生物3D打印多层皮肤组织模型的研究

发布时间：2018-03-24 15:37

  本文选题：生物3D打印　切入点：多层皮肤模型　出处：《杭州电子科技大学》2017年硕士论文

【摘要】：由烧伤、创伤及不愈合溃疡等导致的大面积的皮肤缺损创面如不及时治疗,会造成患者体液大量丢失、继发严重感染,严重威胁患者生命。常用的治疗措施包括自体皮肤移植、异体/异种皮肤移植、细胞活性因子、细胞治疗、真皮替代物等,但传统治疗方法在皮肤修复时间、治疗成本、治疗效果等方面依然存在不足。生物3D打印基于快速成型分层制造的原理,可将细胞及基质材料等物质准确输送与定位,构建具有仿生三维结构及功能的类组织前体,为构建复杂仿生结构的组织模型研究提供了新的技术可能。对比于传统皮肤组织工程技术,生物三维打印技术具有细胞定位精确,逐层打印高效的特点,可使制造周期大幅缩短,提高效率。本研究基于生物3D打印设备,自手术废弃皮片中提取真皮成纤维细胞和表皮角质形成细胞、制备细胞/生物相容性材料复合凝胶,基于皮肤分层结构进行打印,构建与人体皮肤结构类似的组织。研究首先对组织工程皮肤研究现状、生物3D打印技术背景和研究进展做了简要综述,总结现有组织工程皮肤技术局限,对基于生物3D打印技术构建多层皮肤组织可行性做了探讨。其次简单的介绍团队研发的3D Bio-Architect○R-WS组成系统及皮肤打印基质材料的选择,之后对来自人体皮肤的角质形成细胞和成纤维细胞的提取和培养过程进行阐述,然后依据人体皮肤真实的结构,设计人体皮肤三维结构模型,将人体来源的成纤维细胞/胶原/明胶/海藻酸钠作为真皮层、角质形成细胞/Ⅰ型胶原作为表皮层,通过生物3D打印设备严格按照人体皮肤真实结构精准装配组成多层皮肤组织模型。利用MTT比色法检测打印过程对支架中成纤维细胞存活率的影响,其次用荧光染色法和HE染色对人体皮肤分层结构作了研究。研究结果证明,基于生物3D打印技术,能够快速有效的在体外构建高仿真的多层皮肤组织模型。本研究为快速制备具有多层仿生结构的人工皮肤用于皮肤修复、化妆品安全性测试和药物渗透性研究提供了新的方法。
[Abstract]:Large areas of skin defects caused by burns, wounds and nonunion ulcers, if not treated in time, will result in massive loss of body fluids, secondary severe infection and serious threat to the life of the patients. Common treatment measures include autogenous skin transplantation. Allograft / xenogeneic skin transplantation, cytokines, cell therapy, dermal substitutes, etc. Based on the principle of rapid prototyping and layered manufacturing, biological 3D printing can accurately transport and locate materials such as cells and matrix materials, and construct biomimetic tissue precursors with three-dimensional structure and function. Compared with the traditional skin tissue engineering technology, the three dimensional biological printing technology has the characteristics of accurate location of cells and high efficiency of printing layer by layer, compared with the traditional skin tissue engineering technology, which provides a new technique for the study of tissue models of complex bionic structures. In this study, dermal fibroblasts and epidermal keratinocytes were extracted from abandoned skin grafts to prepare cell / biocompatibility composite gel based on biological 3D printing equipment. Based on skin layering structure, tissue similar to human skin structure was constructed. First of all, the present situation of tissue engineering skin, the background and research progress of biological 3D printing technology were briefly reviewed. This paper summarizes the limitations of existing tissue engineering skin technology, and discusses the feasibility of constructing multilayer skin tissue based on biological 3D printing technology. Secondly, the paper briefly introduces the 3D Bio-Architect0R-WS composition system developed by the team and the selection of skin printing matrix materials. After that, the extraction and culture process of keratinocytes and fibroblasts from human skin are described, and then the three-dimensional structure model of human skin is designed according to the real structure of human skin. Human fibroblasts / collagen / gelatin / sodium alginate were used as dermis and keratinocytes / collagen 鈪，

本文编号：1658933