纳米细菌纤维素膜的表征与生物相容性研究

发布时间：2018-01-01 03:10

本文关键词：纳米细菌纤维素膜的表征与生物相容性研究　出处：《高等学校化学学报》2009年08期 　论文类型：期刊论文

【摘要】：利用木醋杆菌静态培养法制备的由纳米纤维组成的细菌纤维素膜具有超细的三维网络结构和适当的孔隙率.利用光镜、扫描电镜和原子力显微镜对其进行结构表征发现,细菌纤维素膜具有极为精细的纳米网络结构,冻干膜的孔径约为0.6~2.8μm;纤维素带宽度约为50~80 nm.采用湿重与浮重结合法测定烘干膜和冻干膜的孔隙率分别约为70%和90%.由于细菌纤维素含有大量的羟基,故烘干膜表现出极好的透湿性.将细菌纤维素膜分别与成纤维细胞和软骨细胞进行复合培养,并将成纤维细胞和细菌纤维素膜的复合物进行裸鼠皮下移植实验.结果显示,移植的复合物很好地融入了裸鼠正常皮肤,成纤维细胞和软骨细胞在细菌纤维素表面形成连续的细胞层,绿色荧光蛋白表达正常.以上结果表明,细菌纤维素膜非常适合细胞贴附和增殖,表现出较好的生物相容性,有望成为新型组织工程支架材料.
[Abstract]:The use of a.xylinum static culture bacterial cellulose membrane composed of nanofibers prepared by the three-dimensional network structure with ultra-fine and appropriate porosity. Using light microscopy, scanning electron microscopy and atomic force microscopy was used to characterize the discovery of bacterial cellulose membrane has very fine nano network structure, pore size of freeze-dried film about 0.6~2.8 m; cellulose with a width of about 50~80 nm. by wet weight and determination of drying and freeze dry film floating film weight method of porosity were approximately 70% and 90%. due to bacterial cellulose contains many hydroxyl groups, the drying film exhibits excellent permeability. The bacterial cellulose membrane with fibroblasts and chondrocytes cells were co cultured, and compound fibroblasts and bacterial cellulose membranes were transplanted subcutaneously in nude mice. The experimental results show that the compound transplantation well into the normal skin of nude mice, Fibroblast cells and cartilage cells formed a continuous layer on the surface of bacterial cellulose, the expression of green fluorescent protein. The above results showed that the bacterial cellulose membrane is very suitable for cell adhesion and proliferation, exhibit good biocompatibility, is expected to become a new scaffold material for tissue engineering.

【作者单位】：吉林大学药学院;中国科学院长春应用化学研究所高分子物理与化学国家重点实验室;天津科技大学材料科学与化学工程学院;天津教育部工业微生物重点实验室;
【基金】：吉林省科技厅项目(批准号:20050401-2,200705386) 天津科技大学人才引进基金(批准号:20070443)资助
【分类号】：R318.08
【正文快照】： 目前,各种各样的天然高分子材料(如胶原、壳多糖、明胶等)被广泛用于生物医学的研究,例如组织工程支架、药物释放系统和生物传感器等.由细菌生物合成的纤维素(Bacterial cellulose,简称BC)是另一种天然高分子材料,因其具有许多优异的化学、物理、机械和生物学性能,并在制造先

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