氢溴酸西酞普兰预处理对大鼠局灶脑缺血再灌注损伤的影响

发布时间：2019-05-20 10:15

【摘要】：目的观察氢溴酸西酞普兰预处理对大鼠局灶脑缺血再灌注损伤所致脑梗死体积、海马CA1区细胞凋亡及超微结构的影响。方法将90只SD大鼠分为假手术组、缺血再灌注组(模型组)、氢溴酸西酞普兰预处理高、中、低剂量组(给药剂量分别为20、10、5mg/kg,每天灌胃给药1次,持续7d),末次灌胃2h后采用线栓法制作大脑中动脉缺血2h再灌注模型,再灌注24h后,红四唑氮(TTC)染色法检测脑梗死体积,透射电镜观察损伤侧海马CA1区细胞超微结构,TUNEL法检测损伤侧海马CA1区细胞凋亡并采用激光共聚焦显微镜进行观察和数据测定。结果经氢溴酸西酞普兰预处理各剂量组与模型组相比,大鼠脑梗死体积减小,差异有统计学意义(P0.05);海马CA1区凋亡细胞表达明显减弱,差异有统计学意义(P0.05);透射电镜观察可见氢溴酸西酞普兰预处理各剂量组大鼠损伤侧脑海马CA1区细胞超微结构损伤较模型组明显减轻;以上改变高剂量预处理组效果更为明显。结论氢溴酸西酞普兰预处理可减少脑缺血再灌注损伤所致细胞凋亡和梗死面积,具有脑保护作用。
[Abstract]:Objective to observe the effects of citalopram hydrobromide pretreatment on cerebral infarction volume, apoptosis and ultrastructure in hippocampal CA1 region induced by focal cerebral ischemia-reperfusion injury in rats. Methods 90 SD rats were divided into three groups: sham operation group, ischemia-reperfusion group (model group), high, middle and low dose groups pretreated with citalopram hydrobromide (the dosage was 20 mg 路kg ~ (- 1), 10 mg 路kg ~ (- 1), 5 mg 路kg ~ (- 1), respectively), and the rats were given intragastrically once a day for 7 days. The cerebral infarction volume was measured by red tetrazolium (TTC) staining and the ultrastructure of hippocampal CA1 cells in the injured side was observed by transmission electron microscope after 2 hours of middle cerebral artery ischemia for 2 hours. After 24 hours of reperfusion, the volume of cerebral infarction was measured by red tetrazolium nitrogen staining, and the ultrastructure of hippocampal cells in the injured side was observed by transmission electron microscope. Apoptosis in hippocampal CA1 region of injured side was detected by TUNEL assay and observed and measured by laser confocal microscope. Results compared with the model group, the cerebral infarction volume of each dose group pretreated with citalopram hydrobromide was significantly lower than that of the model group (P 0.05), and the expression of apoptotic cells in hippocampal CA1 region was significantly decreased (P 0.05). Transmission electron microscope observation showed that the ultrastructure damage of hippocampal CA1 cells in each dose group was significantly less than that in the model group, and the effect of the above changes was more obvious in the high dose pretreatment group than that in the model group. Conclusion citalopram hydrobromide pretreatment can reduce apoptosis and infarction area induced by cerebral ischemia-reperfusion injury, and has cerebral protective effect.
【作者单位】：唐山职业技术学院;华北理工大学医学实验研究中心;唐山市协和医院神经内科;
【基金】：河北省科学技术研究与发展项目(132777218) 河北省唐山市科学技术研究与发展项目(12130268b)
【分类号】：R743.3

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