庆大霉素和O-羧甲基壳聚糖对硫酸钙骨水泥改性研究

发布时间：2018-03-17 01:01

本文选题：硫酸钙骨水泥　切入点：O-羧甲基壳聚糖　出处：《中国修复重建外科杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的通过添加O-羧甲基壳聚糖(oxygen-carboxymethylated chitosan,O-CMC)和庆大霉素对硫酸钙骨水泥(calcium sulfate cement,CSC)进行改性,提高其在骨修复领域应用的可行性。方法通过在CSC液相中添加不同浓度O-CMC(0.1wt%、0.3wt%、0.5wt%、0.7wt%、1.0wt%),制备O-CMC/CSC。通过可注射性、抗压强度、降解率、模拟体液pH值及Ca~(2+)浓度测试、成分与形貌表征观察以及细胞毒性测试,分析O-CMC浓度对CSC在可注射性、抗压强度、降解行为及成骨活性方面的影响。综合以上检测指标,确定最佳性能的O-CMC/CSC,并以此复合不同浓度庆大霉素(0.5wt%、1.5wt%、2.5wt%),探究其抗压强度和抗菌性。结果 O-CMC/CSC可注射时间均超过5 min,且随O-CMC浓度的增加而显著延长(P0.05)。改良后的骨水泥抗压强度介于11~18 MPa之间,0.5wt%O-CMC/CSC抗压强度最高(P0.05)。O-CMC/CSC降解速率随O-CMC浓度增加而呈减小趋势,但组间比较差异无统计学意义(P0.05)。浸泡液pH值变化在7.2~7.4之间,Ca~(2+)浓度稳定在6~8 mmol/L。成分与形貌表征观察显示,相比于CSC,O-CMC/CSC在模拟体液中的诱导矿化能力更强,具有更好生物活性。综合以上检测指标结果,确定0.5wt%O-CMC/CSC性能最佳,加入庆大霉素后其抗压强度均大于5 MPa且具有抗菌性。结论 O-CMC能够有效提高CSC的可注射性、抗压强度和成骨活性,加入庆大霉素后复合骨水泥具有抗菌性。
[Abstract]:Objective to improve the feasibility of calcium sulfate cementation by adding O-carboxymethylated chitosan (O-CMC) and gentamicin to improve the feasibility of its application in the field of bone repair. Methods O-CMC / CSCS was prepared by adding different concentrations of O-CMCU 0.1 wt0. 3wt0. 5wt in CSC liquid phase. Compressive strength, degradation rate, pH value of simulated body fluid and Ca~(2) concentration, composition and morphology characterization and cytotoxicity test were analyzed to analyze the injectability and compressive strength of O-CMC concentration to CSC. Effects of degradation behavior and osteogenic activity. O-CMC / CSCS with different concentrations of gentamicin was used to study the compressive strength and antibacterial properties of O-CMC / CSCS. Results the injection time of O-CMC / CSC was more than 5 min, and the P0.05 was significantly prolonged with the increase of O-CMC concentration. The modified bone cement resistance was improved. The degradation rate of O-CMC / CSC / O-CMC / CSC decreased with the increase of O-CMC concentration, and the compressive strength of O-CMC / CSC was higher than that of O-CMC / CSC with the increase of O-CMC concentration. However, there was no significant difference between the two groups (P 0.05). The pH value of the soaking solution varied from 7.2 to 7.4) and the concentration was stable at 6 ~ 8 mmol 路L ~ (-1) 路L ~ (-1). The composition and morphologic characterization showed that the mineralizing ability of CSCO-CMC / CSC was stronger than that of CSCO-CMC / CSC in simulated body fluid, and the concentration of CSCO-CMC / CSC was higher than CSCO-CMC / CSC. The results showed that O-CMC / CSC / CSC was the best, and the compressive strength of O-CMC / CSC was more than 5 MPa after addition of gentamicin. Conclusion O-CMC can effectively improve the injectability of CSC. The compressive strength and osteogenic activity of composite bone cement with gentamicin were antibacterial.
【作者单位】：天津市胸科医院检验科;天津医科大学医学检验学院;天津大学材料科学与工程学院;
【分类号】：R318.08;R68

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