应用纤维蛋白胶复合细胞膜片构建人工软骨

发布时间：2018-03-06 12:23

本文选题：纤维蛋白胶　切入点：细胞膜片　出处：《中山大学学报(医学科学版)》2017年01期 　论文类型：期刊论文

【摘要】：【目的】利用细胞膜片具备的良好的流动性,以及纤维蛋白胶的可塑性为其塑形,构建具备空间立体结构的软骨块。【方法】采用兔耳软骨细胞在培养瓶中进行体培养形成细胞膜片,刮取后剪碎,另取离散软骨细胞,用纤维蛋白胶两种组分混悬细胞膜片和离散软骨细胞,滴加到48孔板中,在体外构建出圆片状的结构,经过体外培养后进一步植入裸鼠体内,培养8周后取材。采用大体观察及组织学、免疫组织化学等方法观察分析体内外培养的结构。【结果】获得成熟的圆片状组织工程软骨结构,组织学表现为成熟的软骨组织,免疫组化结果提示软骨细胞外基质内II型胶原蛋白高表达。但结构内软骨组织分布不均匀,岛状软骨组织之间的间隙内为未降解的纤维蛋白胶成分。在其中1个圆片结构中,发现局部骨化现象。【结论】纤维蛋白胶是一种良好的载体,用于复合软骨细胞膜片及软骨细胞,可成功构建出具备空间立体结构人工软骨结构。
[Abstract]:[objective] to make use of the good fluidity of cell membrane and the plasticity of fibrin glue. The chondrocytes with three-dimensional structure were constructed. [methods] chondrocytes of rabbit ear were cultured in culture flask to form cell membrane, then scraped and shredded, and then separated chondrocytes. Two components of fibrin glue were used to mix cell membrane slices and discrete chondrocytes into 48 hole plates to construct round flake structures in vitro. After cultured in vitro, they were further implanted into nude mice. After 8 weeks of culture, the materials were collected and analyzed by gross observation, histology, immunohistochemistry and other methods. [results] mature round tissue engineering cartilage structure was obtained, which showed mature cartilage tissue. The immunohistochemical results showed that type II collagen was highly expressed in the extracellular matrix of chondrocytes, but the distribution of cartilage in the structure was not uniform, and the gap between the island cartilage tissues was composed of undegraded fibrin glue. [conclusion] Fibrin glue is a good carrier for composite chondrocyte membrane and chondrocyte, and can be successfully constructed with three-dimensional structure of artificial cartilage.
【作者单位】：解放军第306医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金青年科学基金项目(81401609)
【分类号】：R318.17

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