BQ-123通过mTOR-自噬通路对蛛网膜下腔出血大鼠神经细胞凋亡的影响

发布时间：2019-04-03 14:55

【摘要】：目的:探讨BQ-123对蛛网膜下腔出血(SAH)的治疗作用及其机制。方法:120只雄性SD大鼠,分为假手术(Sham)组、SAH组、雷帕霉素组、BQ-123干预组。二次注血法制作SAH大鼠模型;镜下观察海马区组织形态结构变化;免疫组化法和RT-PCR法检测海马区雷帕霉素靶蛋白(m TOR)、自噬相关基因Beclin-1和微管相关蛋白1轻链(LC3)-Ⅱ的表达;TUNEL法检测细胞凋亡情况。结果:SAH组海马区可见神经细胞变性水肿和死亡;血管内皮细胞肿胀、凝集,管腔受压、狭窄明显,管壁迂曲不平、缝隙连接断裂、基膜松散、模糊甚至分离。雷帕霉素组大部分细胞排列整齐、细胞结构完整;血管内皮细胞肿胀、凝集,管腔受压、狭窄程度减轻,管壁迂曲不平;BQ-123干预组结构完整神经细胞显著增多;血管内皮细胞凝集已少见,膜结构完整、清晰,基膜厚薄均匀。与SAH组比较,雷帕霉素组和BQ-123干预组海马区m TOR表达降低,Beclin-1和LC3-Ⅱ表达增加,凋亡神经细胞数量减少(P0.05)。结论:BQ-123可抑制SAH大鼠神经细胞凋亡,其机制与调控m TOR-自噬信号途径有关。
[Abstract]:Objective: to investigate the therapeutic effect and mechanism of BQ-123 on subarachnoid hemorrhage (SAH) with (SAH). Methods: 120 male SD rats were divided into sham-operated (Sham) group, SAH group, rapamycin group and BQ-123 intervention group. The rat model of SAH was made by twice blood injection, and the morphological changes of hippocampus were observed under microscope. The expression of (m TOR), autophagy-associated gene Beclin-1 and microtubule-associated protein 1 light chain (LC3-鈪，

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