组织工程半月板:种子细胞和理化因素

发布时间：2017-12-28 00:19

本文关键词：组织工程半月板:种子细胞和理化因素　出处：《中国组织工程研究》2016年51期 　论文类型：期刊论文

【摘要】：背景:由于半月板切除后引起的种种不良后果,人们寄希望生物材料学和生物工程学领域能利用组织工程学的方法修复无血供区半月板损伤,种子细胞的选择以及合适的理化刺激是构建组织工程半月板成功与否的关键。目的:就目前构建组织工程半月板种子细胞及理化因素的研究现状进行综述。方法:由第一作者检索中国知网及Medline数据库中有关构建组织工程半月板种子细胞及理化因素的相关文章,中文检索词为"半月板,组织工程,种子细胞,理化因素",英文检索词为"meniscus,tissue engineering,seed cells,physical and chemical factors"。排除研究目的与课题无关的文章以及重复研究,最后纳入49篇文献作进一步分析。结果与结论:构建的组织工程半月板需要建立再现性,必须让种子细胞在理化刺激并复合支架后继续维持其表型和合成能力。目前,已经确定有很多细胞因子可用于促进软骨细胞培养的增殖和分化,但是这些细胞因子的作用机制和调节因素尚不完全清楚,它们在半月板组织工程重建的应用尚处在探索阶段,仍需进一步研究。实现膝关节组织工程半月板,理化刺激可能是目前最需要改进的地方。剪切力通常被对软骨细胞表型认为是有害的,振荡流体已证明,平行板流室的半月板细胞会产生钙信号和糖胺聚糖。使用剪切和其他因素生成的纤维软骨,在未来可能使组织工程半月板受益。精心设计的实验研究:前瞻性、随机性、对照临床试验与长期定量测量结果,是这个领域的研究评价的关键之处。
[Abstract]:Background: due to various adverse consequences caused by meniscectomy, people hope biological materials science and biological engineering field can use the method of tissue engineering repair without blood donor meniscus injury, seed cell selection and physicochemical proper construction of tissue engineering meniscus stimulation is the key to success. Objective: To review the present status of the research on the construction of seed cells and physicochemical factors of the tissue engineering meniscus. Methods: the first author retrieved relevant articles about construction and physicochemical factors of seed cells in tissue engineering meniscus Chinese HowNet and Medline database, the key words of "Chinese meniscus, tissue engineering, seed cells, physical and chemical factors, English key words were" meniscus, tissue engineering, seed cells, physical and chemical factors". In the end, 49 articles were included for further analysis. Results and conclusion: the tissue-engineered meniscus is needed to establish reproducibility. We must enable seed cells to maintain their phenotype and synthesis ability after physical and chemical stimulation and composite scaffolds. At present, there are many cytokines have been identified can be used to promote chondrocytes proliferation and differentiation, but the mechanism factors of these cytokines and regulation is not fully understood, their application in meniscus tissue engineering is still in the exploratory stage, further research is needed. The physical and chemical stimulation of the knee joint tissue engineering meniscus may be the most important place to be improved at present. Shear forces are generally considered to be harmful to the phenotype of chondrocytes, and oscillatory fluids have shown that meniscus cells in parallel plate cells produce calcium signals and glycosaminoglycan. Fibrous cartilage generated using shear and other factors may benefit the tissue engineering meniscus in the future. Carefully designed experimental studies: prospective, randomized, controlled clinical trials and long-term quantitative measurements are key points in the research evaluation in this field.
【作者单位】：内蒙古医学院第二附属医院关节镜与运动医学外科;内蒙古医学院第二附属医院门诊部;
【基金】：内蒙古自治区自然基金资助项目(2016MS0366),项目名称:脂肪干细胞诱导仿生修复半月板损伤及其相关实验研究~~
【分类号】：R318.08;R687.4
【正文快照】： 0引言Introduction 目前治疗无血供区半月板撕裂的主要方式是半月板部分或全部切除术,手术后往往会引起关节软骨退变而导致骨性关节炎。由于半月板切除后引起的种种不良后果,人们寄希望生物材料学和生物工程学领域能利用组织工程学的方法修复无血供区半月板损伤。到目前为止,

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