基于三维打印的钙化层重建生物活性支架制备及其性能研究

发布时间：2018-03-18 01:05

  本文选题：软骨疾病/治疗　切入点：硅　出处：《浙江大学学报(医学版)》2016年02期 　论文类型：期刊论文

【摘要】：目的:初步构建基于软骨钙化层损伤重建的有机—无机复合组织工程支架,探究掺镁硅灰石含量与支架抗压性能之间的关系。方法:利用质量分数分别为1%、3%、5%的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠进行三维打印,经海藻酸钠—氯化钙气雾交联成型,电镜下观察表面孔隙、孔径、无机相分布,万能材料试验机测试抗压性能,并计算支架孔隙率。结果:支架表面平均孔径(212.3±34.2)μm,平均孔隙率(48.3±5.9)%,不同质量分数的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠支架压缩模量差异无统计学意义(P0.05),平均压缩模量(7.2±1.2)MPa,介于软骨和软骨下骨之间。结论:利用三维打印技术成功构建出多孔钙化层仿生重建支架,可为今后研制多层次复合支架治疗骨—软骨损伤奠定基础。
[Abstract]:Objective: to construct an organic-inorganic composite tissue engineering scaffold based on cartilage calcification layer injury reconstruction. The relationship between the content of magnesium-doped wollastonite and the compressive resistance of the scaffold was investigated. Methods: Three-dimensional printing was carried out by using the ultrafine particles of high bioactive calcium magnesium silicate ultrafine particles, type I collagen-sodium hyaluronate, with a mass fraction of 1% or 3%, respectively. The pore size, pore size and distribution of inorganic phase on the surface were observed under electron microscope by crosslinking of sodium alginate and calcium chloride, and the compressive resistance was tested by universal material testing machine. Results: the average pore size of scaffold was 212.3 卤34.2 渭 m and the average porosity was 48.3 卤5.9 渭 m. There was no difference in compression modulus of high bioactive calcium magnesium silicate ultrafine particle composite type I collagen-sodium hyaluronate scaffold with different mass fraction. The mean compression modulus is 7.2 卤1.2 mpa, which is between cartilage and subchondral bone. Conclusion: the biomimetic reconstruction scaffold of porous calcified layer was successfully constructed by three-dimensional printing technique. It can lay a foundation for the treatment of bone-cartilage injury with multi-layer composite scaffold in the future.
【作者单位】： 浙江大学医学院附属第二医院骨科浙江大学骨科研究所;浙江加州国际纳米技术研究院;
【基金】：国家自然科学基金(81472063) 浙江省自然科学基金(LZ14E020001)
【分类号】：R687;R318.08
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