异种骨与成骨细胞体外生物相容性研究

发布时间：2018-01-27 23:53

本文关键词： 异种骨成骨细胞生物相容性　出处：《中国矫形外科杂志》2014年02期 　论文类型：期刊论文

【摘要】：[目的]评价异种骨与成骨细胞体外生物相容性,为其在临床应用提供实验依据。[方法]将SD大鼠来源的成骨细胞分别在普通培养基和含异种骨浸提液的培养基中培养,于1、3、5、7 d用四甲基偶氮唑盐(methyl thiazolyltetrazolium,MTT)法测定吸光度值,以评价细胞的增殖情况。采用Transwell试验,在倒置相差显微镜下观察细胞的迁移情况,计算穿膜成骨细胞数量,检测成骨细胞的迁移能力。应用流式细胞仪检测细胞周期变化,以评价异种骨浸提液对成骨细胞周期的影响。[结果]成骨细胞在普通培养基和含异种骨浸提液的培养基中采用MTT检测增殖结果无显著性意义(P0.05)。在含异种骨浸提液的培养基中,成骨细胞在Transwell中穿膜细胞数为(13.40±2.51)个/视野,对照组为(8.00±1.87)个/视野,两组差异有显著性意义(P0.05),实验组细胞迁移数量多于对照组。流式细胞仪检测显示,异种骨浸提液对细胞周期无影响。[结论]异种骨与成骨细胞有良好的生物相容性,为其临床应用提供了试验依据。
[Abstract]:[Objective] to evaluate the biocompatibility of bone xenograft and osteoblast in vitro and to provide experimental evidence for its clinical application. [Methods the osteoblasts derived from SD rats were cultured in normal culture medium and medium containing heterologous bone extract, respectively. The absorbance was determined by methyl thiazolyl tetrazolium MTT on 7 days. In order to evaluate cell proliferation, Transwell test was used to observe the migration of cells under inverted phase contrast microscope and to calculate the number of transmembrane osteoblasts. The migration ability of osteoblasts and the changes of cell cycle were detected by flow cytometry to evaluate the effect of heterogenous bone extract on the cell cycle of osteoblasts. [Results: the proliferation of osteoblasts was detected by MTT in the ordinary culture medium and the medium containing the heterologous bone extract. There was no significant difference in the proliferation of osteoblasts in the culture medium containing the heterologous bone extract. The number of perforating cells in Transwell was 13.40 卤2.51 / visual field, while that in control group was 8.00 卤1.87 / visual field. The difference between the two groups was significant (P 0.05). The number of cell migration in the experimental group was more than that in the control group. Flow cytometry showed that the heterogenous bone extract had no effect on the cell cycle. [Conclusion: xenogenic bone has good biocompatibility with osteoblasts, which provides experimental basis for its clinical application.
【作者单位】：南方医科大学研究生院;广州军区广州总医院骨科医院;山东省医疗器械产品质量检验中心山东省医疗器械生物学评价重点实验室;
【基金】：再生型医用置入器械国家工程实验室项目(编号:2012NELRMD007)
【分类号】：R318.08
【正文快照】： 由创伤、肿瘤或代谢疾病等造成的骨缺损发生率很高,如何实现骨缺损的重建,一直以来是骨科医生面临的一个极具挑战的临床难题[1-2]。自体骨是骨移植的“金标准”[3],但取材有限、增加创伤,此外相关研究还发现供区二次手术会增加导致畸形和手术死亡率的风险[4-5]。异种骨作为一

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