葛根素对纯镁微弧氧化植酸涂层表面生长的成骨细胞的影响

发布时间：2018-02-04 04:58

本文关键词： 葛根素纯镁植酸成骨细胞　出处：《中国体视学与图像分析》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨不同浓度的葛根素溶液对纯镁经超声微弧氧化植酸处理后表面生长的成骨细胞的影响,确定最适宜成骨细胞生长的浓度。方法取四组不同浓度组:A组0 mol/L葛根素溶液,B组1×10~(-8)mol/L葛根素溶液,C组1×10~(-6)mol/L葛根素溶液,D组1×10~(-4)mol/L葛根素溶液,通过CCK-8法检测不同时间点纯镁试件表面生长的成骨细胞的黏附、增殖状况,激光共聚焦显微镜下观察细胞的早期骨架形态。结果纯镁经超声微弧氧化后植酸处理达到封孔效果,成骨细胞附着于试件生长后加入相应浓度的葛根素溶液,四组实验CCK-8黏附、增殖数据显示C组B组A组,A组与D组之间未见统计学差异,激光共聚焦显微镜下显示C组的细胞形态效果最好。结论 1×10~(-6)mol/L葛根素溶液为促进纯镁UMAO植酸涂层表面的成骨细胞生长的最适宜浓度。
[Abstract]:Objective to investigate the effects of different concentrations of puerarin solution on the growth of osteoblasts on the surface of pure magnesium treated by ultrasonic micro-arc oxidation of phytic acid. Methods the optimal concentration of osteoblasts for osteoblast growth was determined. Methods four groups of different concentration groups were taken from 0 mol/L puerarin solution and 1 脳 10 ~ (-8) mol / L puerarin solution in group B and 1 脳 10 ~ (-1) mol 路L ~ (-1) 路L 路L puerarin solution. Group C 1 脳 10 ~ (-6) mol / L puerarin solution and D group 1 脳 10 ~ (-4) mol 路L ~ (-1) puerarin solution. The adhesion and proliferation of osteoblasts grown on the surface of pure magnesium at different time points were detected by CCK-8 method. The morphology of early cytoskeleton was observed under laser confocal microscope. Results the microarc oxidation of pure magnesium with phytic acid could seal the pores and the osteoblasts were attached to the specimen and then added puerarin solution of the corresponding concentration. Four groups of experimental CCK-8 adhesion, proliferation data showed that C group B group A group A group A and D group no statistical difference between the two groups. The effect of laser confocal microscope on cell morphology in group C is the best. Conclusion 1 脳 10 ~ (-1) -6). Mol/L puerarin solution was the most suitable concentration for promoting the growth of osteoblasts on the surface of pure magnesium UMAO phytic acid coating.
【作者单位】：佳木斯大学黑龙江省高校生物医学材料重点实验室;
【基金】：佳木斯大学研究生科技创新项目(YM2016_076) 国家自然科学基金(No.31370979) 黑龙江省高校创新团队建设计划项目(2012TD010)
【分类号】：R318.08
【正文快照】： 0引言目前临床应用的骨内固定金属材料有不锈钢、钛合金和钴合金,这些材料在短期植入手术中往往需要二次手术取出,增加了患者的痛苦和细菌感染的风险,因此亟待于发展新型短期植入骨材料。纯镁具有良好的比强度、比刚度及生物相容性,其弹性模量与骨的弹性模量接近,使对影响骨再

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