弱激光和LED光对离体兔红细胞影响的实验研究

发布时间：2018-02-01 02:05

本文关键词： 弱激光 LED 红细胞溶血光敏化　出处：《南京理工大学》2012年硕士论文　论文类型：学位论文

【摘要】：弱激光和LED光应用于临床照射治疗,已取得多方面疗效,受到很大重视,有重要的应用前景。然而其治疗机理尚未有明确认识,相关基础研究有所不足,一些疑团有待解决。本文通过实验研究了低强度623nm、636nm LED光和405nm半导体激光照射兔离体红细胞所产生的促溶血效应。实验中,采集了实验兔的新鲜血液,抗凝处理,生理盐水洗涤,配置成所需浓度的红细胞混悬液,分别设置LED光和激光照射组和对照组,检测和对比红细胞溶血率的数值。结果表明,在一定条件下623nm和636 nm LED照射组的溶血率显著高于对照组,且随照射功率的增大而升高；405nm激光照射组溶血率与对照组无显著差异。本文还对比研究了405nm、532nm、632.8nm、660nm激光和532nm、623nm、636nmLED光照射后的红细胞溶血率,探索了不同波长对红细胞溶血效应的影响机理。对比结果表明：(1)红光(623nm、632.8nm、636nm、660nm)对兔红细胞有显著的促溶血作用,而405nm紫光和532nm绿光没有明显的促溶血效应；(2)红光(623nm、632.8nm、636nm、660nm)中,以632.8nm激光的促溶血作用最强,相同的照射功率下,血样的溶血率最高；660nm激光在较低功率时,促溶血的程度较低,但当照射功率达30-40mW后,随照射功率增大,促溶血的程度也明显变大；(3)红光照射产生的促溶血效应都具有“延迟发生”的特点；(4)在红光波段,不同波长光的促溶血作用强度与原卟啉受激后在该波长附近的荧光强度呈正相关关系。通过分析上述实验结果和对比研究结果的生物学意义及其影响因素,提出了作者的观点,认为红细胞内源性卟啉经弱激光或LED光照射可引发光敏化反应,进而导致系列生物学效应。在弱激光和LED光照射治疗过程中,这些效应可起重要作用。红细胞对紫光、绿光的吸收和对红光的吸收机理不同,导致了紫光、绿光与红光对红细胞溶血影响的差异。本文研究结果可为进一步研究光与血液相互作用的机理,弱激光和LED光照射疗法的治疗机理,以及弱激光和LED光的生物效应及机理,提供基础研究依据和理论指导。
[Abstract]:The application of weak laser and LED light in clinical irradiation treatment has obtained many curative effects, has been paid great attention to, and has important application prospects. However, its therapeutic mechanism has not been clearly understood, and the related basic research is insufficient. Some doubts remain to be solved. In this paper, the hemolytic effect of low intensity 636nm LED light and 405nm semiconductor laser irradiation on rabbit erythrocytes in vitro was studied. The fresh blood, anticoagulant treatment, normal saline washing, red blood cell suspension of the required concentration were collected, and the LED light and laser irradiation group and the control group were set up respectively. The results showed that the hemolysis rate of 623nm and 636nm LED irradiation group was significantly higher than that of the control group under certain conditions, and increased with the increase of irradiation power. There was no significant difference in hemolysis rate between 405nm laser irradiation group and control group. The erythrocyte hemolysis rate of 405nmO5nmO532nmO632.8nm 660nm laser and 532nmC623nmP636nmLED was also studied in this paper. The effects of different wavelengths on erythrocyte hemolysis were investigated. 660nm) had significant hemolytic effect on rabbit red blood cells, but 405nm purple light and 532nm green light had no obvious hemolytic effect. (2) in 623 nm (623 nm), 636 nm (660 nm), 632.8 nm laser had the strongest hemolytic effect under the same irradiation power. The hemolysis rate of blood sample was the highest. The degree of hemolysis promoted by 660nm laser at low power was lower, but when the irradiation power reached 30-40MW, the degree of hemolysis was obviously increased with the increase of irradiation power. (3) the hemolytic effect of red light irradiation has the characteristic of "delayed occurrence"; (4) in the red light band, the intensity of hemolysis of different wavelengths of light is positively correlated with the fluorescence intensity of protoporphyrin in the vicinity of this wavelength. Based on the analysis of the biological significance of the above experimental results and the comparative study results and their influencing factors, this paper puts forward the author's view that red blood cell endogenous porphyrin irradiation with weak laser or LED light can induce Guang Min reaction. These effects may play an important role in the treatment of weak laser and LED light irradiation. The absorption mechanism of red blood cells to purple light, green light and red light is different, leading to purple light. Difference between green light and red light on erythrocyte hemolysis. The results can be used to further study the mechanism of the interaction between light and blood, the therapeutic mechanism of low laser and LED light irradiation, and the biological effect and mechanism of weak laser and LED light. To provide basic research basis and theoretical guidance.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.51

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