磷酸钙陶瓷骨支架的激光烧结机理及工艺研究

发布时间：2017-12-31 04:24

本文关键词：磷酸钙陶瓷骨支架的激光烧结机理及工艺研究　出处：《中南大学》2013年硕士论文　论文类型：学位论文

【摘要】：摘要：制备具有优良的机械性能和生物学性能的骨支架已经成为生物制造研究领域的热点。磷酸钙生物陶瓷因与自然骨有相似的无机成分和晶体结构,具有良好的生物相容性,能与骨形成化学键性结合,可诱导骨组织再生,但由于脆性大、韧性低以及降解速率不可控等缺点,限制了它在骨组织工程中的应用。本文通过添加增强相来改善磷酸钙生物陶瓷骨支架的性能,重点研究了增强相对支架机械性能和生物学性能的影响规律与作用机理。主要研究工作与创新性成果如下： 1、利用ANSYS有限元分析软件中的APDL参数化语言实现了激光高斯热源的移动加载,分析了SLS过程中三维瞬态温度场及其随热载荷移动速度的变化规律,优化获得理想的激光扫描速度为50-250mm/min。 2、利用SLS技术制备了三维多孔β-TCP骨支架,研究了不同激光扫描速度下所制备支架的平均晶粒大小和机械性能的关联规律。研究了晶粒尺寸对细胞在支架上粘附性能的影响,MG63成骨样细胞和人骨髓间充质干细胞能够在支架上粘附和生长,且支架的晶粒尺寸越小越有利于细胞的粘附和增殖,采用体外模拟体液(SBF)浸泡实验研究了支架的生物活性,支架显示出良好的生物活性。 3、添加ZnO来提高支架的性能,研究了ZnO的含量对支架的微观结构、物相组成和机械性能的影响。发现ZnO的添加能够提高支架的机械性能,且最佳的添加比例为2.5%。 4、研究了ZnO含量对骨支架细胞粘附性能和降解性能的影响,MG63细胞能够在支架上很好的粘附。支架的降解速率随着ZnO含量的增加不断减慢,且提高了支架的生物活性。
[Abstract]:Abstract: the preparation of bone scaffolds with excellent mechanical and biological properties has become a hotspot in the field of biological manufacturing. Calcium phosphate bioceramics have similar inorganic composition and crystal structure to natural bone. It has good biocompatibility, can bind to bone to form chemical bond, and can induce bone regeneration, but because of its high brittleness, low toughness and uncontrollable degradation rate. The application of calcium phosphate bioceramics in bone tissue engineering is limited. In this paper, the performance of calcium phosphate bioceramic bone scaffold is improved by adding reinforcement phase. The effects of reinforcement on the mechanical and biological properties of scaffolds are studied. The main research and innovative results are as follows: 1. The moving loading of laser Gao Si heat source is realized by using APDL parameterized language in ANSYS finite element analysis software. The variation of 3D transient temperature field and its moving velocity with thermal load in SLS process is analyzed. The optimal laser scanning speed is 50 ~ 250 mm / min. 2. Three-dimensional porous 尾 -TCP bone scaffolds were fabricated by SLS technique. The relationship between the average grain size and the mechanical properties of the scaffolds prepared at different laser scanning speeds was studied, and the effect of grain size on the adhesion of cells on the scaffolds was studied. MG63 osteoblast-like cells and human bone marrow mesenchymal stem cells can adhere to and grow on the scaffold, and the smaller the grain size of the scaffold, the more conducive to cell adhesion and proliferation. The bioactivity of the scaffold was studied by SBF immersion in vitro, and the scaffold showed good bioactivity. The effects of ZnO content on the microstructure, phase composition and mechanical properties of the scaffolds were studied. It was found that the addition of ZnO could improve the mechanical properties of the scaffolds. The optimum addition ratio is 2.5. 4. The effect of ZnO content on the adhesion and degradation of bone scaffold cells was studied. MG63 cells could adhere to the scaffold very well, and the degradation rate of scaffold decreased with the increase of ZnO content. The bioactivity of the scaffold was improved.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

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