TRPV4在小鼠脑血管内皮细胞中的表达及对血管张力调节作用研究

发布时间：2019-05-17 09:42

【摘要】：目的比较瞬时受体电位(TRP)通道家族中香草素受体亚家族(TRPV)和经典瞬时感受器电位亚家族(TRPC)在小鼠脑、心和肝微血管内皮细胞表达差异,寻找脑微血管中具有特异性高表达亚型,阐明其对脑动脉血管舒张的调节作用。方法挖掘GEO数据库中芯片表达谱数据,分析各个通道亚型差异表达情况,再采用免疫组化检测TRPV4、TRPP2和TRPC1在脑基底动脉中表达情况,血管张力实验检测TRPV4通道在调节小鼠脑基底动脉舒张中的效应。结果通过分析表达谱数据显示,在小鼠脑、心和肝三种微血管内皮中,TRPV4通道在脑微血管内皮细胞显著高表达,而TRPC3通道显著低表达。TRPV4、TRPP2和TRPC1在脑基底动脉内皮和平滑肌层也显著表达。TRPV4通道激动剂GSK1016790A能浓度依赖地舒张苯肾上腺素引起的脑基底动脉收缩,而且其舒张效应可以被TRPV4通道阻断剂HC067047所阻断。结论与心和肝血管内皮细胞相比,TRPV4通道在小鼠脑血管内皮细胞中显著高表达,可能在调节脑血管内皮功能中具有重要意义。
[Abstract]:Objective to compare the expression of vanillin receptor subfamily (TRPV) and classical transient receptor potential subfamily (TRPC) in mouse brain, heart and liver microvascular endothelial cells. To explore the specific high expression subtypes of cerebral microvessels and elucidate its regulatory effect on cerebral artery vasodilation. Methods the microarray expression profile data in GEO database were excavated, the differential expression of each channel subgroup was analyzed, and then the expression of TRPV4,TRPP2 and TRPC1 in cerebral basilar artery was detected by immunohistochemistry. The effect of TRPV4 channel on the relaxation of basilar artery in mice was detected by vascular tension test. Results the expression profile data showed that TRPV4 channel was significantly overexpressed in cerebral microvascular endothelial cells, while TRPC3 channel was significantly lower in mouse brain, heart and liver microvascular endothelial cells. TRPV4, TRPV4, TRPP2 and TRPC1 were also significantly expressed in the endothelial and smooth muscle layers of basilar artery. TRPV4 channel agonist GSK1016790A could relax the contraction of basilar artery induced by phenylephrine in a concentration-dependent manner, and its relaxation effect could be blocked by TRPV4 channel blocker HC067047. Conclusion compared with cardiac and hepatic vascular endothelial cells, TRPV4 channel is significantly overexpressed in mouse cerebral vascular endothelial cells, which may play an important role in the regulation of cerebral vascular endothelial function.
【作者单位】：安徽医科大学六安市人民医院神经内科;安徽医科大学基础医学院生理学教研室;
【基金】：国家自然科学基金(编号:81371284)
【分类号】：R743

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