中空多孔金属假体复合诱导因子对骨髓间充质干细胞生长及成骨分化的影响

发布时间：2018-03-17 02:14

本文选题：骨形态发生蛋白质类　切入点：假体和植入物　出处：《中国组织工程研究》2016年25期 　论文类型：期刊论文

【摘要】：背景:将骨形态发生蛋白与中空多孔钛合金复合,可提升材料与周围骨组织的亲和力。目的:观察骨形态发生蛋白2对中空多孔金属假体支架内骨髓间充质干细胞生长及成骨分化的影响。方法:将第3代SD大鼠骨髓间充质干细胞直接接种于中空多孔金属假体支架上,分别以含0,0.001,0.01,0.06,0.1 g/L骨形态发生蛋白2的DMEM培养基培养,培养0,6,12,24,48 h,采用MTT法检测细胞黏附情况;培养18 d,茜素红染色检测细胞成骨分化能力。将Transwell培养池置于中空多孔金属假体支架上,上室加入5×108 L-1的骨髓间充质干细胞悬液,下室分别加入含0,0.001,0.01,0.06,0.1 g/L骨形态发生蛋白2的DMEM培养基,培养0,6,12,24,48 h后检测细胞迁徙能力。结果与结论:1培养6-48 h,不同质量浓度骨形态发生蛋白2呈时间依赖性促进骨髓间充质干细胞的黏附;2培养18 d,经不同质量浓度骨形态发生蛋白2干预后,骨髓间充质干细胞由梭形改变为多角形,细胞呈多层性、重叠性排列,形成大量钙化结节,茜素红染色呈鲜红色;3培养6-48 h内,骨形态发生蛋白2可促进骨髓间充质干细胞的迁徙,且呈浓度、时间依赖性;4结果表明:骨形态发生蛋白2可增强中空多孔金属假体支架上骨髓间充质干细胞在的黏附能力、成骨分化能力与迁徙能力。
[Abstract]:Background: bone morphogenetic protein (BMP) was combined with hollow porous titanium alloy. To observe the effect of bone morphogenetic protein-2 on the growth and osteogenic differentiation of bone marrow mesenchymal stem cells in hollow porous metal prosthesis scaffolds. Bone marrow mesenchymal stem cells were directly inoculated on hollow porous metal prosthesis scaffolds. The cell adhesion was detected by MTT method in DMEM medium containing 0 0. 001G / L bone morphogenetic protein 2 (0. 001G / L bone morphogenetic protein 2) for 48 h. After cultured for 18 days, alizarin red staining was used to detect the osteogenic differentiation ability of the cells. The Transwell culture cell was placed on a hollow porous metal prosthesis scaffold, and 5 脳 10 8 L ~ (-1) of bone marrow mesenchymal stem cells suspension was added to the upper chamber. DMEM medium containing 0 0. 001G / L bone morphogenetic protein 2 (BMP 2) was added to the lower chamber. Results and conclusion different concentrations of bone morphogenetic protein 2 promoted bone marrow mesenchymal stem cell adhesion to bone marrow mesenchymal stem cells in a time-dependent manner for 18 days, and were cultured with different bone mass concentrations for 6 to 48 hours. After the intervention of morphogenetic protein-2, Bone marrow mesenchymal stem cells changed from fusiform to polygonal, the cells were multilayered, overlapping, and formed a large number of calcified nodules. Alizarin red staining showed bright red for 6-48 hours. Bone morphogenetic protein-2 could promote the migration of bone marrow mesenchymal stem cells in a concentration dependent manner. The results showed that bone morphogenetic protein 2 could enhance the adhesion of bone marrow mesenchymal stem cells on hollow porous metal prosthesis scaffolds. Osteogenic differentiation ability and migration ability.
【作者单位】：青海大学附属医院创伤骨科;
【分类号】：R318.08;R687

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