血管内皮生长因子纳米微球-细菌纤维素复合支架与多种细胞构建组织工程膀胱

发布时间：2018-03-12 20:10

本文选题：纤维素　切入点：膀胱　出处：《中国组织工程研究》2016年21期 　论文类型：期刊论文

【摘要】：背景:传统膀胱修补的方法存在损伤正常器官功能、术后并发症多等问题,组织工程为膀胱修复提供了新的途径。目的:探讨血管内皮生长因子纳米微球-细菌纤维素支架复合兔舌黏膜上皮细胞及舌骨骼肌细胞构建组织工程膀胱的可行性。方法:分别制备细菌纤维素支架与血管内皮生长因子纳米微球-细菌纤维素支架,将兔舌骨骼肌细胞、舌黏膜上皮细胞依次接种至两种支架上。取6只兔,制备膀胱缺损模型,随机分两组,实验组于缺损处植入复合自体舌骨骼肌细胞、舌黏膜上皮细胞的血管内皮生长因子纳米微球-细菌纤维素支架,对照组于缺损处植入复合自体舌骨骼肌细胞、舌黏膜上皮细胞的细菌纤维素支架。植入后3个月进行膀胱造影、尿动力学及组织学检测。结果与结论:(1)造影结果:实验组可见相对完整的膀胱,对照组部分膀胱可见小面积充盈缺损。(2)尿动力学检测结果:两组的最大膀胱容量和膀胱顺应性均较植入前降低,对照组上述尿动力学参数下降幅度显著高于实验组(P0.05)。(3)组织学检测结果:对照组修复处膀胱组织未能构建出完整的上皮细胞层,其下方仅有少量肌层形成,黏膜下方有少量微血管形成;实验组修复处膀胱组织有完整上皮细胞层形成,可见到黏膜下方有较多肌层形成,黏膜下方有较多量毛细血管形成。(4)结果表明,将血管内皮生长因子纳米微球-细菌纤维素支架与舌骨骼肌细胞、舌黏膜上皮细胞复合可构建组织工程膀胱。
[Abstract]:Background: the traditional method of bladder repair has many problems, such as damage to normal organ function, postoperative complications and so on. Tissue engineering provides a new approach for bladder repair. Objective: to investigate the feasibility of constructing tissue engineered bladder with vascular endothelial growth factor nanospheres and bacterial cellulose scaffolds combined with rabbit tongue mucosal epithelial cells and tongue skeletal muscle cells. Methods: bacterial cellulose scaffolds and vascular endothelial growth factor nanospheres were prepared respectively. Rabbit tongue muscle cells and tongue mucosal epithelial cells were inoculated into two scaffolds successively. Six rabbits were used to establish bladder defect model. The experimental group was randomly divided into two groups. Vascular endothelial growth factor (VEGF) nanospheres and bacterial cellulose scaffolds were implanted into tongue epithelial cells in the control group. Bacterial cellulose scaffolds for tongue epithelial cells. Cystoscopy, urodynamics and histology were performed 3 months after implantation. Results and conclusions: relatively intact bladder was found in the experimental group. The results of urodynamic test showed that the maximal bladder capacity and bladder compliance were lower in both groups than before implantation. The decrease of the above urodynamic parameters in the control group was significantly higher than that in the experimental group (P 0.05). The histological results showed that the bladder tissue in the control group could not construct a complete epithelial cell layer, and only a few myometrium were formed below the bladder tissue in the control group. There was a small amount of microangiogenesis under the mucous membrane, while in the experimental group, there was a complete epithelial cell layer in the bladder tissue, and more myometrium under the mucous membrane, and more capillary formation under the mucous membrane. Tissue engineering bladder could be constructed by combining vascular endothelial growth factor nanospheres-bacterial cellulose scaffold with tongue skeletal muscle cells and tongue mucosal epithelial cells.
【作者单位】：上海交通大学附属第六人民医院泌尿外科;
【分类号】：R318.16

【共引文献】