红曲素对大鼠脑出血后脑保护作用机制的探讨

发布时间：2019-06-15 03:56

【摘要】：目的探讨红曲素(monascin)在脑出血后脑保护作用的机制及最佳剂量。方法 2016年3月—2016年10月于山西医科大学神经电生理实验室选取雄性SD大鼠120只,随机分为假手术组、模型组、低剂量红曲素组、中剂量红曲素组、高剂量红曲素组各24只。采用Ⅳ型胶原酶定位注射制备大鼠脑出血模型,于术后1、3、7、14 d行Garcia神经功能缺损评分,行脑水含量测定、铁染色及PPAR-γ、Nrf2、SRA测定。结果与假手术组相比,模型组、低剂量组、中剂量组及高剂量组神经功能缺损评分在1、3、7、14 d显著降低(F=181.705、494.229、226.576、24.726,P0.01),且在1、3 d评分最低,脑水含量在1、3 d显著增高(F=21.781、70.036,P0.05),铁沉积颗粒量在3、7、14 d显著增多(F=21.426、118.122、107.773,P0.05),且于7~14 d达高峰,PPAR-γ免疫阳性细胞数于1、3、7 d显著增多(F=3.394、28.622、14.12,P0.05),在3~7 d达高峰,Nrf2免疫阳性细胞数于1、3、7、14 d增多(F=33.076、23.17、47.779、12.182,P0.05),SRA免疫阳性细胞数于1、3、7、14 d显著增多(F=22.283、511.092、51.811、31.060,P0.05)。与模型组相比,高剂量组Garcia评分在3~7 d时升高(P0.05),脑含水量及铁染色颗粒量降低(P0.05),高剂量组PPAR-γ、Nff2及SPA免疫阳性细胞数于1、3及7 d明显升高(P0.05),3~7 d达高峰,14 d降至模型组水平。结论高剂量红曲素可增加脑出血后PPAR-γ、Nrf2及SPA阳性细胞数,减轻脑出血后脑水肿及脑铁含量,改善脑出血后神经功能缺损。
[Abstract]:Objective to investigate the protective mechanism and optimal dose of Monascus (monascin) in the brain after intracerebral hemorrhage (ICH). Methods from March 2016 to October 2016, 120 male SD rats were randomly divided into sham operation group, model group, low dose Monascus group, middle dose Monascus group and high dose Monascus group. The rat model of intracerebral hemorrhage was established by localized injection of type IV collagenase. Garcia neurological deficit score, brain water content, iron staining, PPAR- 纬 and Nrf2,SRA were measured at 1, 3, 7 and 14 days after operation. Results compared with the false operation group, the neurological deficit scores of the model group, the low dose group, the middle dose group and the high dose group were significantly decreased at the 1st, 7th and 14th day (F 鈮，

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