高渗氯化钠羟乙基淀粉40对大鼠脑缺血再灌注损伤后MMP-9和层粘连蛋白表达的影响

发布时间：2019-04-25 18:00

【摘要】：目的探讨高渗氯化钠羟乙基淀粉40(HSH)对大鼠脑缺血-再灌注(I/R)损伤后神经功能行为学评分,脑水肿情况,脑梗死体积以及脑组织中基质金属蛋白酶-9(MMP-9)和层粘连蛋白(Laminin)表达的影响。方法48只雄性SD大鼠随机分为假手术组、模型组、实验1组、实验2组。大鼠局灶性脑I/R损伤模型制备成功后120 min,假手术组和模型组静脉输注0.9%生理盐水,实验1组和2组分别静脉输注HSH 4 ml/kg和8 ml/kg。再灌注24 h后,行神经功能行为学评分,观察脑水肿情况,TTC染色测算脑梗死体积,用免疫印迹方法检测缺血区脑组织MMP-9和Laminin的表达。结果与假手术组比较,模型组神经功能行为学评分升高(P0.001);脑水肿加重;脑梗死体积增加(P0.001);MMP-9蛋白表达升高(P0.05);Laminin蛋白表达降低(P0.01)。与模型组比较,实验1组和2组神经功能行为学评分降低(P0.001);脑水肿缓解;脑梗死体积减少(P0.001);实验1组MMP-9蛋白表达减少(P0.01),Laminin蛋白表达升高(P0.05);实验2组MMP-9蛋白和Laminin蛋白表达均无明显变化。结论用HSH治疗大鼠脑I/R损伤后,大鼠神经功能行为学评分改善,脑水肿减轻,脑梗死体积减小,血脑屏障通透性降低,其机制可能与降低MMP-9和增加Laminin蛋白的表达有关。且保护作用以4 ml/kg剂量效果更佳。
[Abstract]:Objective to investigate the effects of hypertonic sodium chloride hydroxyethyl starch (40 (HSH) on neurobehavioral score and brain edema after cerebral ischemia-reperfusion (I / R) injury in rats. Effect of infarct volume and expression of matrix metalloproteinase-9 (MMP-9) and laminin (Laminin) in brain tissue. Methods 48 male SD rats were randomly divided into sham operation group, model group, experiment 1 group and experiment 2 group. HSH 4 ml/kg and 8 ml/kg. were injected intravenously into the sham operation group and model group at 120 min, after the establishment of focal cerebral I / R injury model in rats. HSH 4 ml/kg and 8 ml/kg. were injected intravenously in group 1 and group 2, respectively. 24 hours after reperfusion, neurobehavioral scores were performed to observe cerebral edema, TTC staining was used to measure the volume of cerebral infarction, and Western blot was used to detect the expression of MMP-9 and Laminin in ischemic brain tissue. Results compared with the sham-operated group, the neurobehavioral score of the model group increased (P0.001), the cerebral edema increased, the infarct volume increased (P0.001), the expression of MMP-9 protein increased (P0.05) the expression of); Laminin protein decreased (P0.01). Compared with the model group, the neurobehavioral scores of experimental group 1 and group 2 decreased (P0.001), cerebral edema alleviated, cerebral infarction volume decreased (P0.001), and cerebral infarction volume decreased (P0.001). The expression of MMP-9 protein was decreased in experiment 1 group (P0.01), Laminin protein expression was increased (P0.05), but there was no significant change in MMP-9 protein and Laminin protein expression in experiment 2 group. Conclusion after treatment with HSH, the neurobehavioral scores were improved, cerebral edema, cerebral infarction volume and blood-brain barrier permeability were decreased in rats. The mechanism may be related to the decrease of MMP-9 and the increase of Laminin protein expression. [WT5 "HZ] conclusion [WT5" BZ] [WT5 "BZ] The protective effect was better at the dose of 4 ml/kg.
【作者单位】：赣南医学院研究生学院;赣南医学院;赣南医学院生理学教研室;赣南医学院疼痛医学研究所;赣南医学院第一附属医院麻醉科;
【基金】：江西省教育厅科学技术研究项目(No.GJJ12548) 江西省自然科学基金项目(No.20142BAB205020) 赣南医学院研究生创新专项资金项目(No.YC2015-S408)
【分类号】：R743

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