基于微透析的组织液中葡萄糖浓度连续检测方法研究

发布时间：2018-03-20 09:36

本文选题：组织液　切入点：葡萄糖浓度　出处：《天津大学》2012年硕士论文　论文类型：学位论文

【摘要】：血糖检测在糖尿病的诊断治疗中具有重要作用。传统的取血检测疼痛感强,测量结果不连续;无创血糖检测依靠光与人体特定部位组织的相互作用来检测血糖,个体差异性强,信号提取困难;微创血糖检测通过检测组织液中葡萄糖浓度来预测血糖浓度,可实现动态、连续检测,是目前的研究热点。然而,受人体生理活动的影响,组织液中的葡萄糖浓度与血糖浓度存在一定延迟。在研究这一延迟关系时,需要引入二者之间的生理延迟参数,建立延迟模型,而延迟模型需要通过精确测量组织液中的葡萄糖浓度和血糖浓度来进行实验验证。但是,如何连续测量组织液中葡萄糖浓度的精确值目前仍是一个世界难题。本文研究了基于微透析的组织液中葡萄糖浓度连续检测方法,建立了实验系统,开展了体外模拟葡萄糖浓度连续变化的检测实验和动物在体实验,分析了灌流速度、葡萄糖浓度和温度等主要因素对微透析回收率的影响,并通过超滤方法验证了微透析方法用于连续检测组织液中葡萄糖浓度的准确性。具体工作包括: (1)从理论上研究了采用微透析连续检测组织液中葡萄糖浓度的方法,分析了微透析回收率的数学模型,讨论了测量微透析回收率的方法。 (2)建立了基于微透析的葡萄糖浓度检测系统,利用葡萄糖溶液研究了基于微透析的葡萄糖浓度检测方法,分析了灌流速度、葡萄糖浓度以及温度等主要因素对微透析回收率的影响。 (3)建立了模拟人体组织液中葡萄糖浓度连续变化的实验平台,为验证基于微透析的葡萄糖浓度检测技术及仪器奠定了基础。 (4)进行了基于超滤抽取的组织液中葡萄糖浓度检测方法的研究,分析了真空负压、探针膜长和连接管长度等对抽取速率的影响,为开展超滤动物实验奠定了基础。 (5)进行了动物体内的微透析和超滤检测实验,研究了微透析体内回收率的测量方法,并通过超滤方法验证了微透析检测的准确性,为组织液中葡萄糖浓度与血糖浓度延迟模型的研究奠定了基础。
[Abstract]:Blood glucose detection plays an important role in the diagnosis and treatment of diabetes mellitus. The traditional blood sampling test has strong pain, and the measurement results are not continuous. Non-invasive blood glucose detection depends on the interaction between light and specific parts of the human body to detect blood sugar. It is difficult to extract the signal. Microinvasive blood glucose detection can predict glucose concentration by detecting glucose concentration in tissue fluid, which can realize dynamic and continuous detection, which is a hot research topic at present. However, it is affected by physiological activities of human body. There is a certain delay between glucose concentration and blood glucose concentration in tissue solution. In order to study the relationship between glucose concentration and blood glucose concentration, we should introduce the physiological delay parameters between them and establish a delay model. The delay model needs to be verified by accurate measurement of glucose concentration and blood glucose concentration in tissue solution. However, how to continuously measure glucose concentration in tissue fluid is still a difficult problem all over the world. In this paper, the method of continuous measurement of glucose concentration in tissue fluid based on microdialysis was studied, and the experimental system was established. The in vitro and in vivo experiments of simulating the continuous variation of glucose concentration were carried out, and the perfusion rate was analyzed. The effects of glucose concentration and temperature on the recovery rate of microdialysis were studied. The accuracy of microdialysis for continuous determination of glucose concentration in tissue solution was verified by ultrafiltration. 1) the method of continuous determination of glucose concentration in tissue solution by microdialysis was studied theoretically, the mathematical model of recovery rate of microdialysis was analyzed, and the method of measuring the recovery rate of microdialysis was discussed. The glucose concentration detection system based on microdialysis was established. The glucose concentration detection method based on microdialysis was studied by using glucose solution, and the perfusion rate was analyzed. The effect of glucose concentration and temperature on the recovery rate of microdialysis. (3) an experimental platform was established to simulate the continuous variation of glucose concentration in human tissue solution, which laid a foundation for the verification of glucose concentration detection technology and instrument based on microdialysis. The method of measuring glucose concentration in tissue solution based on ultrafiltration was studied. The effects of vacuum negative pressure, the length of probe membrane and the length of connecting pipe on the extraction rate were analyzed, which laid a foundation for the experiment of ultrafiltration animals. In this paper, microdialysis and ultrafiltration tests in animals were carried out, and the method of measuring the recovery rate in vivo was studied, and the accuracy of microdialysis detection was verified by ultrafiltration method. It lays a foundation for the study of the delayed model of glucose concentration and blood glucose concentration in tissue solution.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R446.1;TH776

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