纳米羟基磷灰石对正常细胞和肿瘤细胞的毒性比较研究

发布时间：2018-03-24 10:38

本文选题：纳米羟基磷灰石　切入点：肝细胞　出处：《现代预防医学》2017年11期

【摘要】：目的比较纳米羟基磷灰石(HA-NPs)对肿瘤细胞和正常细胞的毒性差异,为HA-NPs的安全性提供实验证据。方法以肝肿瘤细胞(Hep G2)和肝正常细胞(L02)为研究对象,采用噻唑蓝比色(MTT)、乳酸脱氢酶(LDH)释放和台盼蓝染色三种不同的实验方法比较HA-NPs对Hep G2和L02的细胞毒性差异。结果 MTT结果显示,在HANPs染毒浓度为100、200和400μg/ml时,L02细胞存活率分别为110%、114%和104%,而Hep G2细胞存活率分别为92%、89%和73%;在HA-NPs浓度为600μg/ml~1600μg/ml时,L02细胞的存活率从90%降至27%,而Hep G2细胞的存活率则从67%降至13%,差异均有统计学意义(P0.05)。LDH释放和台盼蓝染色结果显示,400、600和800μg/ml的HA-NPs对Hep G2和L02的细胞毒性均高于相应的对照组(P0.05),且相同剂量的HA-NPs对Hep G2的细胞毒性要高于L02(P0.05)。结论高浓度HA-NPs对Hep G2和L02细胞均具有毒性效应,且相同剂量的HANPs对L02的细胞毒性要低于Hep G2细胞。
[Abstract]:Objective to compare the toxicity of nano-hydroxyapatite (HA-NPs) to tumor cells and normal cells, and to provide experimental evidence for the safety of HA-NPs. Methods Hepatic tumor cells (Hep G2) and normal liver cells (L02) were used as subjects. The cytotoxicity of HA-NPs to Hep G2 and L02 was compared by using three different methods: thiazolyl blue colorimetric assay, lactate dehydrogenase (LDH) release and trypan blue staining. At the concentration of 100200 渭 g/ml~1600 渭 g/ml and 400 渭 g/ml of HANPs, the survival rate of L02 cells was 110% and 104%, respectively, while that of Hep G2 cells was 92 89% and 73%, respectively, while the survival rate of L02 cells decreased from 90% to 27% at HA-NPs concentration of 600 渭 g/ml~1600 渭 g/ml, while the survival rate of Hep G2 cells decreased from 67% to 73%. There were significant differences in the cytotoxicity of Hep G2 and L02 with HA-NPs of 400600 and 800 渭 g/ml, and the cytotoxicity of the same dose of HA-NPs to Hep G2 was higher than that of L02P0.05.The results of trypan blue staining showed that the cytotoxicity of HA-NPs to Hep G2 was higher than that of L02G _ 2. Conclusion the cytotoxicity of HA-NPs to Hep G2 is higher than that of control group (P _ (0.05)). The concentration of HA-NPs had toxic effects on Hep G2 and L02 cells. The cytotoxicity of the same dose of HANPs to L02 was lower than that of Hep G2 cells.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);
【基金】：国家自然科学基金面上项目(NO.81372945)
【分类号】：R318.08

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