miRNA-210在新生大鼠缺氧缺血性脑水肿中的作用

发布时间：2019-06-15 20:57

【摘要】：目的探讨mi RNA-210在新生大鼠缺氧缺血性脑水肿中的作用。方法将80只新生大鼠随机分为对照组、生理盐水组、mi RNA-210表达抑制组及mi RNA-210过表达组,每组20只;各组大鼠再随机分为假手术组及缺氧缺血(HI)组,每组10只。HI组新生大鼠结扎左侧颈总动脉后,置于8%O2和92%N2的混合气体缺氧舱中持续2 h;假手术组仅游离左侧颈总动脉,但不予结扎和缺氧处理。HI或假手术术后,分别向生理盐水组、mi RNA-210表达抑制组和mi RNA-210过表达组大鼠颅内注射生理盐水(2.5 mg/kg),mi RNA-210incubitor(2.5 mg/kg)和mi RNA-210 minic(2.5 mg/kg),对照组大鼠不予任何处理。3 d后断头,取左侧脑组织,采用荧光定量PCR方法检测mi RNA-210的表达;采用干湿质量法检测脑组织含水量;苏木精-伊红染色观察脑组织形态学改变。结果各组HI新生大鼠脑组织中mi RNA-210的表达较相应假手术组均显著下调,脑组织含水量显著上调(P0.05)。与生理盐水HI组比较,mi RNA-210表达抑制HI组中mi RNA-210的表达显著下调,脑组织含水量显著上调(P0.05);mi RNA-210过表达HI组中mi RNA-210的表达显著上调,脑组织含水量显著下调(P0.05)。苏木精-伊红染色结果提示mi RNA-210表达抑制HI组水肿改变明显;mi RNA-210过表达HI组水肿改变明显改善。结论新生大鼠脑HI后mi RNA-210表达下调,可能参与了新生大鼠缺氧缺血性脑水肿的发生发展。
[Abstract]:Objective to investigate the role of mi RNA-210 in anoxic ischemic brain edema in neonatal rats. Methods 80 neonatal rats were randomly divided into control group, saline group, mi RNA-210 expression inhibition group and mi RNA-210 overexpression group with 20 rats in each group, and the rats in each group were randomly divided into pseudo-operation group (n = 10) and hypoxia-ischemic (HI) group (n = 10). In HI group, the left common carotid artery was ligated and placed in the mixed gas hypoxia chamber of 8%O2 and 92%N2 for 2 hours. The left common carotid artery was only free in the pseudo-operation group, but not treated with ligation and hypoxia. Hi or pseudo-operation, the rats in the saline group, the mi RNA-210 expression inhibition group and the mi RNA-210 overexpression group were injected with normal saline (2.5 mg/kg), mi RNA-210incubitor (2.5 mg/kg) and mi RNA-210 minic (2.5 mg/kg) respectively), while the rats in the control group were not treated with any treatment. 3 days later, the left brain tissue was decapitated and the left brain tissue was taken. The expression of mi RNA-210 was detected by fluorescence quantitative PCR. The water content of brain tissue was measured by dry and wet mass method, and the morphological changes of brain tissue were observed by hematoxylin and eosin staining. Results the expression of mi RNA-210 in brain tissue of HI neonatal rats in each group was significantly lower than that in the corresponding pseudo-operation group, and the water content of brain tissue was significantly up-regulated (P 0.05). Compared with saline HI group, the expression of mi RNA-210 in HI group was significantly down-regulated, and the water content of brain tissue was significantly up-regulated (P 0.05). The expression of mi RNA-210 in HI group was significantly up-regulated and the water content of brain tissue was significantly down-regulated (P 0.05). The results of hematoxylin and eosin staining suggested that the edema changes in HI group were significantly decreased by mi RNA-210 expression, and the edema changes in mi RNA-210 overexpression HI group were significantly improved. Conclusion the down-regulation of mi RNA-210 expression in neonatal rat brain after HI may be involved in the occurrence and development of hypoxic-ischemic brain edema in neonatal rats.
【作者单位】： 南京医科大学附属南京儿童医院新生儿科;
【基金】：南京市科技发展计划项目(201001090) 南京市卫生局医学科技发展项目(YKK10046) “十二五”南京市卫生青年人才工程
【分类号】：R742
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本文编号：2500510