猪脑死亡对血清ET-1及TNF-α、IL-1β、IL-6表达影响的初步研究

发布时间：2019-05-05 19:08

【摘要】：目的:探讨"渐进式"颅内加压法诱导猪脑死亡对血清中ET-1、TNF-α、IL-1β及IL-6表达的影响。方法:雄性长白猪12头,随机分为两组,对照组:颅内置入颅内压探头及水囊导管,但不进行颅内加压;脑死亡组:颅内置入颅内压探头及水囊导管,根据颅内压和平均动脉压的关系,调整加压强度建立脑死亡模型,并通过呼吸、循环维持脑死亡状态12 h,并检测血清ET-1、TNF-α、IL-1β及IL-6水平。结果:脑死亡组ET-1水平从脑死亡0 h即逐渐升高,TNF-α、IL-1β、IL-6从脑死亡后6 h开始升高,上述因子水平与对照组比较,差异均有统计学意义(P0.05)。结论:脑死亡时TNF-α、IL-1β、IL-6等炎症因子升高相对迟缓,而ET-1水平发生变化较早,灵敏度高,这可能与其作为前炎症介质有关。
[Abstract]:Aim: to investigate the effects of progressive intracranial compression on the expression of ET-1,TNF- 伪, IL-1 尾 and IL-6 in serum of pigs with brain death. Methods: twelve male Landrace pigs were randomly divided into two groups. Control group: intracranial pressure probe and water sac catheter were placed in the brain, but no intracranial pressure was carried out. Brain death group: intracranial pressure probe and water sac catheter were placed in the brain. According to the relationship between the intracranial pressure and the mean arterial pressure, the brain death model was established by adjusting the pressure intensity. The brain death state was maintained by respiration and circulation for 12 hours, and the serum ET-1, was measured. TNF- 伪, IL-1 尾 and IL-6 levels. Results: the levels of ET-1 in brain death group increased gradually from 0 h after brain death, and the levels of TNF- 伪, IL-1 尾 and IL-6 increased from 6 h after brain death. The levels of these factors were significantly higher than those in control group (P0.05). Conclusion: the increase of TNF- 伪, IL-1 尾, IL-6 and other inflammatory factors in brain death is relatively slow, while the level of ET-1 changes earlier and the sensitivity is high, which may be related to its role as a proinflammatory mediator.
【作者单位】：天津医科大学一中心临床学院;天津市第一中心医院;
【基金】：国家863计划项目(2012AA021001) 卫生部2013年度国家临床重点专科建设资助项目(卫办医政函[2013]544) 天津市卫生局科技基金(13KG102) 天津市自然科技基金(15JCYBJC27000)
【分类号】：R741

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