制备组织工程脱细胞真皮修复皮肤溃疡创面的愈合机制

发布时间：2018-03-09 10:06

本文选题：烧伤　切入点：皮肤　出处：《中国组织工程研究》2016年25期 　论文类型：期刊论文

【摘要】：背景:近年来,人尸体脱细胞真皮在真皮缺损、软组织充填中得到应用,但是该修复材料价格昂贵,来源相对有限。目的:分析组织工程脱细胞真皮修复皮肤溃疡创面的效果。方法:随机对照方法将S D大鼠分为自体未脱细胞网状层真皮组和脱细胞真皮组。采用低渗溶液、2.5 g/L胰蛋白酶、体积分数0.5%Triton X-100和PBS方法制备组织工程脱细胞真皮,建立大鼠皮肤溃疡创面模型,自体未脱细胞网状层真皮组移植自体断层皮片进行覆盖,脱细胞真皮组采用新型颗粒状组织工程脱细胞真皮进行修复,比较两组修复效果及对皮肤溃疡创面愈合的机制。结果和结论:(1)获得的组织工程脱细胞真皮为白色状,常温下大体观察呈颗粒状;(2)苏木精-伊红染色显示组织工程脱细胞真皮内部呈现出"树枝状"分布,并且胶原纤维束的间隙相对较大;(3)脱细胞真皮组植皮后2,3,4,8周创面移植皮肤成活率,显著高于自体未脱细胞网状层真皮组(P0.05);(4)脱细胞真皮组大鼠植皮后3,4,8周创面收缩率显著高于自体未脱细胞网状层真皮组(P0.05);(5)脱细胞真皮组创周正常皮肤胶原纤维束平均直径及间隙率显著高于自体未脱细胞网状层真皮组(P0.05)。(6)结果提示:组织工程脱细胞真皮制备方法相对简单,制备条件温和,且制备的组织工程脱细胞真皮形态规则,修复皮肤溃疡创面效果理想,能降低真皮内D NA残留量,能显著改善体内某些生物力学特性,促进创面愈合。
[Abstract]:Background: in recent years, human cadaveric acellular dermis has been used in dermis defect and soft tissue filling, but the material is expensive. Objective: to analyze the effect of tissue engineering acellular dermis on the repair of skin ulcer wound. Methods: SD rats were randomly divided into two groups: autologous unacellular reticular layer dermis group and acellular dermis group. With a hypotonic solution of 2.5 g / L trypsin, Tissue engineering acellular dermis was prepared by Triton X-100 and PBS method. The rat model of skin ulcer was established. Acellular dermis group was repaired with new granular tissue engineering acellular dermis. The effect of the two groups and the mechanism of wound healing of skin ulcer were compared. Results and conclusion the tissue engineered acellular dermis was white. At room temperature, the results of hematoxylin and eosin staining showed that there was a "dendritic" distribution in the acellular dermis of tissue engineering. In acellular dermis group, the survival rate of skin grafts was 8 weeks after skin grafting. The rate of wound contraction in acellular dermis group was significantly higher than that in unacellular reticular layer group (P0.05). The contraction rate of wound in acellular dermis group was significantly higher than that in non-acellular reticular layer group at 8 weeks after skin grafting. The average diameter and interspace rate were significantly higher than those in the unacellular reticular layer group (P0.05. 6). The results showed that the preparation method of tissue engineering acellular dermis was relatively simple. The condition of preparation is mild, and the prepared tissue engineering acellular dermis is regular, the effect of repairing skin ulcer wound is ideal, the content of DNA in dermis can be reduced, some biomechanical properties in vivo can be improved and wound healing can be promoted.
【作者单位】：昆明理工大学附属医院(云南省第一人民医院、昆华医院);
【基金】：云南省教育厅科学研究院基金项目(20144C004Y)~~
【分类号】：R318.08;R641

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