新型蜂巢样聚己内酯-硅酸钙复合晶体材料修复颅骨极限缺损

发布时间：2018-03-02 20:22

本文选题：颅骨　切入点：骨再生　出处：《中国组织工程研究》2017年18期 　论文类型：期刊论文

【摘要】：背景:聚己内酯作为高分子材料的生物相容性较差,需将其与其他天然生物材料复合增加生物相容性,促进机体组织再生。目的:观察新型蜂巢样聚己内酯-硅酸钙复合晶体材料在SD大鼠颅骨缺损中的成骨修复作用。方法:取18只SD大鼠,制作颅骨极限缺损模型,随机分3组干预,空白组不植入任何材料,对照组植入普通聚己内酯-硅酸钙复合晶体材料,实验组植入新型蜂巢样聚己内酯-硅酸钙复合晶体材料。植入6周后,进行骨缺损处X射线扫描、Micro-CT三维重建及组织学分析。结果与结论:(1)X射线扫描:3组大鼠颅骨缺损变小,骨折线模糊,边缘骨密度增高,实验组、对照组新生骨面积百分比明显大于空白组,且实验组新生骨面积百分比大于对照组;(2)Micro-CT三维重建:空白组新生骨主要分布在缺损两侧,对照组、实验组新生骨分布于整个颅骨骨缺损处;与空白组比较,实验组、对照组均表现出更强的再生骨能力(P0.05),且以实验组成骨能力最强(P0.05);(3)组织学分析:3组均可见骨缺损处有不同程度新生骨长入,实验组新生骨组织及新生血管密度明显高于对照组、空白组(P0.05);(4)结果表明:新型蜂巢样聚己内酯-硅酸钙复合晶体材料在颅骨缺损生物环境中具有明显的促成骨作用。
[Abstract]:Background: the biocompatibility of polycaprolactone as a polymer is poor. It is necessary to combine it with other natural biomaterials to increase the biocompatibility. Objective: to observe the effect of a new hive like polycaprolactone / calcium silicate compound crystal material in repairing the skull defect of SD rats. Methods: eighteen SD rats were selected to make the model of skull limit defect. Three groups were randomly divided into three groups: the blank group did not implant any material, the control group implanted the common polycaprolactone calcium silicate compound crystal material, the experimental group implanted the new honeycomb polycaprolactone calcium silicate compound crystal material. After 6 weeks of implantation, the experimental group implanted the new honeycomb polycaprolactone calcium silicate composite crystal material. Three-dimensional reconstruction and histological analysis of micro-CT in bone defect were performed. Results and conclusion the skull defect was smaller, the fracture line was blurred, the bone density was increased in the experimental group, and the bone density was increased in the experimental group. The percentage of new bone area in the control group was significantly larger than that in the blank group, and the percentage of the new bone area in the experimental group was larger than that in the control group. The newborn bone in the experimental group was distributed in the whole skull defect, and compared with the blank group, the experimental group, In the control group, the bone regeneration ability was higher than that in the control group (P 0.05), and the osteogenic ability of the experimental group was the strongest (P 0.05). Histological analysis showed that there were different degrees of new bone growth in the bone defect in the three groups. The density of the new bone tissue and the neovascularization vessel in the experimental group was significantly higher than that in the control group. The results showed that the new honeycomb polycaprolactone-calcium silicate composite crystal material had a significant role in promoting bone in the biological environment of skull defect.
【作者单位】：南方医科大学南方医院关节与骨病外科;
【基金】：广东省自然科学基金(2014A030313275,s2013010014253) 南方医科大学“国家杰出青年培养计划”项目~~
【分类号】：R318.08;R651.1

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