掺钾纳米羟基磷灰石对牙本质小管及其表面硬度、粗糙度影响的研究

发布时间：2018-01-18 12:41

本文关键词：掺钾纳米羟基磷灰石对牙本质小管及其表面硬度、粗糙度影响的研究　出处：《福建医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：研究目的进行固定冠桥修复时，，为了满足修复体所需要的足够空间，临床上往往需要磨除大量牙体组织，导致牙本质敏感。牙本质敏感的发病机制目前有三种假说，其中流体动力学理论被广泛接受，该理论认为，作用于牙本质外部的刺激引起了牙本质小管内容物向内或向外的异常流动，这种液体流动刺激到牙髓，引起牙髓神经的兴奋，产生痛觉。目前，临床上所使用的脱敏剂基本上都是基于这个理论研制而成。纳米羟基磷灰石及其复合物在口腔治疗及保健领域的应用越来越多的受到国内外学者的关注，有研究表明纳米羟基磷灰石能有效封闭牙本质小管，本实验用掺钾纳米羟基磷灰石处理牙本质表面，扫描电镜下观察这种新型材料封闭离体牙本质小管的情况，测量其对牙本质表面硬度及粗糙度是否产生影响，以期为新型牙脱敏材料的研制提供理论依据。实验方法收集因正畸拔除、完整无龋坏的上颌第一前磨牙，制备牙本质薄片，分别用Gluma脱敏剂、纳米羟基磷灰石、掺钾纳米羟基磷灰石处理牙本质表面，扫描电镜下观察其封闭牙本质小管的情况，用显微硬度计和粗糙度测量仪分别测量牙本质表面硬度值以及粗糙度值的改变情况，将所得到的结果用SPSS13.0软件进行统计学分析。实验结果 1、扫描电镜下，三种不同处理材料均能有效封闭牙本质小管口，掺钾纳米羟基磷灰石能够深入到牙本质小管内部约16μm、纳米羟基磷灰石能够深入到牙本质小管内部约12μm。 2、经掺钾纳米羟基磷灰石处理过的牙本质表面，硬度平均值约为58.2733HV，硬度值高于未做任何处理的牙本质样本。 3、经掺钾纳米羟基磷灰石处理过的牙本质表面，扫描电镜下可见牙本质小管口几乎全部被封闭，牙本质表面平整度不一，有斑块状突起，粗糙度平均值为3.7851μm，高于未做任何处理的牙本质样本。结论 1、掺钾纳米羟基磷灰石能有效封闭牙本质小管，并深入到牙本质小管内部约16μm。 2、掺钾纳米羟基磷灰石提高牙本质表面维氏硬度值。 3、掺钾纳米羟基磷灰石使牙本质表面粗糙度Ra值增大。
[Abstract]:Research purpose In order to meet the need of enough space for the restoration of fixed crown and bridge, there are three hypotheses about the pathogenesis of dentin sensitivity, which usually needs to remove a large number of dentin tissues in clinic. Among them, the theory of hydrodynamics is widely accepted, which holds that the stimulation acting on the external dentin causes abnormal flow of the contents of the dentine tubules, which stimulates the dental pulp. Causes the pulp nerve to excite, produces the pain sensation. At present. The desensitizer used in clinic is based on this theory. The application of nano-hydroxyapatite and its complex in the field of oral treatment and health care has attracted more and more attention of scholars at home and abroad. Some studies have shown that nano-hydroxyapatite can effectively seal the dentine tubules. In this experiment, potassium doped nano-hydroxyapatite was used to treat the dentin surface. Scanning electron microscope was used to observe the effect of this new material on the isolated dentine tubules. In order to provide a theoretical basis for the development of new desensitization materials, the influence on the hardness and roughness of dentin surface was measured. Experimental method The maxillary first premolars without caries were extracted by orthodontics and the dentin slices were prepared. The dentin surface was treated with Gluma desensitizer nano-hydroxyapatite and potassium doped nano-hydroxyapatite respectively. The microhardness and roughness of the dentine surface were measured by microhardness meter and roughness tester respectively. The results were analyzed by SPSS13.0 software. Experimental results 1. Under scanning electron microscope, the dentine tubules could be effectively sealed by three different materials, and potassium doped nano-hydroxyapatite could penetrate into the dentin tubules about 16 渭 m. Nano hydroxyapatite can penetrate into the dentin tubules about 12 渭 m. 2, the average hardness of the dentine treated with potassium doped nano-hydroxyapatite is about 58.2733 HVV, which is higher than that of the untreated dentin sample. 3. On the surface of dentin treated with potassium doped nano-hydroxyapatite, almost all dentine tubules were closed under scanning electron microscope. The mean roughness was 3.7851 渭 m, which was higher than that of untreated dentin samples. Conclusion 1. Potassium doped nano-hydroxyapatite can effectively seal the dentine tubules and penetrate into the dentin tubules about 16 渭 m. 2. Potassium-doped nano hydroxyapatite increased Vickers hardness of dentin surface. 3. K-doped nano hydroxyapatite increases the roughness Ra value of dentin surface.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R783.1

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