周期性拉伸应力与人永生化角质形成细胞的黏附和铺展

发布时间：2018-01-23 04:26

本文关键词： 生物力学应力物理细胞粘附细胞增殖干细胞培养周期性拉伸应力人永生化角质形成细胞 HaCaT 铺展纽蛋白增殖黏附国家自然科学基金　出处：《中国组织工程研究》2015年01期 　论文类型：期刊论文

【摘要】：背景:皮肤组织所处的力学环境以及上皮细胞的铺展状态与伤口愈合和瘢痕形成过程关系密切。目的:分析胞外力学刺激对细胞铺展的作用,并检测细胞的增殖状态进一步分析铺展形态对细胞增殖等生理活动的影响。方法:通过FX-4000柔性基底加载系统对人永生化角质形成细胞(HaCaT)进行周期性拉伸应力正弦波加载,加载程式0.2 Hz,10%拉伸幅度。在0,24,48 h对细胞的铺展形态进行对比,利用流式细胞仪检测分析细胞的增殖,并利用免疫荧光染色方法对比分析纽蛋白在细胞内的分布。结果与结论:HaCaT细胞在加载24 h后分裂期细胞较多,铺展形态无明显变化;加载48 h后HaCaT细胞形态发生较为明显的变化,分裂期细胞较静态对照组略有减少;拉伸应力作用下纽蛋白的分布由细胞核周围的膜区域向细胞边缘集中。结果表明:适度的力学加载能够在保持细胞铺展和黏附状态下,促进细胞增殖;周期性持续拉伸应力的作用时间是HaCaT细胞铺展形态和与基底黏附位点的重要影响因素。
[Abstract]:Background: the mechanical environment of skin tissue and the spreading state of epithelial cells are closely related to wound healing and scar formation. Objective: to analyze the effect of extracellular mechanical stimulation on cell spreading. The effects of spreading morphology on cell proliferation and other physiological activities were analyzed. Methods: human immortalized keratinocytes were treated by FX-4000 flexible substrate loading system. HaCaT is subjected to sine wave loading of cyclic tensile stress. The extension range of 0.2 Hzn was 10%. The proliferation of the cells was analyzed by flow cytometry after 48 hours. The distribution of neutropin in the cells was analyzed by immunofluorescence staining. Results and conclusion: after 24 hours of loading, there were more cells in the mitotic phase, but no obvious changes in the spreading morphology of the cells. After 48 h of loading, the morphologic changes of HaCaT cells were more obvious, and the number of cells in the mitotic phase was slightly lower than that in the static control group. Under tensile stress, the distribution of Neuron was concentrated from the membrane area around the nucleus to the edge of the cell. The results showed that moderate mechanical loading could promote cell proliferation while keeping the state of cell spreading and adhesion. The duration of cyclic continuous tensile stress is an important factor affecting the spreading morphology of HaCaT cells and the adhesion sites to the substrate.
【作者单位】：太原理工大学;
【基金】：国家自然科学基金支持(10672114) 山西省自然科学基金支持(2007011011)~~
【分类号】：R318.08
【正文快照】： 0引言Introduction应用基底变形技术将机械应力作用于培养的细胞,研究力学刺激对细胞增殖、凋亡、分化、细胞力学特性及基因和蛋白表达等的影响,是目前较常用的生物力学研究方法。近年来机械刺激的生物学效应及它们的作用机制已在多种细胞和组织中开展。体内的表皮组织处于预

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