下调LSINCT5对人脑胶质瘤细胞系U251增殖、迁移和放疗敏感性的影响

发布时间：2019-05-24 20:42

【摘要】：目的观察下调LSINCT5对人脑胶质瘤细胞系U251增殖、迁移能力和放疗敏感性的影响。方法采用慢病毒转染法构建稳定干扰LSINCT5的细胞株(U251 sh LSINCT5细胞),得到干扰效率高的sh LSINCT5 1#组、sh LSINCT5 3#组和转染GV248的对照组细胞进行后续实验。采用噻唑蓝比色法(MTS)观察细胞培养0、24、48、72h时增殖能力变化,划痕修复实验观察细胞迁移能力的变化,Western blotting法检测细胞中细胞黏附分子(E-cadherin)、紧密连接蛋白-1(Zo-1)、细胞骨架蛋白(Vimentin)表达,MTS法观察X射线仪下分别照射0、2、4、8 Gy后细胞增殖能力。结果 sh LSINCT5 1#组、sh LSINCT5 3#组培养48、72 h时细胞增殖能力均较对照组低(P均0.05)。sh LSINCT5 1#组、sh LSINCT5 3#组细胞的修复距离均较对照组短,P均0.05。与对照组相比,sh LSINCT51#组、sh LSINCT5 3#组E-cadherin、Zo-1表达高,Vimentin表达低(P均0.05)。sh LSINCT5 1#组、sh LSINCT5 3#组在2、4 Gy剂量照射时细胞增殖能力均较对照组低(P均0.05)。结论下调人胶质瘤细胞系U251中LSINCT5的表达,能抑制细胞增殖、转移,并增强细胞放疗敏感性。
[Abstract]:Objective to observe the effect of down-regulation of LSINCT5 on proliferation, migration and radiotherapy sensitivity of human glioma cell line U251. Methods the cell lines stably interfering with LSINCT5 (U251 sh LSINCT5 cells) were constructed by lentivirus transfer method, and the cells in sh LSINCT5 1 # group, sh LSINCT5 3 # group and GV248 transgenic control group with high interference efficiency were obtained. (MTS) assay was used to observe the proliferation of cells cultured for 0, 24, 48 and 72 h, and the cell migration ability was observed by scratch repair assay. Cell adhesion molecule (E-cadherin) was detected by, Western blotting assay. The expression of tight junction protein-1 (Zo-1) and cytoskeleton protein (Vimentin) were observed by MTS assay. The proliferation ability of the cells was observed by MTS assay after irradiation with 0, 4 and 8 Gy, respectively. Results the proliferation ability of cells in sh LSINCT5 1 # group and sh LSINCT5 3 # group was lower than that in control group at 72 h (P < 0.05). Sh LSINCT5 1 #). The repair distance of cells in sh LSINCT5 3 # group was shorter than that in control group (P 0.05). Compared with the control group, the expression of Zoo1 and Vimentin in sh LSINCT51# group and sh LSINCT5 3 # group were higher and lower than those in control group (all P 0.05). Sh LSINCT51# group, P < 0.05). The proliferation ability of sh LSINCT5 3 # group was lower than that of the control group at the dose of 2 and 4 Gy (P < 0.05), and the cell proliferation ability of the control group was lower than that of the control group (P < 0.05). Conclusion the down-regulation of LSINCT5 expression in human glioma cell line U251 can inhibit the proliferation and metastasis of human glioma cell line U251 and enhance the radiosensitivity of human glioma cell line U251.
【作者单位】：海南医学院第二附属医院;海南医学院;
【分类号】：R739.41

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