respectively, and the cytotoxicity of QDs was observed for 24 h without light. Finally, the photodynamic effects of quantum dots treated with 30 mW/cm~2, laser at 632.8 nm were observed at 5 / 60 min,. Results: the synthesized CdTe has good crystal structure and dispersibility. Its diameter is 3.75 nm, emission wavelength 700 nm, fluorescence yield is 20%. After treatment with quantum dots, U251 cells could be visualized by fluorescence, but 3T3 cells could not. In dark environment, the survival rates of U251 and 3T3 cells treated with different concentrations of CdTe-RGD were above 85%. After laser irradiation, the survival of U251 cells decreased to (37 卤1.6)% with the prolongation of irradiation time and the increase of quantum dot concentration. However, the survival rate of 3T3 cells did not change significantly. Conclusion: CdTe-RGD synthesized by one-pot aqueous phase has good physicochemical properties and low cytotoxicity. It has a promising future in bioimaging and photodynamic therapy.
【作者单位】： 上海交通大学医学院附属新华医院神经外科;上海交通大学纳米生物医学工程研究所薄膜与微细技术教育部重点实验室;
【基金】：国家自然科学基金项目(编号:81471317) 上海市科学技术委员会引导类重点项目(编号:124119a0800)
【分类号】：R739.41
本文编号：2443955
[Abstract]:Aim: to synthesize near infrared quantum dots and verify their fluorescence imaging and photodynamic effects. Methods: the near infrared quantum dots (CdTe,) were synthesized by one-pot method in aqueous phase, and then the RGD polypeptide was ligated. Then the fluorescence imaging effect was observed by confocal laser microscope (LSCM). Then, the CdTe-RGD of 3 ~ 24 渭 g / mL was added to U251 and 3T3 cells, respectively, and the cytotoxicity of QDs was observed for 24 h without light. Finally, the photodynamic effects of quantum dots treated with 30 mW/cm~2, laser at 632.8 nm were observed at 5 / 60 min,. Results: the synthesized CdTe has good crystal structure and dispersibility. Its diameter is 3.75 nm, emission wavelength 700 nm, fluorescence yield is 20%. After treatment with quantum dots, U251 cells could be visualized by fluorescence, but 3T3 cells could not. In dark environment, the survival rates of U251 and 3T3 cells treated with different concentrations of CdTe-RGD were above 85%. After laser irradiation, the survival of U251 cells decreased to (37 卤1.6)% with the prolongation of irradiation time and the increase of quantum dot concentration. However, the survival rate of 3T3 cells did not change significantly. Conclusion: CdTe-RGD synthesized by one-pot aqueous phase has good physicochemical properties and low cytotoxicity. It has a promising future in bioimaging and photodynamic therapy.
【作者单位】： 上海交通大学医学院附属新华医院神经外科;上海交通大学纳米生物医学工程研究所薄膜与微细技术教育部重点实验室;
【基金】：国家自然科学基金项目(编号:81471317) 上海市科学技术委员会引导类重点项目(编号:124119a0800)
【分类号】：R739.41
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