丝素降解生成的低分子多肽对细胞生长性能的影响

发布时间：2018-03-06 09:52

本文选题：低分子丝素蛋白多肽　切入点：酶解　出处：《丝绸》2017年04期 　论文类型：期刊论文

【摘要】：分析丝素降解生成的低分子多肽(low-molecular-weight silk fibroin peptides,LMW-SF)对细胞生长的影响。LMW-SF通过中性蛋白酶酶解丝素蛋白(silk fibroin,SF)获得,分别采用过滤除菌和高压蒸汽灭菌处理后,在0.1~5.0 mg/m L体积浓度条件下,检测LMW-SF对人正常肝细胞(L02)、人肝癌细胞(HepG2)的生长影响,计算相对增殖率(relative growth rate,RGR)。结果显示:当LMW-SF体积浓度较低(0.1 mg/m L和0.5 mg/m L)时,LMW-SF对细胞生长影响较小;当其体积浓度较高(2.5 mg/m L和5.0 mg/m L)时,RGR值最低至8.3%,呈强抑制细胞生长作用;高压蒸汽灭菌处理后,RGR最高达227.4%,呈强促细胞生长作用。研究结果表明:高压蒸汽处理可以使LMW-SF变性失活,并实现强促细胞生长作用,可成为蛋白类物质应用于组织工程支架时的必需工艺环节。
[Abstract]:Low molecular weight polypeptide analysis of silk fibroin degraded (low-molecular-weight silk fibroin peptides, LMW-SF) effects on cell growth of.LMW-SF by neutral protease hydrolysis of silk fibroin (silk fibroin, SF), were used to filter sterilization and steam sterilization treatment, in the 0.1~5.0 mg/m L concentration under the condition of LMW-SF on normal human detection liver cells (L02), human hepatocellular carcinoma cell (HepG2) growth effect, calculate the relative growth rate (relative growth, rate, RGR). The results showed that when the LMW-SF with low concentration (0.1 mg/m L and 0.5 mg/m L), LMW-SF had little effect on the growth of cells; when the concentration is higher (2.5 mg/m L mg/m and 5 L), the lowest RGR value to 8.3%, showed strong growth inhibitory effects; high pressure steam sterilization treatment, RGR up to 227.4%, a strong role in promoting cell growth. The results show that the high pressure steam treatment can make LMW-SF instability Living, and achieving strong cell growth, can be a necessary process for the application of protein substances to tissue engineering scaffolds.

【作者单位】：重庆理工大学药学与生物工程学院;
【基金】：重庆市科委(csct2014jcyja10018) 重庆市教委(KJ1400916)
【分类号】：R318.08

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