表面沉积掺锶羟基磷灰石的聚乳酸多孔支架的制备及其用于骨修复的研究

发布时间：2018-03-18 02:01

本文选题：掺锶羟基磷灰石　切入点：聚乳酸多孔支架　出处：《河北大学》2017年硕士论文　论文类型：学位论文

【摘要】：骨缺损是临床上的常见病,也是骨科治疗的难题。随着人类对自身健康的关注度随经济发展的提高,生物医用材料产业高速发展。组织工程是应用生命科学和工程学的原理,在体外构建一个有生物活性的三维空间复合物,作为替代物修复或重建器官,从而达到提高生存质量和延长生命活动的目的。目前,传统生物医学材料生物学性能的改进和提高是生物医用材料研究的重点。羟基磷灰石(HA)具有极好的生物相容性和骨传导性,锶(Sr)具有骨诱导性,Sr掺杂的HA(Sr-HA)作为一种新的生物材料可以显著促进MC3T3-E1细胞增殖矿化。考虑到骨的成分是有机无机混合材料,制备聚合物/无机复合支架材料具有十分重要的意义。在以往的聚合物/HA复合支架的制备中,一般都是直接将聚合物与HA粒子混合,这种方法会使大部分生物活性HA包埋在聚合物基体内,并且会造成HA在聚合物基体内分布不均,导致在植入早期HA无法与种子细胞有效接触。本文采用仿生矿化的方法在聚乳酸(PLLA)多孔支架表面沉积掺Sr的HA,制备出仿生矿化的Sr-HA/PLLA多孔支架。这种复合材料表面含有大量的生物无机矿物质,为细胞的黏附和生长提供了更适宜的环境,且更好的模拟天然骨的细胞外基质成分和结构,为骨修复提供一个较好的生物相容环境。通过扫描电镜(SEM)、红外光谱(FT-IR)、XRD、抗压性能、接触角和蛋白吸附实验对其进行表征,结果表明Sr-HA的沉积可以显著改善PLLA材料的疏水性,Sr的掺入明显增加材料的骨诱导性,与传统的HA相比,这种复合材料具有更高的成骨性能和更优良可控的降解性能。体内和体外实验都表明复合材料的生物相容性良好,促进成骨细胞黏附、增殖和分化,并且诱导骨修复。
[Abstract]:Bone defect is not only a common disease in clinic, but also a difficult problem in orthopedic treatment. With the development of economy, the biomedical material industry develops rapidly. Tissue engineering is the application of life science and engineering principle. Construct a bioactive three-dimensional complex in vitro to repair or reconstruct the organ as an alternative, thereby improving the quality of life and prolonging life activities. The improvement and improvement of biological properties of traditional biomedical materials is the focus of biomedical materials research. Hydroxyapatite (HA) has excellent biocompatibility and bone conductivity. As a new biomaterial, Sr) can significantly promote the proliferation and mineralization of MC3T3-E1 cells, considering that the bone is an organic and inorganic mixture. The preparation of polymer / inorganic composite scaffolds is of great significance. In the past polymer / HA composite scaffolds were prepared by mixing polymer directly with HA particles. In this way, most of the bioactive HA is embedded in the polymer group, and the HA is unevenly distributed in the polymer group. In this paper, the biomimetic mineralization method was used to deposit Sr-doped Sr-HA/PLLA scaffold on the porous scaffold of polylactic acid (PLLA), and the biomimetic mineralized Sr-HA/PLLA scaffold was prepared. Containing a lot of biological inorganic minerals, It provides a more suitable environment for cell adhesion and growth, and better mimics the extracellular matrix composition and structure of natural bone, and provides a better biocompatibility environment for bone repair. The results of contact angle and protein adsorption experiments showed that the deposition of Sr-HA could significantly improve the osseoinductivity of PLLA materials by incorporation of hydrophobicity and Sr, compared with the traditional HA. Both in vivo and in vitro experiments show that the composite has good biocompatibility, promotes osteoblast adhesion, proliferation and differentiation, and induces bone repair.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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