The mRNA Expression Study for Target Genes of miR-246and miR

发布时间：2021-08-17 19:34

　　It has been known that many C. elegans genes can function similarly to mammalian genes. And its genome is surprisingly similar to that of humans （40%homologous）. So C. elegans becomes an attractive organism in the study of human diseases. Epilepsy is one of the most common neurological disorders, which is characterized by paroxysmal electrical disturbances of the brain that are manifested as seizures. Most epilepsy researchers have chosen to limit their studies to mammalian organisms, such as mo... 

【文章来源】：东北师范大学吉林省 211工程院校 教育部直属院校

【文章页数】：41 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
CHAPTER Ⅰ:INTRODUCTION
    1.1 CAENORHABDITIS ELEGANS
    1.2 MICRORNAS
        1.2.1 Identification of microRNAs
        1.2.2 Expression of miRNAs
        1.2.3 miRNA biogenesis and mechanism
        1.2.4 Biological function of miRNAs in C.elegans
            1.2.4.1 Developmental timing in worms
            1.2.4.2 Neuronal left/right asymmetry in worms
    1.3 EPILEPSY STUDY IN C.ELEGANS
        1.3.1 miRNAs in neurons function
        1.3.2 The target gene study of miRNAs
    OBJECTIVES AND JUSTIFICATION OF THE STUDY
CHAPTER Ⅱ:MATERIALS AND METHODS
    2.1 WORMS
    2.2 THE CULTIVATION OF WORMS
        2.2.1 Reagents
        2.2.2 Method
    2.3 THE SYNCHRONIZATION OF WORMS
        2.3.1 Reagents
        2.3.2 Method
    2.4 EXTRACTION TOTAL RNA IN WORMS
        2.4.1 Material and reagent
        2.4.2 RNA extraction procedure
        2.4.3 Detemination the purity and concentration of RNA
    2.5 REVERSE TRANSCRIPTION(RT)
    2.6 PCR
CHAPTERⅢ:RESULTS
    3.1 SELECTED SEVERAL TARGET GENES OF MIR-246 AND MIR-357-358 PREDICTED BY MICROCOSM
    3.2 THE MRNA LEVEL OF GPA-13 WAS INCREASED IN MIR-246 MUTANT WORMS
    3.3 KQT-3,KVS-1,KLC-2 AND JNK-1 MAY BE THE TARGET GENES OF MIR-357-358
CHAPTERⅣ:DISCUSSION
ACKNOWLEDGEMENT
DEDICATION
REFERENCE



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