SPIO标记大鼠骨髓间充质干细胞:生物学活性及体外MRI成像效应评价

发布时间：2018-02-04 05:09

本文关键词： 超顺磁性氧化铁粒子磁共振成像骨髓间充质干细胞　出处：《生物医学工程学杂志》2014年02期 　论文类型：期刊论文

【摘要】：为研究不同浓度(25、50、100μg/mL)超顺磁性氧化铁粒子(SPIO)标记对大鼠骨髓间充质干细胞(rMSCs)生物学活性、体外MRI成像效应的影响,采用多聚赖氨酸(PLL)包被的SPIO(PLL-SPIO)标记原代分离培养的rMSCs,24h后分别进行阳性标记率、MRI成像效应、细胞增殖凋亡以及干细胞细胞分化能力的综合评价。PLLSPIO标记阳性率、铁含量测定结果显示:铁标记率可达75%~100%。透射电镜观察,可见100μg/mL组rMSCs细胞质内浓聚细小铁颗粒的囊泡样包涵体。采用T1WI、T2WI和T2*WI序列MRI成像扫描显示:25μg/mL组即可引起信号的明显降低。与未标记的对照组细胞相比,25μg/mL和50μg/mL组细胞增殖活力、凋亡、干细胞分化能力检测结果差异皆无统计学意义(P0.05),而100μg/mL组的差异均有统计学意义(P0.05)。结论:25、50μg/mL SPIO可有效标记rMSCs,100μg/mL的SPIO对rMSCs增殖活性和分化能力有抑制作用,这为SPIO进一步的体内及临床MRI分子影像应用提供了基础。
[Abstract]:In order to study the biological activity of superparamagnetic iron oxide (SPIOO) labeled rat bone marrow mesenchymal stem cells (BMSCs) with different concentrations of 2550g / mL superparamagnetic iron oxide (SPIOO). The effect of MRI imaging in vitro was labeled with poly-lysine-coated SPIO-PLL-SPIO-labeled primary isolated and cultured rMSCs. After 24 hours, positive labeling rate and MRI imaging effect, cell proliferation and apoptosis and differentiation ability of stem cells were evaluated. The results of determination of iron content showed that the rate of iron labeling could reach 75% and 100%. The vesicular inclusion bodies in the cytoplasm of rMSCs were found in 100 渭 g / mL group. T1WI was used. T2WI and T2WI MRI images showed that the signal intensity was significantly decreased in the 25 渭 g / mL group compared with the unlabeled control group. In 25 渭 g / mL and 50 渭 g / mL groups, there was no significant difference in cell proliferation activity, apoptosis and stem cell differentiation ability (P 0.05). The difference of 100 渭 g / mL SPIO was statistically significant (P 0.05). Conclusion 1: 2550 渭 g / mL SPIO can effectively label rMSCs. 100 渭 g / mL of SPIO inhibited the proliferation and differentiation of rMSCs, which provided the basis for the further application of SPIO in vivo and clinical MRI molecular imaging.
【作者单位】：新疆医科大学基础医学院;四川大学华西基础与法医学院;四川大学实验动物中心;
【基金】：国家自然科学基金项目资助课题(11262020) 新疆维吾尔自治区自然科学基金资助项目(201233146-9) 四川省应用基础计划项目(2013JY0021)
【分类号】：R318.5;R445.2
【正文快照】： 引言近年来,随着科学技术的发展,分子生物学与现代医学影像技术相互结合,形成一门新兴的边缘学科——分子影像学(molecular imaging)[1]。目前,分子影像学主要包括核素成像、光学成像和磁共振成像(magnetic resonance imaging,MRI)三大类。在这三类成像技术中,MRI以其三维采集

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