三维打印制备不同大孔形态含锶介孔生物玻璃支架的体外细胞实验

发布时间：2018-03-10 14:33

本文选题：生物相容性材料　切入点：锶　出处：《中国组织工程研究》2017年18期 　论文类型：期刊论文

【摘要】：背景:三维打印支架的大孔孔径形态对生物组织工程支架理化性能及生物性能有很大的影响。目的:利用三维打印技术制备不同大孔结构的含锶介孔生物玻璃支架,探讨其对MC3T3-E1细胞增殖和成骨分化的影响,寻找最佳的大孔形态。方法:利用三维打印技术制备含锶介孔生物玻璃/聚己内酯复合支架,其中纵横交错的各纤维状经纬线之间的夹角设置为45°,60°和90°,根据其角度命名为45°,60°,90°含锶介孔生物玻璃支架,检测MC3T3-E1细胞在复合支架上的增殖能力和碱性磷酸酶活性。结果与结论:(1)CCK-8方法检测结果表明,3组支架材料都能够支持细胞增殖,第1天和第4天45°含锶介孔生物玻璃支架上的细胞数量略高于60°和90°含锶介孔生物玻璃支架,但差异无显著性意义(P0.05);第7天45°含锶介孔生物玻璃支架上的细胞数量显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意义(P0.05);(2)在第14天,45°含锶介孔生物玻璃支架的细胞碱性磷酸酶活性显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意意义(P0.05);在第21天,3组支架的碱性磷酸酶活性差异无显著性意义(P0.05);(3)结果表明,45°含锶介孔生物玻璃支架相较于60°和90°含锶介孔生物玻璃支架,更能促进MC3T3-E1细胞的增殖和成骨分化。
[Abstract]:Background: the macroporous morphology of 3D printing scaffolds has great influence on the physical and chemical properties and biological properties of biomedical scaffolds. Objective: to prepare different macroporous mesoporous bioglass scaffolds with different macroporous structures by using 3D printing technology. In order to find the best macroporous morphology, the mesoporous glass / polycaprolactone composite scaffold containing strontium was prepared by three dimensional printing technique. Among them, the angles between the longitude and theodolite lines are set to 45 掳掳60 掳and 90 掳. According to their angles, they are named as 45 掳~ 60 掳~ 90 掳strontium containing mesoporous bioglass scaffolds, The proliferation and alkaline phosphatase activity of MC3T3-E1 cells on the composite scaffold were measured. Results and conclusions the results of CCK-8 assay showed that all three groups of scaffolds could support cell proliferation. On day 1 and day 4, the number of cells on 45 掳strontium containing mesoporous glass scaffolds was slightly higher than that of 60 掳and 90 掳strontium containing mesoporous glass scaffolds. On the 7th day, the number of cells on 45 掳strontium containing mesoporous glass scaffolds was significantly higher than that on 60 掳and 90 掳strontium containing mesoporous glass scaffolds. The cell alkaline phosphatase activity of 45 掳strontium containing mesoporous glass scaffolds was significantly higher than that of 60 掳and 90 掳strontium containing mesoporous bioglass scaffolds on the 14th day. On the 21st day, there was no significant difference in alkaline phosphatase activity among the three scaffolds. The results showed that 45 掳strontium containing mesoporous glass scaffolds were compared with 60 掳and 90 掳strontium containing mesoporous glass scaffolds. It can promote the proliferation and osteogenic differentiation of MC3T3-E1 cells.
【作者单位】：复旦大学附属浦东医院骨科;
【基金】：上海市科委基础研究领域项目(13JC1407302) 浦东新区卫生系统重点学科群建设资助项目(PWZxq2014-03) 上海市卫生局重点课题(20134039)~~
【分类号】：R318.08

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