包芯骨组织工程仿生支架的制备、表征和体外细胞相容性研究

发布时间：2018-03-03 18:56

本文选题：骨组织工程　切入点：快速成形　出处：《新疆医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：探讨提高包芯骨组织工程仿生支架的亲水性及体外细胞相容性的方法，为体内实验提供研究依据。方法：采用复合喷射低温沉积快速成形工艺（MSLDM）制备快速成形支架，以明胶改性的PLGA／β-TCP支架（包芯骨支架）作为实验组，未经明胶修饰的PLGA／β-TCP支架（原始支架）作为对照组，检测两组支架的孔隙率、力学性能、支架表面元素分析、亲水性、降解率、蛋白吸附能力；将MSCs接种于包芯骨支架和原始支架，分别测定检测支架材料的体外细胞相容性（包括细胞黏附率、细胞增殖率，扫描电镜观察细胞黏附形态）。结果：两组支架大体观察均形成三级孔隙结构；包芯骨支架孔隙率为85.71%±2.05%，原始支架孔隙率为82.81%±0.9%，，（P0.05）；能谱分析包芯支架表面的氮元素重量百分比为7.31%、原子百分比为8.39%；包芯骨支架的压缩模量和抗压强度优于原始支架（P＜0.05）；包芯骨支架的亲水率在1h和168h均高于原始支架（P＜0.05）；两组支架的体外降解率没有明显差异（P＞0.05）；包芯骨支架蛋白吸附能力明显高于原始支架（P＜0.05）；细胞在接种12h和24h时，原始支架的细胞粘附率明显少于包芯骨支架（P＜0.05）；包芯骨支架在培养6d、8d、10d时的细胞增殖率明显高于原始支架（P＜0.05）；SEM计数MSCs粘附于支架表面，包芯骨支架6d、8d、10d的细胞数量均多于原始支架（P＜0.05）。结论：经明胶改性的PLGA／β-TCP支架亲水性得到改进、体外降解性不受影响，且具有良好的细胞相容性，可用于骨组织工程支架修复骨缺损的体内研究，具有潜在临床应用价值。
[Abstract]:Objective: to explore a method to improve the hydrophilicity and in vitro cytocompatibility of scaffolds for bone tissue engineering. Methods: rapid prototyping scaffolds were prepared by composite spray low temperature deposition rapid prototyping (MSLDM). The PLGA/ 尾 -TCP scaffold modified by gelatin was used as experimental group and PLGA/ 尾 -TCP scaffold without gelatin modified as control group. The porosity, mechanical properties, surface element analysis, hydrophilicity and degradation rate of the two groups were measured. MSCs was inoculated into the core-bone scaffold and the original scaffold, and the cell compatibility (including cell adhesion rate, cell proliferation rate) of the scaffold was measured. Results: the morphology of cell adhesion was observed by scanning electron microscope. The porosity of the core-bone scaffold was 85.71% 卤2.05, the porosity of the original scaffold was 82.81% 卤0.9and that of the original scaffold was 82.81% 卤0.90.The weight percentage of nitrogen on the surface of the core scaffold was 7.31 and the atomic percentage was 8.390.The compression modulus and compressive strength of the core-bone scaffold were better than those of the original scaffold (P < 0.05). The hydrophilic rate of core bone scaffold was higher at 1 h and 168 h than that of original scaffold (P < 0.05), there was no significant difference in in vitro degradation rate between the two groups (P > 0.05), the protein adsorption ability of core bone scaffold was significantly higher than that of the original scaffold (P < 0.05), and the cells were inoculated at 12 h and 24 h after inoculation. The cell adhesion rate of the original scaffold was significantly lower than that of the core-bone scaffold (P < 0.05), and the cell proliferation rate of the core-bone scaffold was significantly higher than that of the original scaffold (P < 0.05) when cultured for 6 days or 8 days or 10 days, and the number of MSCs adhered to the surface of the scaffold was significantly higher than that of the original scaffold (P < 0.05). The number of cells in the scaffold was more than that in the original scaffold (P < 0.05). Conclusion: the hydrophilicity of PLGA/ 尾 -TCP scaffold modified by gelatin has been improved, the biodegradability of the scaffold is not affected in vitro, and it has good cytocompatibility. It can be used to repair bone defect with bone tissue engineering scaffold in vivo, and has potential clinical application value.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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