HSNGLPL短肽修饰的聚氨酯的体内反应性分析

发布时间：2018-03-04 00:35

本文选题：聚氨酯　切入点：HSNGLPL短肽　出处：《中国临床解剖学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的研究短肽HSNGLPL修饰的聚氨酯在体内与体外的生物相容性与生物活性。方法将单纯的HSNGLPL短肽溶液、三种不同浓度HSNGLPL短肽修饰的聚氨酯(P-PU)及聚氨酯(BDOPU)注射或种植在小鼠体内或在体外与C2C12细胞共培养,通过HE检测体内局部炎症渗出、免疫荧光检测体内单核/巨噬细胞渗出凋亡和肌纤维的再生以及通过细胞增殖毒性反应检测体外共培养结果。结果在体外,BDO-PU、L-P-PU和M-P-PU是合格的生物材料,具有优良的生物相容性;在体内,BDO-PU只在材料28d诱发持久的单核/巨噬细胞的渗出和肌纤维的坏死,而P-PU在肌肉内诱发的炎症、坏死反应比BDO-PU更加复杂、持久,持续时间甚至超过56d。对于细胞凋亡而言,P-PU材料周围凋亡的巨噬细胞数却明显的低于BDO-PU组。结论 HSNGLPL修饰的聚氨酯在体内与体外具有生物活性及优良的生物相容性。
[Abstract]:Objective to study the biocompatibility and bioactivity of polyurethane modified with short peptide HSNGLPL in vitro and in vivo. Three different concentrations of HSNGLPL short peptide modified polyurethane (Pu) and polyurethane (Pu) were injected or planted in mice or co-cultured with C2C12 cells in vitro. The exudation of local inflammation was detected by HE. Apoptosis and muscle fiber regeneration were detected by immunofluorescence. Results BDO-PUL-P-PU and M-P-PU were qualified biomaterials with excellent biocompatibility in vitro. In vivo, BDO-PU induced persistent mononuclear / macrophage exudation and myofibrillar necrosis only at 28 d, while P-PU induced inflammation in the muscle, which was more complicated and lasting than BDO-PU. The number of apoptotic macrophages around P-PU material was significantly lower than that in BDO-PU group. Conclusion HSNGLPL modified polyurethane has bioactivity and excellent biocompatibility in vitro and in vivo.
【作者单位】：南方医科大学解剖学教研室广东省组织构建与检测重点实验室;华南理工大学材料科学与工程学院;
【基金】：国家自然科学基金(81371924,81572102) 广东省自然科学基金(2014A030313276)
【分类号】：R318.08

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